caa a22 4500 605516812 CHVBK 20210128100716.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s10533-014-0026-7 doi (NATIONALLICENCE)springer-10.1007/s10533-014-0026-7 Methane fluxes and the functional groups of methanotrophs and methanogens in a young Arctic landscape on Disko Island, West Greenland [Elektronische Daten] [Jesper Christiansen, Alejandro Romero, Niels Jørgensen, Mikkel Glaring, Christian Jørgensen, Louise Berg, Bo Elberling] Arctic soils are known to be important methane (CH4) consumers and sources. This study integrates in situ fluxes of CH4 between upland and wetland soils with potential rates of CH4 oxidation and production as well as abundance and diversity of the methanotrophs and methanogens measured with pyrosequencing of 16S DNA and rRNA fragments in soil and permafrost layers. Here, the spatial patterns of in situ CH4 fluxes for a 2,000years old Arctic landscape in West Greenland reveal similar CH4 uptake rates (−4±0.3μmolm−2h−1) as in other Arctic sites, but lower CH4 emissions (14±1.5μmolm−2h−1) at wetland sites compared to other Arctic wetlands. Potential CH4 oxidation was similar for upland and wetland soils, but the wetter soils produced more CH4 in active and permafrost layers. Accordingly, the abundance of methanogenic archaea was highest in wetland soils. The methanotrophic community also differed between upland and wetland soils, with predominant activity of Type II methanotrophs in the active layer for upland soils, but only Type I methanotrophs for the wetland. In the permafrost of upland and wetland soils, activity of the methanotrophs belonging to Type I and Type II as well as methanogens were detected. This study indicates that the magnitude of CH4 oxidation and the direction of the flux, i.e. uptake or emission, are linked to different methanotrophic communities in upland and wetland soils. Also, the observed link between production/consumption rates and the microbial abundance and activity indicates that the age of an Arctic landscape is not important for the CH4 consumption but can be very important for CH4 production. Considering the prevalence of dry landscapes and contrasting ages of high Arctic soils, our results highlight that well-drained soils should not be overlooked as an important component of Arctic net CH4 budget. Springer International Publishing Switzerland, 2014 Arctic nationallicence Methane fluxes nationallicence Methanotrophs nationallicence Methanogens nationallicence Pyrosequencing nationallicence Soil nationallicence Christiansen Jesper Center for Permafrost (CENPERM), Department of Geosciences and Natural Resource Management, University of Copenhagen, Øster Voldgade 10, 1350, Copenhagen, Denmark aut Romero Alejandro Center for Permafrost (CENPERM), Department of Geosciences and Natural Resource Management, University of Copenhagen, Øster Voldgade 10, 1350, Copenhagen, Denmark aut Jørgensen Niels Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg, Denmark aut Glaring Mikkel Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg, Denmark aut Jørgensen Christian Center for Permafrost (CENPERM), Department of Geosciences and Natural Resource Management, University of Copenhagen, Øster Voldgade 10, 1350, Copenhagen, Denmark aut Berg Louise Center for Permafrost (CENPERM), Department of Geosciences and Natural Resource Management, University of Copenhagen, Øster Voldgade 10, 1350, Copenhagen, Denmark aut Elberling Bo Center for Permafrost (CENPERM), Department of Geosciences and Natural Resource Management, University of Copenhagen, Øster Voldgade 10, 1350, Copenhagen, Denmark aut Biogeochemistry Springer International Publishing 122/1(2015-01-01), 15-33 0168-2563 122:1<15 2015 122 10533 https://doi.org/10.1007/s10533-014-0026-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10533-014-0026-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Christiansen Jesper Center for Permafrost (CENPERM), Department of Geosciences and Natural Resource Management, University of Copenhagen, Øster Voldgade 10, 1350, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Romero Alejandro Center for Permafrost (CENPERM), Department of Geosciences and Natural Resource Management, University of Copenhagen, Øster Voldgade 10, 1350, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Jørgensen Niels Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg, Denmark aut NATIONALLICENCE 700 1- Glaring Mikkel Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg, Denmark aut NATIONALLICENCE 700 1- Jørgensen Christian Center for Permafrost (CENPERM), Department of Geosciences and Natural Resource Management, University of Copenhagen, Øster Voldgade 10, 1350, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Berg Louise Center for Permafrost (CENPERM), Department of Geosciences and Natural Resource Management, University of Copenhagen, Øster Voldgade 10, 1350, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Elberling Bo Center for Permafrost (CENPERM), Department of Geosciences and Natural Resource Management, University of Copenhagen, Øster Voldgade 10, 1350, Copenhagen, Denmark aut NATIONALLICENCE 773 0- Biogeochemistry Springer International Publishing 122/1(2015-01-01), 15-33 0168-2563 122:1<15 2015 122 10533