caa a22 4500 60551447X CHVBK 20210128100704.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00360-015-0906-8 doi (NATIONALLICENCE)springer-10.1007/s00360-015-0906-8 The metabolic rate of cultured muscle cells from hybrid Coturnix quail is intermediate to that of muscle cells from fast-growing and slow-growing Coturnix quail [Elektronische Daten] [Clara Cooper-Mullin, Ana Jimenez, Nicholas Anthony, Matthew Wortman, Joseph Williams] Growth rate is a fundamental parameter of an organism's life history and varies 30-fold across bird species. To explore how whole-organism growth rate and the metabolic rate of cultured muscle cells are connected, two lines of Japanese quail (Coturnix coturnix japonica), one that had been artificially selected for fast growth for over 60 generations and a control line were used to culture myoblasts. In line with previous work, myoblasts from the fast growth line had significantly higher rates of oxygen consumption, glycolytic flux, and higher mitochondrial volume than myoblasts from the control line, indicating that an increase in growth rate is associated with a concomitant increase in cellular metabolic rates and that mitochondrial density contributes to the differences in rates of metabolism between the lines. We reared chicks from two hybrid lines with reciprocal parental configurations for growth rate to explore the effect of maternally inherited mitochondrial DNA on rates of growth and metabolism. Growth rates of chicks, cellular basal oxygen consumption, glycolytic flux, and mitochondrial volume in myoblasts from chicks from both reciprocal crosses were intermediate to the fast and control lines. This indicates that genes in the nucleus have a strong influence on metabolic rates at the cellular level, compared with maternally inherited mitochondrial DNA. Springer-Verlag Berlin Heidelberg, 2015 Cellular metabolic rate nationallicence Myoblast nationallicence Growth rate nationallicence Japanese quail nationallicence Mitochondria nationallicence mtDNA : Mitochondrial DNA nationallicence C : Control line of quail nationallicence F : Fast line of quail nationallicence CFFM : Females from a control line of quail crossed with males from the fast line nationallicence FFCM : Females from the fast line of quail crossed with males from the control line nationallicence CS : Chicken Serum nationallicence HS : Horse Serum nationallicence AbAm : Antibiotic/Antimycotic nationallicence OCR : Oxygen consumption rates nationallicence FCCP : Carbonylcyanide-p-trifluoromethoxyphenylhydrazone nationallicence RCR : Respiratory control ratio nationallicence ECAR : Extracellular acidification rates nationallicence K : Growth rate constant (K) nationallicence DAPI : 4′,6-Diamidino-2-phenylindole nationallicence BMR : Basal metabolic rate nationallicence Cooper-Mullin Clara Evolution, Ecology and Organismal Biology, The Ohio State University, Columbus, OH, USA aut Jimenez Ana Evolution, Ecology and Organismal Biology, The Ohio State University, Columbus, OH, USA aut Anthony Nicholas Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR, USA aut Wortman Matthew Internal Medicine, University of Cincinnati, Cincinnati, OH, USA aut Williams Joseph Evolution, Ecology and Organismal Biology, The Ohio State University, Columbus, OH, USA aut Journal of Comparative Physiology B Springer Berlin Heidelberg 185/5(2015-07-01), 547-557 0174-1578 185:5<547 2015 185 360 https://doi.org/10.1007/s00360-015-0906-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00360-015-0906-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cooper-Mullin Clara Evolution, Ecology and Organismal Biology, The Ohio State University, Columbus, OH, USA aut NATIONALLICENCE 700 1- Jimenez Ana Evolution, Ecology and Organismal Biology, The Ohio State University, Columbus, OH, USA aut NATIONALLICENCE 700 1- Anthony Nicholas Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR, USA aut NATIONALLICENCE 700 1- Wortman Matthew Internal Medicine, University of Cincinnati, Cincinnati, OH, USA aut NATIONALLICENCE 700 1- Williams Joseph Evolution, Ecology and Organismal Biology, The Ohio State University, Columbus, OH, USA aut NATIONALLICENCE 773 0- Journal of Comparative Physiology B Springer Berlin Heidelberg 185/5(2015-07-01), 547-557 0174-1578 185:5<547 2015 185 360