Temperature-dependence of L-type Ca2+ current in ventricular cardiomyocytes of the Alaska blackfish ( Dallia pectoralis )
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| 024 | 7 | 0 | |a 10.1007/s00360-015-0931-7 |2 doi |
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| 245 | 0 | 0 | |a Temperature-dependence of L-type Ca2+ current in ventricular cardiomyocytes of the Alaska blackfish ( Dallia pectoralis ) |h [Elektronische Daten] |c [Kerry Kubly, Jonathan Stecyk] |
| 520 | 3 | |a To lend insight into the overwintering strategy of the Alaska blackfish (Dallia pectoralis), we acclimated fish to 15 or 5°C and then utilized whole-cell patch clamp to characterize the effects of thermal acclimation and acute temperature change on the density and kinetics of ventricular L-type Ca2+ current (I Ca). Peak I Ca density at 5°C (−1.1±0.1pApF−1) was 1/8th that at 15°C (−8.8±0.6pApF−1). However, alterations of the Ca2+- and voltage-dependent inactivation properties of L-type Ca2+ channels partially compensated against the decrease. The time constant tau (τ) for the kinetics of inactivation of I Ca was ~4.5 times greater at 5°C than at 15°C, and the voltage for half-maximal inactivation was shifted from −23.3±1.0mV at 15°C to −19.8±1.2mV at 5°C. These modifications increase the open probability of the channel and culminate in an approximate doubling of the L-type Ca2+ window current, which contributes to approximately 15% of the maximal Ca2+ conductance at 5°C. Consequently, the charge density of I Ca (Q Ca) and the total Ca2+ transferred through the L-type Ca2+ channels (Δ[Ca2+]) were not as severely reduced at 5°C as compared to peak I Ca density. In combination, the results suggest that while the Alaska blackfish substantially down-regulates I Ca with acclimation to low temperature, there is sufficient compensation in the kinetics of the L-type Ca2+ channel to support the level of cardiac performance required for the fish to remain active throughout the winter. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Electrophysiology |2 nationallicence | |
| 690 | 7 | |a I Ca |2 nationallicence | |
| 690 | 7 | |a Heart |2 nationallicence | |
| 690 | 7 | |a Thermal acclimation |2 nationallicence | |
| 700 | 1 | |a Kubly |D Kerry |u Department of Biological Sciences, University of Alaska Anchorage, 3211 Providence Drive, Conoco Philips Integrated Science Building, 99508, Anchorage, AK, USA |4 aut | |
| 700 | 1 | |a Stecyk |D Jonathan |u Department of Biological Sciences, University of Alaska Anchorage, 3211 Providence Drive, Conoco Philips Integrated Science Building, 99508, Anchorage, AK, USA |4 aut | |
| 773 | 0 | |t Journal of Comparative Physiology B |d Springer Berlin Heidelberg |g 185/8(2015-12-01), 845-858 |x 0174-1578 |q 185:8<845 |1 2015 |2 185 |o 360 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00360-015-0931-7 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00360-015-0931-7 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kubly |D Kerry |u Department of Biological Sciences, University of Alaska Anchorage, 3211 Providence Drive, Conoco Philips Integrated Science Building, 99508, Anchorage, AK, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Stecyk |D Jonathan |u Department of Biological Sciences, University of Alaska Anchorage, 3211 Providence Drive, Conoco Philips Integrated Science Building, 99508, Anchorage, AK, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Journal of Comparative Physiology B |d Springer Berlin Heidelberg |g 185/8(2015-12-01), 845-858 |x 0174-1578 |q 185:8<845 |1 2015 |2 185 |o 360 | ||