caa a22 4500 605514496 CHVBK 20210128100705.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00360-015-0899-3 doi (NATIONALLICENCE)springer-10.1007/s00360-015-0899-3 Biochemical characteristics and modulation by external and internal factors of aminopeptidase-N activity in the hepatopancreas of a euryhaline burrowing crab [Elektronische Daten] [M. Michiels, J. del Valle, A. López Mañanes] Strikingly, in spite of its physiological importance, information about occurrence, biochemical characteristics and mechanisms of regulation of aminopeptidase-N (APN) in the hepatopancreas of intertidal euryhaline crabs is still lacking. In this work, we determined the occurrence, biochemical characteristics, response to environmental salinity and dopamine of APN in the hepatopancreas of the euryhaline crab Neohelice granulata (Dana 1851) from the open mudflat of Mar Chiquita coastal lagoon (Buenos Aires province, Argentina). APN activity was maximal at pH and temperature range of 7.6-9.0 and 37-45°C, respectively. APN activity exhibited Michaelis-Menten kinetics (apparent Km=0.19±0.10mM) (pH 7.6, 37°C) and appeared to be sensitive to bestatin (I 50=15mM) and EDTA (I 50=9mM). In crabs acclimated to 10psu (hyper-regulation conditions) and 37psu (hypo-regulation conditions), APN activity was about 45 and 160% higher, respectively, than in 35psu (osmoconformation). APN activity in the hepatopancreas was stimulated in vitro (about 137%) by 10−4 M dopamine. Higher dopamine concentrations produced a similar extent of increase. The responses of APN activity to salinity and dopamine in vitro suggest the role of APN in digestive adjustments upon hyper and hypo-regulatory conditions and its modulation via direct mechanisms on hepatopancreas by dopamine. Springer-Verlag Berlin Heidelberg, 2015 Aminopeptidase N nationallicence Hepatopancreas nationallicence Environment nationallicence Dopamine nationallicence Michiels M. Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Instituto de Investigaciones Marinas y Costeras (IIMyC), Consejo Nacional de Investigaciones Científicas y Tecnológias (CONICET), Universidad Nacional de Mar del Plata, Mar del Plata, Argentina aut del Valle J. Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Instituto de Investigaciones Marinas y Costeras (IIMyC), Consejo Nacional de Investigaciones Científicas y Tecnológias (CONICET), Universidad Nacional de Mar del Plata, Mar del Plata, Argentina aut López Mañanes A. Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Instituto de Investigaciones Marinas y Costeras (IIMyC), Consejo Nacional de Investigaciones Científicas y Tecnológias (CONICET), Universidad Nacional de Mar del Plata, Mar del Plata, Argentina aut Journal of Comparative Physiology B Springer Berlin Heidelberg 185/5(2015-07-01), 501-510 0174-1578 185:5<501 2015 185 360 https://doi.org/10.1007/s00360-015-0899-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00360-015-0899-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Michiels M. Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Instituto de Investigaciones Marinas y Costeras (IIMyC), Consejo Nacional de Investigaciones Científicas y Tecnológias (CONICET), Universidad Nacional de Mar del Plata, Mar del Plata, Argentina aut NATIONALLICENCE 700 1- del Valle J. Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Instituto de Investigaciones Marinas y Costeras (IIMyC), Consejo Nacional de Investigaciones Científicas y Tecnológias (CONICET), Universidad Nacional de Mar del Plata, Mar del Plata, Argentina aut NATIONALLICENCE 700 1- López Mañanes A. Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Instituto de Investigaciones Marinas y Costeras (IIMyC), Consejo Nacional de Investigaciones Científicas y Tecnológias (CONICET), Universidad Nacional de Mar del Plata, Mar del Plata, Argentina aut NATIONALLICENCE 773 0- Journal of Comparative Physiology B Springer Berlin Heidelberg 185/5(2015-07-01), 501-510 0174-1578 185:5<501 2015 185 360