The effect of temperature on the rate, affinity, and 15N fractionation of NO3 − during biological denitrification in soils
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| 024 | 7 | 0 | |a 10.1007/s10533-015-0095-2 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10533-015-0095-2 | ||
| 245 | 0 | 4 | |a The effect of temperature on the rate, affinity, and 15N fractionation of NO3 − during biological denitrification in soils |h [Elektronische Daten] |c [Federico Maggi, William Riley] |
| 520 | 3 | |a Nine independent experiments of NO3 − denitrification were analysed using the Arrhenius law and the Eyring's transition-state theory to highlight how temperature affects reaction rate constants, affinities, and kinetic isotopic effects. For temperatures between 20 and 35°C, the Arrhenius law and the transition-state theory described equally well observed temperature increases in 14NO3 − and 15NO3 −denitrification rates (R>0.99 and residuals NRMSE<3.39%, p<0.01). These increases were partly caused by an increase in frequency factor and a slight decrease in activation energy (enthalpy and entropy). Parametric analysis also showed that the affinity of 14NO3 − and 15NO3 − toward a microbial enzyme increased exponentially with temperature and a strong correlation with the rate constants was found (R=0.93, p<0.01). Experimental time- and temperature-averaged fractionation factor α P/S showed only a slight increase with increasing temperature (i.e. lower isotopic effects); however, a comprehensive sensitivity analysis in the concentration-temperature domain using average thermodynamic quantities estimated here showed a more complex response; α P/S was relatively constant for initial bulk concentrations [NO3 −]0≤0.01molkg−1, while substantial nonlinearities developed for [NO3 −]0≥0.01molkg−1 and appeared to be strongly correlated with microbial biomass, whose concentration and activity varied primarily as a function of temperature and available substrate. Values of α P/S ranging between 0.9 and 0.98 for the tested temperatures suggested that interpretations of environmental isotopic signatures should include a sensitivity analysis to the temperature as this affects directly the rate constants and affinities in biochemical reactions and may hide process- and source-related isotopic effects. | |
| 540 | |a Springer International Publishing Switzerland, 2015 | ||
| 690 | 7 | |a Denitrification |2 nationallicence | |
| 690 | 7 | |a 14N and 15N |2 nationallicence | |
| 690 | 7 | |a Kinetic isotopic effects |2 nationallicence | |
| 690 | 7 | |a Affinity |2 nationallicence | |
| 690 | 7 | |a Temperature |2 nationallicence | |
| 690 | 7 | |a Arrhenius |2 nationallicence | |
| 690 | 7 | |a Transition-state theory |2 nationallicence | |
| 690 | 7 | |a α : Fractionation factor (-) |2 nationallicence | |
| 690 | 7 | |a δ : Isotopic composition (-) |2 nationallicence | |
| 690 | 7 | |a x, y : Stoichiometric coefficients (-) |2 nationallicence | |
| 690 | 7 | |a G : Gibbs free energy (Jmol−1) |2 nationallicence | |
| 690 | 7 | |a h : Planck constant (Js) |2 nationallicence | |
| 690 | 7 | |a H : Heat content (Jmol−1) |2 nationallicence | |
| 690 | 7 | |a k : Reaction rate constant (Τ−1) |2 nationallicence | |
| 690 | 7 | |a K b : Boltzmann constant (JK−1 mol−1) |2 nationallicence | |
| 690 | 7 | |a K : Affinity (half-saturation concentration) (M) |2 nationallicence | |
| 690 | 7 | |a S : Entropy content (JK−1 mol−1) |2 nationallicence | |
| 690 | 7 | |a t : Time (T) |2 nationallicence | |
| 690 | 7 | |a T : Absolute temperature (K) |2 nationallicence | |
| 690 | 7 | |a NRMSE : Normalized root mean square error (-) |2 nationallicence | |
| 690 | 7 | |a R : Correlation coefficient (-) |2 nationallicence | |
| 700 | 1 | |a Maggi |D Federico |u Laboratory for Advanced Environmental Engineering Research, School of Civil Engineering, The University of Sydney, 2006, Sydney, NSW, Australia |4 aut | |
| 700 | 1 | |a Riley |D William |u Earth Systems Division, Climate and Carbon Department, Lawrence Berkeley National Laboratory, 94720, Berkeley, CA, USA |4 aut | |
| 773 | 0 | |t Biogeochemistry |d Springer International Publishing |g 124/1-3(2015-05-01), 235-253 |x 0168-2563 |q 124:1-3<235 |1 2015 |2 124 |o 10533 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10533-015-0095-2 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10533-015-0095-2 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Maggi |D Federico |u Laboratory for Advanced Environmental Engineering Research, School of Civil Engineering, The University of Sydney, 2006, Sydney, NSW, Australia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Riley |D William |u Earth Systems Division, Climate and Carbon Department, Lawrence Berkeley National Laboratory, 94720, Berkeley, CA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biogeochemistry |d Springer International Publishing |g 124/1-3(2015-05-01), 235-253 |x 0168-2563 |q 124:1-3<235 |1 2015 |2 124 |o 10533 | ||