caa a22 4500 605517703 CHVBK 20210128100721.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10533-015-0138-8 doi (NATIONALLICENCE)springer-10.1007/s10533-015-0138-8 NanoSIMS investigation of glycine-derived C and N retention with soil organo-mineral associations [Elektronische Daten] [Pierre-Joseph Hatton, Laurent Remusat, Bernd Zeller, Elizabeth Brewer, Delphine Derrien] While microbial-mineral-organic matter interactions are key features controlling the fates of low molecular-weight compounds in soils, direct investigations of how they control their fine-scale spatial distribution are scant. Here, we addressed how microbial transformations affect the retention of 13C/15N-labeled glycine in a forest topsoil 8h after application. We assessed the contribution of soil microorganisms to glycine-derived 13C and 15N retention using γ-irradiated and non-irradiated soils. We tracked down the glycine-derived 13C and 15N at the surface of particles randomly isolated from soil density fractions using nano-scale secondary ions mass spectrometry (NanoSIMS) imaging. Eight hours after addition, 7% of the glycine-derived 13C and 15N initially applied was recovered among soil density fractions, mainly via the activity of soil microorganisms (>85% of total retention). Glycine-derived 13C and 15N distribution among density fractions was correlated with that of soil organic matter (SOM) determined by NanoSIMS (R≥0.85), suggesting that the spatial patterns of the mineral-attached SOM controls the spatial distribution of the glycine-derived 13C and 15N. NanoSIMS images showed largely decoupled glycine-derived 13C and 15N spots preferentially attached to aggregated particles. We speculate that the glycine-derived 13C was principally found within or in the vicinity of microbial cells, whereas the glycine-derived 15N was mostly found as NH4 + and/or exoenzymes spread across soil surfaces. The C:N ratios determined by NanoSIMS suggest that local chemical properties of mineral-attached SOM drive glycine-derived 15N attachment, with the preferential attachment to mineral-attached SOM rich in N (C:N ratios mostly <16). Few exceptions were found in presence of Al and Fe (hydr)oxides (>2.65gcm−3). Springer International Publishing Switzerland, 2015 Soil nationallicence Density nationallicence NanoSIMS nationallicence Glycine nationallicence Biotic nationallicence Retention nationallicence Hatton Pierre-Joseph INRA-Nancy, Biogéochimie des Écosystèmes Forestiers, UR1138, 54280, Champenoux, France aut Remusat Laurent Institut de Minéralogie, de Physique des Matériaux, et de Cosmochimie. UMR CNRS 7590, Sorbonne Universités, Muséum National d'Histoire Naturelle, UPMC, IRD, 61 rue Buffon, 75005, Paris, France aut Zeller Bernd INRA-Nancy, Biogéochimie des Écosystèmes Forestiers, UR1138, 54280, Champenoux, France aut Brewer Elizabeth Department of Crop and Soil Science, Oregon State University, 97331, Corvallis, OR, USA aut Derrien Delphine INRA-Nancy, Biogéochimie des Écosystèmes Forestiers, UR1138, 54280, Champenoux, France aut Biogeochemistry Springer International Publishing 125/3(2015-09-01), 303-313 0168-2563 125:3<303 2015 125 10533 https://doi.org/10.1007/s10533-015-0138-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10533-015-0138-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hatton Pierre-Joseph INRA-Nancy, Biogéochimie des Écosystèmes Forestiers, UR1138, 54280, Champenoux, France aut NATIONALLICENCE 700 1- Remusat Laurent Institut de Minéralogie, de Physique des Matériaux, et de Cosmochimie. UMR CNRS 7590, Sorbonne Universités, Muséum National d'Histoire Naturelle, UPMC, IRD, 61 rue Buffon, 75005, Paris, France aut NATIONALLICENCE 700 1- Zeller Bernd INRA-Nancy, Biogéochimie des Écosystèmes Forestiers, UR1138, 54280, Champenoux, France aut NATIONALLICENCE 700 1- Brewer Elizabeth Department of Crop and Soil Science, Oregon State University, 97331, Corvallis, OR, USA aut NATIONALLICENCE 700 1- Derrien Delphine INRA-Nancy, Biogéochimie des Écosystèmes Forestiers, UR1138, 54280, Champenoux, France aut NATIONALLICENCE 773 0- Biogeochemistry Springer International Publishing 125/3(2015-09-01), 303-313 0168-2563 125:3<303 2015 125 10533