Maintenance of skeletal muscle energy homeostasis during prolonged wintertime fasting in the raccoon dog ( Nyctereutes procyonoides )
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| 024 | 7 | 0 | |a 10.1007/s00360-015-0893-9 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00360-015-0893-9 | ||
| 245 | 0 | 0 | |a Maintenance of skeletal muscle energy homeostasis during prolonged wintertime fasting in the raccoon dog ( Nyctereutes procyonoides ) |h [Elektronische Daten] |c [Sanni Kinnunen, Satu Mänttäri, Karl-Heinz Herzig, Petteri Nieminen, Anne-Mari Mustonen, Seppo Saarela] |
| 520 | 3 | |a The raccoon dog (Nyctereutes procyonoides) is a canid species with autumnal fattening and prolonged wintertime fasting. Nonpathological body weight cycling and the ability to tolerate food deficiency make this species a unique subject for studying physiological mechanisms in energy metabolism. AMP-activated protein kinase (AMPK) is a cellular energy sensor regulating energy homeostasis. During acute fasting, AMPK promotes fatty acid oxidation and enhances glucose uptake. We evaluated the effects of prolonged fasting on muscle energy metabolism in farm-bred raccoon dogs. Total and phosphorylated AMPK and acetyl-CoA carboxylase (ACC), glucose transporter 4 (GLUT 4), insulin receptor and protein kinase B (Akt) protein expressions of hind limb muscles were determined by Western blot after 10weeks of fasting. Plasma insulin, leptin, ghrelin, glucose and free fatty acid levels were measured, and muscle myosin heavy chain (MHC) isoform composition analyzed. Fasting had no effects on AMPK phosphorylation, but total AMPK expression decreased in m. rectus femoris, m. tibialis anterior and m. extensor digitorum longus resulting in a higher phosphorylation ratio. Decreased total expression was also observed for ACC. Fasting did not influence GLUT 4, insulin receptor or Akt expression, but Akt phosphorylation was lower in m. flexor digitorum superficialis and m. extensor digitorum longus. Three MHC isoforms (I, IIa and IIx) were detected without differences in composition between the fasted and control animals. The studied muscles were resistant to prolonged fasting indicating that raccoon dogs have an effective molecular regulatory system for preserving skeletal muscle function during wintertime immobility and fasting. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a ACC |2 nationallicence | |
| 690 | 7 | |a Akt |2 nationallicence | |
| 690 | 7 | |a AMPK |2 nationallicence | |
| 690 | 7 | |a Energy metabolism |2 nationallicence | |
| 690 | 7 | |a Food deprivation |2 nationallicence | |
| 690 | 7 | |a GLUT 4 |2 nationallicence | |
| 690 | 7 | |a Skeletal muscle |2 nationallicence | |
| 690 | 7 | |a ACC : Acetyl-CoA carboxylase |2 nationallicence | |
| 690 | 7 | |a Akt : Protein kinase B |2 nationallicence | |
| 690 | 7 | |a AMPK : AMP-activated protein kinase |2 nationallicence | |
| 690 | 7 | |a EDL : m. extensor digitorum longus |2 nationallicence | |
| 690 | 7 | |a FDS : m. flexor digitorum superficialis |2 nationallicence | |
| 690 | 7 | |a FFA : Free fatty acid |2 nationallicence | |
| 690 | 7 | |a GA : m. gastrocnemius |2 nationallicence | |
| 690 | 7 | |a GAPDH : Glyceraldehyde 3-phosphate dehydrogenase |2 nationallicence | |
| 690 | 7 | |a GLUT 4 : Glucose transporter 4 |2 nationallicence | |
| 690 | 7 | |a IR : Insulin receptor |2 nationallicence | |
| 690 | 7 | |a MHC : Myosin heavy chain |2 nationallicence | |
| 690 | 7 | |a pAMPK : Phosphorylated AMPK (active form) |2 nationallicence | |
| 690 | 7 | |a RF : m. rectus femoris |2 nationallicence | |
| 690 | 7 | |a SDH : Succinate dehydrogenase |2 nationallicence | |
| 690 | 7 | |a SDS-PAGE : Sodium dodecyl sulfate-polyacrylamide gel electrophoresis |2 nationallicence | |
| 690 | 7 | |a TA : m. tibialis anterior |2 nationallicence | |
| 700 | 1 | |a Kinnunen |D Sanni |u Department of Biology, University of Oulu, P.O. Box 3000, 90014, Oulu, Finland |4 aut | |
| 700 | 1 | |a Mänttäri |D Satu |u Finnish Institute of Occupational Health, Aapistie 1, 90220, Oulu, Finland |4 aut | |
| 700 | 1 | |a Herzig |D Karl-Heinz |u Institute of Biomedicine, Biocenter of Oulu, University of Oulu, P.O. Box 5000, 90014, Oulu, Finland |4 aut | |
| 700 | 1 | |a Nieminen |D Petteri |u Institute of Biomedicine/Anatomy, School of Medicine, Faculty of Health Sciences, University of Eastern Finland, P.O. Box 1627, 70211, Kuopio, Finland |4 aut | |
| 700 | 1 | |a Mustonen |D Anne-Mari |u Institute of Biomedicine/Anatomy, School of Medicine, Faculty of Health Sciences, University of Eastern Finland, P.O. Box 1627, 70211, Kuopio, Finland |4 aut | |
| 700 | 1 | |a Saarela |D Seppo |u Department of Biology, University of Oulu, P.O. Box 3000, 90014, Oulu, Finland |4 aut | |
| 773 | 0 | |t Journal of Comparative Physiology B |d Springer Berlin Heidelberg |g 185/4(2015-05-01), 435-445 |x 0174-1578 |q 185:4<435 |1 2015 |2 185 |o 360 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00360-015-0893-9 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00360-015-0893-9 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kinnunen |D Sanni |u Department of Biology, University of Oulu, P.O. Box 3000, 90014, Oulu, Finland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Mänttäri |D Satu |u Finnish Institute of Occupational Health, Aapistie 1, 90220, Oulu, Finland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Herzig |D Karl-Heinz |u Institute of Biomedicine, Biocenter of Oulu, University of Oulu, P.O. Box 5000, 90014, Oulu, Finland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Nieminen |D Petteri |u Institute of Biomedicine/Anatomy, School of Medicine, Faculty of Health Sciences, University of Eastern Finland, P.O. Box 1627, 70211, Kuopio, Finland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Mustonen |D Anne-Mari |u Institute of Biomedicine/Anatomy, School of Medicine, Faculty of Health Sciences, University of Eastern Finland, P.O. Box 1627, 70211, Kuopio, Finland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Saarela |D Seppo |u Department of Biology, University of Oulu, P.O. Box 3000, 90014, Oulu, Finland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Journal of Comparative Physiology B |d Springer Berlin Heidelberg |g 185/4(2015-05-01), 435-445 |x 0174-1578 |q 185:4<435 |1 2015 |2 185 |o 360 | ||