caa a22 4500 605517231 CHVBK 20210128100718.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s10533-014-0056-1 doi (NATIONALLICENCE)springer-10.1007/s10533-014-0056-1 Concentrations, turnover rates and fluxes of polyamines in coastal waters of the South Atlantic Bight [Elektronische Daten] [Qian Liu, Xinxin Lu, Bradley Tolar, Xiaozhen Mou, James Hollibaugh] Polyamines are short-chain aliphatic compounds containing multiple amine groups. They are important components of the cytosol of eukaryotes and are present at mmolL−1 concentrations inside phytoplankton cells, while complex polyamines play a role in biosilica deposition. Concentrations of polyamines measured in seawater are typically in the sub-nmolL−1 range, implying rapid and efficient uptake by osmotrophs, likely bacterioplankton. We measured turnover rates of three polyamines (putrescine, spermidine and spermine) using 3H-labeled compounds and determined their concentrations by HPLC to estimate polyamine contributions to dissolved organic matter and bacterioplankton carbon and nitrogen demand. These measurements were made on transects from the inner shelf to the Gulf Stream across the South Atlantic Bight (SAB) during April and October of 2011 and in salt marsh estuaries on the Georgia coast during August of 2011 and April of 2012. We found that turnover rates of polyamines were similar to those of amino acids (arginine and glutamic acid) measured in the same samples; however, fluxes of polyamines into bacterioplankton were much lower than amino acid fluxes as a result of low ambient concentrations. Turnover rates and fluxes of polyamines decreased from near-shore waters to the shelf-break, following the pattern of chlorophyll a concentration. Polyamine uptake accounted for less than 10% of bacterial N demand and 5% of bacterial C demand on average, with a large variation among water masses. Springer International Publishing Switzerland, 2014 Polyamines nationallicence Dissolved organic nitrogen nationallicence Bacterioplankton nationallicence Spermidine nationallicence Spermine nationallicence Putrescine nationallicence DFAA nationallicence South Atlantic Bight nationallicence Liu Qian Department of Marine Sciences, University of Georgia, 30602-3636, Athens, GA, USA aut Lu Xinxin Department of Biological Sciences, Kent State University, 44242, Kent, OH, USA aut Tolar Bradley Department of Marine Sciences, University of Georgia, 30602-3636, Athens, GA, USA aut Mou Xiaozhen Department of Biological Sciences, Kent State University, 44242, Kent, OH, USA aut Hollibaugh James Department of Marine Sciences, University of Georgia, 30602-3636, Athens, GA, USA aut Biogeochemistry Springer International Publishing 123/1-2(2015-03-01), 117-133 0168-2563 123:1-2<117 2015 123 10533 https://doi.org/10.1007/s10533-014-0056-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10533-014-0056-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Qian Department of Marine Sciences, University of Georgia, 30602-3636, Athens, GA, USA aut NATIONALLICENCE 700 1- Lu Xinxin Department of Biological Sciences, Kent State University, 44242, Kent, OH, USA aut NATIONALLICENCE 700 1- Tolar Bradley Department of Marine Sciences, University of Georgia, 30602-3636, Athens, GA, USA aut NATIONALLICENCE 700 1- Mou Xiaozhen Department of Biological Sciences, Kent State University, 44242, Kent, OH, USA aut NATIONALLICENCE 700 1- Hollibaugh James Department of Marine Sciences, University of Georgia, 30602-3636, Athens, GA, USA aut NATIONALLICENCE 773 0- Biogeochemistry Springer International Publishing 123/1-2(2015-03-01), 117-133 0168-2563 123:1-2<117 2015 123 10533