caa a22 4500 605514569 CHVBK 20210128100705.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00360-014-0876-2 doi (NATIONALLICENCE)springer-10.1007/s00360-014-0876-2 Functional characterization of a putative disaccharide membrane transporter in crustacean intestine [Elektronische Daten] [Rasheda Likely, Eric Johnson, Gregory Ahearn] Transepithelial absorption of dietary sucrose in the American lobster, Homarus americanus, was investigated by mounting an intestine in a perfusion chamber to characterize mucosal to serosal (MS) 14C-sucrose transport. These fluxes were measured by adding varying concentrations of 14C-sucrose to the perfusate and monitoring their appearance in the bathing solution. Transepithelial 14C-sucrose transport was the combination of a hyperbolic function of luminal concentration, following Michaelis-Menten kinetics, and apparent diffusion. The kinetic constants of the putative sucrose transporter were K M =20.50±6.00µM and J max=1.81±0.50pmol/cm2×min. Phloridzin, an inhibitor of Na+-dependent mucosal glucose transport, decreased MS 14C-sucrose transport. Decreased MS 14C-sucrose transport also occurred in the presence of luminal trehalose, a disaccharide containing d-glucose moieties. Thin-layer chromatography (TLC) identified the chemical nature of radioactively labeled sugars in the bath following transepithelial transport. TLC revealed 14C-sucrose was transported across the intestine largely intact with no 14C-glucose or 14C-fructose appearing in the serosal bath or luminal perfusate. Only 13% of bath radioactivity was volatile metabolites. Results suggest that disaccharide sugars can be transported intact across crustacean intestine and support the occurrence of a functional disaccharide membrane transporter. Springer-Verlag Berlin Heidelberg, 2014 Disaccharide transport nationallicence Sucrose transport nationallicence Intestinal sugar transport nationallicence Crustacean nationallicence Lobster nationallicence Homarus americanus nationallicence Phloridzin nationallicence Trehalose nationallicence SCRT nationallicence Likely Rasheda Department of Biology, University of North Florida, 1 UNF Drive, 32224, Jacksonville, FL, USA aut Johnson Eric Department of Biology, University of North Florida, 1 UNF Drive, 32224, Jacksonville, FL, USA aut Ahearn Gregory Department of Biology, University of North Florida, 1 UNF Drive, 32224, Jacksonville, FL, USA aut Journal of Comparative Physiology B Springer Berlin Heidelberg 185/2(2015-02-01), 173-183 0174-1578 185:2<173 2015 185 360 https://doi.org/10.1007/s00360-014-0876-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00360-014-0876-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Likely Rasheda Department of Biology, University of North Florida, 1 UNF Drive, 32224, Jacksonville, FL, USA aut NATIONALLICENCE 700 1- Johnson Eric Department of Biology, University of North Florida, 1 UNF Drive, 32224, Jacksonville, FL, USA aut NATIONALLICENCE 700 1- Ahearn Gregory Department of Biology, University of North Florida, 1 UNF Drive, 32224, Jacksonville, FL, USA aut NATIONALLICENCE 773 0- Journal of Comparative Physiology B Springer Berlin Heidelberg 185/2(2015-02-01), 173-183 0174-1578 185:2<173 2015 185 360