Functional characterization of a putative disaccharide membrane transporter in crustacean intestine
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| 024 | 7 | 0 | |a 10.1007/s00360-014-0876-2 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00360-014-0876-2 | ||
| 245 | 0 | 0 | |a Functional characterization of a putative disaccharide membrane transporter in crustacean intestine |h [Elektronische Daten] |c [Rasheda Likely, Eric Johnson, Gregory Ahearn] |
| 520 | 3 | |a Transepithelial absorption of dietary sucrose in the American lobster, Homarus americanus, was investigated by mounting an intestine in a perfusion chamber to characterize mucosal to serosal (MS) 14C-sucrose transport. These fluxes were measured by adding varying concentrations of 14C-sucrose to the perfusate and monitoring their appearance in the bathing solution. Transepithelial 14C-sucrose transport was the combination of a hyperbolic function of luminal concentration, following Michaelis-Menten kinetics, and apparent diffusion. The kinetic constants of the putative sucrose transporter were K M =20.50±6.00µM and J max=1.81±0.50pmol/cm2×min. Phloridzin, an inhibitor of Na+-dependent mucosal glucose transport, decreased MS 14C-sucrose transport. Decreased MS 14C-sucrose transport also occurred in the presence of luminal trehalose, a disaccharide containing d-glucose moieties. Thin-layer chromatography (TLC) identified the chemical nature of radioactively labeled sugars in the bath following transepithelial transport. TLC revealed 14C-sucrose was transported across the intestine largely intact with no 14C-glucose or 14C-fructose appearing in the serosal bath or luminal perfusate. Only 13% of bath radioactivity was volatile metabolites. Results suggest that disaccharide sugars can be transported intact across crustacean intestine and support the occurrence of a functional disaccharide membrane transporter. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Disaccharide transport |2 nationallicence | |
| 690 | 7 | |a Sucrose transport |2 nationallicence | |
| 690 | 7 | |a Intestinal sugar transport |2 nationallicence | |
| 690 | 7 | |a Crustacean |2 nationallicence | |
| 690 | 7 | |a Lobster |2 nationallicence | |
| 690 | 7 | |a Homarus americanus |2 nationallicence | |
| 690 | 7 | |a Phloridzin |2 nationallicence | |
| 690 | 7 | |a Trehalose |2 nationallicence | |
| 690 | 7 | |a SCRT |2 nationallicence | |
| 700 | 1 | |a Likely |D Rasheda |u Department of Biology, University of North Florida, 1 UNF Drive, 32224, Jacksonville, FL, USA |4 aut | |
| 700 | 1 | |a Johnson |D Eric |u Department of Biology, University of North Florida, 1 UNF Drive, 32224, Jacksonville, FL, USA |4 aut | |
| 700 | 1 | |a Ahearn |D Gregory |u Department of Biology, University of North Florida, 1 UNF Drive, 32224, Jacksonville, FL, USA |4 aut | |
| 773 | 0 | |t Journal of Comparative Physiology B |d Springer Berlin Heidelberg |g 185/2(2015-02-01), 173-183 |x 0174-1578 |q 185:2<173 |1 2015 |2 185 |o 360 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00360-014-0876-2 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00360-014-0876-2 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Likely |D Rasheda |u Department of Biology, University of North Florida, 1 UNF Drive, 32224, Jacksonville, FL, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Johnson |D Eric |u Department of Biology, University of North Florida, 1 UNF Drive, 32224, Jacksonville, FL, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ahearn |D Gregory |u Department of Biology, University of North Florida, 1 UNF Drive, 32224, Jacksonville, FL, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Journal of Comparative Physiology B |d Springer Berlin Heidelberg |g 185/2(2015-02-01), 173-183 |x 0174-1578 |q 185:2<173 |1 2015 |2 185 |o 360 | ||