caa a22 4500 605514232 CHVBK 20210128100703.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00360-014-0883-3 doi (NATIONALLICENCE)springer-10.1007/s00360-014-0883-3 Using biochemical markers to assess the effects of imposed temperature stress on freshwater decapod crustaceans: Cherax quadricarinatus as a test case [Elektronische Daten] [J. Bone, G. Renshaw, J. Furse, C. Wild] The effects of thermal stress can impact negatively on the abundance and distribution of temperature-sensitive species, particularly freshwater crustaceans. This study investigated the effects of thermal stress on physiological and biochemical parameters at five treatment temperatures resulting in minimal (25°C), moderate (27, 29°C) or severe (31, 33°C) thermal stress in the common tropical freshwater crayfish Cherax quadricarinatus. The aim was to develop a suite of stress-sensitive assays to use on threatened populations of freshwater crustaceans, particularly those restricted to cooler temperatures and only found in high altitude refugia. Significant increases in indicators of oxidative and metabolic stress were observed at 29°C and were elevated further at 33°C. After a 50-day acclimation to an imposed temperature stress, significant changes in the level of total glutathione, total lipids, muscular protein, total haemocyte count, lipid peroxidation and protein carbonyls were observed between treatments while superoxide dismutase activity and haemolymph protein concentrations did not change. The data provided proof of concept that measuring key biochemical responses to high temperature can provide a means of contrasting the level of thermal stress experienced between individuals of the same species adapted to different temperatures. The methods developed are expected to be of use in research on wild populations of other freshwater poikilothermic organisms, particularly those susceptible to increased environmental temperatures associated with climate change. Springer-Verlag Berlin Heidelberg, 2014 Poikilothermic nationallicence Oxidative nationallicence Metabolic nationallicence Thermal stress nationallicence Decapod nationallicence Biochemical indicators nationallicence Predicting survival nationallicence Physiological response nationallicence Red claw nationallicence Cherax quadricarinatus nationallicence Bone J. Environmental Futures Research Institute, Griffith School of Environment, Gold Coast Campus, Griffith University, 4222, Gold Coast, QLD, Australia aut Renshaw G. Hypoxia and Ischemia Research Unit, School of Allied Health Sciences, Gold Coast Campus, Griffith University, 4222, Gold Coast, QLD, Australia aut Furse J. Environmental Futures Research Institute, Griffith School of Environment, Gold Coast Campus, Griffith University, 4222, Gold Coast, QLD, Australia aut Wild C. Environmental Futures Research Institute, Griffith School of Environment, Gold Coast Campus, Griffith University, 4222, Gold Coast, QLD, Australia aut Journal of Comparative Physiology B Springer Berlin Heidelberg 185/3(2015-04-01), 291-301 0174-1578 185:3<291 2015 185 360 https://doi.org/10.1007/s00360-014-0883-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00360-014-0883-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bone J. Environmental Futures Research Institute, Griffith School of Environment, Gold Coast Campus, Griffith University, 4222, Gold Coast, QLD, Australia aut NATIONALLICENCE 700 1- Renshaw G. Hypoxia and Ischemia Research Unit, School of Allied Health Sciences, Gold Coast Campus, Griffith University, 4222, Gold Coast, QLD, Australia aut NATIONALLICENCE 700 1- Furse J. Environmental Futures Research Institute, Griffith School of Environment, Gold Coast Campus, Griffith University, 4222, Gold Coast, QLD, Australia aut NATIONALLICENCE 700 1- Wild C. Environmental Futures Research Institute, Griffith School of Environment, Gold Coast Campus, Griffith University, 4222, Gold Coast, QLD, Australia aut NATIONALLICENCE 773 0- Journal of Comparative Physiology B Springer Berlin Heidelberg 185/3(2015-04-01), 291-301 0174-1578 185:3<291 2015 185 360