caa a22 4500 605514186 CHVBK 20210128100703.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00360-014-0884-2 doi (NATIONALLICENCE)springer-10.1007/s00360-014-0884-2 Cold tolerance of the Antarctic nematodes Plectus murrayi and Scottnema lindsayae [Elektronische Daten] [David Wharton, Mélianie Raymond] The cold tolerance of the Antarctic nematodes Scottnema lindsayae and Plectus murrayi was determined using material freshly isolated from the field. Both species could survive low temperatures but the survival of S. lindsayae was greater than that of P. murrayi. Field soil temperatures in late spring—early summer indicated a minimum temperature of −19.5°C and a maximum cooling rate of 0.71°C min−1. In P. murrayi grown in culture, there was no significant effect of acclimation, nor of the two culture media used, on survival after freezing but survival was greater if freezing was seeded at -1°C than at lower temperatures. The freezing survival ability of P. murrayi is much less than that of Panagrolaimus davidi CB1, another Antarctic nematode. Cryomicroscopy indicates that P. murrayi can survive low temperatures by either cryoprotective dehydration or freezing tolerance, but that freezing tolerance is the dominant strategy. Measurable thermal hysteresis was detected only in highly concentrated extracts of the nematodes, indicating the presence of an antifreeze protein, but at the concentrations likely to be found in vivo, the major function of the ice active protein involved is probably recrystallization inhibition. Springer-Verlag Berlin Heidelberg, 2015 Cold tolerance nationallicence Freezing nationallicence Antarctic nationallicence Nematode nationallicence Thermal hysteresis nationallicence Recrystallization inhibition nationallicence ATW : Artificial tap water nationallicence BSS : Balanced salt solution nationallicence CL : Confidence limits nationallicence GLM : General linear model nationallicence $$n_{p}^{2}$$ n p 2 : Partial eta squared nationallicence S50 : 50% survival temperature nationallicence RMP : Relative median potency nationallicence Tmin : Minimum temperature nationallicence Wharton David Department of Zoology, University of Otago, P.O. Box 56, Dunedin, New Zealand aut Raymond Mélianie Department of Zoology, University of Otago, P.O. Box 56, Dunedin, New Zealand aut Journal of Comparative Physiology B Springer Berlin Heidelberg 185/3(2015-04-01), 281-289 0174-1578 185:3<281 2015 185 360 https://doi.org/10.1007/s00360-014-0884-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00360-014-0884-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wharton David Department of Zoology, University of Otago, P.O. Box 56, Dunedin, New Zealand aut NATIONALLICENCE 700 1- Raymond Mélianie Department of Zoology, University of Otago, P.O. Box 56, Dunedin, New Zealand aut NATIONALLICENCE 773 0- Journal of Comparative Physiology B Springer Berlin Heidelberg 185/3(2015-04-01), 281-289 0174-1578 185:3<281 2015 185 360