Rates and pathways of methanogenesis in hypersaline environments as determined by 13C-labeling

Verfasser / Beitragende:
[Cheryl Kelley, Jeffrey Chanton, Brad Bebout]
Ort, Verlag, Jahr:
2015
Enthalten in:
Biogeochemistry, 126/3(2015-12-01), 329-341
Format:
Artikel (online)
ID: 605517355
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024 7 0 |a 10.1007/s10533-015-0161-9  |2 doi 
035 |a (NATIONALLICENCE)springer-10.1007/s10533-015-0161-9 
245 0 0 |a Rates and pathways of methanogenesis in hypersaline environments as determined by 13C-labeling  |h [Elektronische Daten]  |c [Cheryl Kelley, Jeffrey Chanton, Brad Bebout] 
520 3 |a Rates and pathways of methane production were determined from photosynthetic soft microbial mats and gypsum-encrusted endoevaporites collected in hypersaline environments from California, Mexico and Chile, as well as an organic-rich mud from a pond in the El Tatio volcanic fields, Chile. Samples (mud, soft mats and endoevaporites) were incubated anaerobically with deoxygenated site water, and the increase in methane concentration through time in the headspaces of the incubation vials was used to determine methane production rates. To ascertain the substrates used by the methanogens, 13C-labeled methylamines, methanol, dimethylsulfide, acetate or bicarbonate were added to the incubations (one substrate per vial) and the stable isotopic composition of the resulting methane was measured. The vials amended with 13C-labeled methylamines produced the most 13C-enriched methane, generally followed by the 13C-labeled methanol-amended vials. The stable isotope data and the methane production rates were used to determine first order rate constants for each of the substrates at each of the sites. Estimates of individual substrate use revealed that the methylamines produced 55-92% of the methane generated, while methanol was responsible for another 8-40%. 
540 |a Springer International Publishing Switzerland, 2015 
690 7 |a 13C-labeling  |2 nationallicence 
690 7 |a Hypersaline environments  |2 nationallicence 
690 7 |a Methane  |2 nationallicence 
690 7 |a Stable carbon isotopes  |2 nationallicence 
700 1 |a Kelley  |D Cheryl  |u Department of Geological Sciences, University of Missouri, 65211, Columbia, MO, USA  |4 aut 
700 1 |a Chanton  |D Jeffrey  |u Department of Earth, Ocean and Atmospheric Science, Florida State University, 32306, Tallahassee, FL, USA  |4 aut 
700 1 |a Bebout  |D Brad  |u Exobiology Branch, NASA Ames Research Center, 94035, Moffett Field, CA, USA  |4 aut 
773 0 |t Biogeochemistry  |d Springer International Publishing  |g 126/3(2015-12-01), 329-341  |x 0168-2563  |q 126:3<329  |1 2015  |2 126  |o 10533 
856 4 0 |u https://doi.org/10.1007/s10533-015-0161-9  |q text/html  |z Onlinezugriff via DOI 
898 |a BK010053  |b XK010053  |c XK010000 
900 7 |a Metadata rights reserved  |b Springer special CC-BY-NC licence  |2 nationallicence 
908 |D 1  |a research-article  |2 jats 
949 |B NATIONALLICENCE  |F NATIONALLICENCE  |b NL-springer 
950 |B NATIONALLICENCE  |P 856  |E 40  |u https://doi.org/10.1007/s10533-015-0161-9  |q text/html  |z Onlinezugriff via DOI 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Kelley  |D Cheryl  |u Department of Geological Sciences, University of Missouri, 65211, Columbia, MO, USA  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Chanton  |D Jeffrey  |u Department of Earth, Ocean and Atmospheric Science, Florida State University, 32306, Tallahassee, FL, USA  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Bebout  |D Brad  |u Exobiology Branch, NASA Ames Research Center, 94035, Moffett Field, CA, USA  |4 aut 
950 |B NATIONALLICENCE  |P 773  |E 0-  |t Biogeochemistry  |d Springer International Publishing  |g 126/3(2015-12-01), 329-341  |x 0168-2563  |q 126:3<329  |1 2015  |2 126  |o 10533