caa a22 4500 605514135 CHVBK 20210128100703.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00360-015-0924-6 doi (NATIONALLICENCE)springer-10.1007/s00360-015-0924-6 Calcium response of KCl-excited populations of ventricular myocytes from the European sea bass ( Dicentrarchus labrax ): a promising approach to integrate cell-to-cell heterogeneity in studying the cellular basis of fish cardiac performance [Elektronische Daten] [Hélène Ollivier, James Marchant, Nicolas Le Bayon, Arianna Servili, Guy Claireaux] Climate change challenges the capacity of fishes to thrive in their habitat. However, through phenotypic diversity, they demonstrate remarkable resilience to deteriorating conditions. In fish populations, inter-individual variation in a number of fitness-determining physiological traits, including cardiac performance, is classically observed. Information about the cellular bases of inter-individual variability in cardiac performance is scarce including the possible contribution of excitation-contraction (EC) coupling. This study aimed at providing insight into EC coupling-related Ca2+ response and thermal plasticity in the European sea bass (Dicentrarchus labrax). A cell population approach was used to lay the methodological basis for identifying the cellular determinants of cardiac performance. Fish were acclimated at 12 and 22°C and changes in intracellular calcium concentration ([Ca2+]i) following KCl stimulation were measured using Fura-2, at 12 or 22°C-test. The increase in [Ca2+]i resulted primarily from extracellular Ca2+ entry but sarcoplasmic reticulum stores were also shown to be involved. As previously reported in sea bass, a modest effect of adrenaline was observed. Moreover, although the response appeared relatively insensitive to an acute temperature change, a difference in Ca2+ response was observed between 12- and 22°C-acclimated fish. In particular, a greater increase in [Ca2+]i at a high level of adrenaline was observed in 22°C-acclimated fish that may be related to an improved efficiency of adrenaline under these conditions. In conclusion, this method allows a rapid screening of cellular characteristics. It represents a promising tool to identify the cellular determinants of inter-individual variability in fishes' capacity for environmental adaptation. Springer-Verlag Berlin Heidelberg, 2015 Sea bass nationallicence Myocyte populations nationallicence Calcium signaling nationallicence Cardiac plasticity nationallicence Inter-individual variability nationallicence Ollivier Hélène ORPHY, Université de Bretagne Occidentale, Brest, France aut Marchant James Unité PFOM-ARN, LEMAR, Centre Ifremer de Brest, Plouzané, France aut Le Bayon Nicolas Unité PFOM-ARN, LEMAR, Centre Ifremer de Brest, Plouzané, France aut Servili Arianna Unité PFOM-ARN, LEMAR, Centre Ifremer de Brest, Plouzané, France aut Claireaux Guy Unité PFOM-ARN, LEMAR, Centre Ifremer de Brest, Plouzané, France aut Journal of Comparative Physiology B Springer Berlin Heidelberg 185/7(2015-10-01), 755-765 0174-1578 185:7<755 2015 185 360 https://doi.org/10.1007/s00360-015-0924-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00360-015-0924-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ollivier Hélène ORPHY, Université de Bretagne Occidentale, Brest, France aut NATIONALLICENCE 700 1- Marchant James Unité PFOM-ARN, LEMAR, Centre Ifremer de Brest, Plouzané, France aut NATIONALLICENCE 700 1- Le Bayon Nicolas Unité PFOM-ARN, LEMAR, Centre Ifremer de Brest, Plouzané, France aut NATIONALLICENCE 700 1- Servili Arianna Unité PFOM-ARN, LEMAR, Centre Ifremer de Brest, Plouzané, France aut NATIONALLICENCE 700 1- Claireaux Guy Unité PFOM-ARN, LEMAR, Centre Ifremer de Brest, Plouzané, France aut NATIONALLICENCE 773 0- Journal of Comparative Physiology B Springer Berlin Heidelberg 185/7(2015-10-01), 755-765 0174-1578 185:7<755 2015 185 360