pH measurement of tubular vacuoles of an arbuscular mycorrhizal fungus, Gigaspora margarita
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| 008 | 210128e20150101xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00572-014-0588-1 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00572-014-0588-1 | ||
| 245 | 0 | 0 | |a pH measurement of tubular vacuoles of an arbuscular mycorrhizal fungus, Gigaspora margarita |h [Elektronische Daten] |c [Rintaro Funamoto, Katsuharu Saito, Hiroshi Oyaizu, Toshihiro Aono, Masanori Saito] |
| 520 | 3 | |a Arbuscular mycorrhizal fungi play an important role in phosphate supply to the host plants. The fungal hyphae contain tubular vacuoles where phosphate compounds such as polyphosphate are accumulated. Despite their importance for the phosphate storage, little is known about the physiological properties of the tubular vacuoles in arbuscular mycorrhizal fungi. As an indicator of the physiological state in vacuoles, we measured pH of tubular vacuoles in living hyphae of arbuscular mycorrhizal fungus Gigaspora margarita using ratio image analysis with pH-dependent fluorescent probe, 6-carboxyfluorescein. Fluorescent images of the fine tubular vacuoles were obtained using a laser scanning confocal microscope, which enabled calculation of vacuolar pH with high spatial resolution. The tubular vacuoles showed mean pH of 5.6 and a pH range of 5.1-6.3. These results suggest that the tubular vacuoles of arbuscular mycorrhizal fungi have a mildly acidic pH just like vacuoles of other fungal species including yeast and ectomycorrhizal fungi. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a 6-Carboxyfluorescein diacetate |2 nationallicence | |
| 690 | 7 | |a Arbuscular mycorrhizal symbiosis |2 nationallicence | |
| 690 | 7 | |a Laser scanning confocal microscopy |2 nationallicence | |
| 690 | 7 | |a Tubular vacuole |2 nationallicence | |
| 690 | 7 | |a Vacuolar pH |2 nationallicence | |
| 700 | 1 | |a Funamoto |D Rintaro |u Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, 113-8657, Bunkyo, Tokyo, Japan |4 aut | |
| 700 | 1 | |a Saito |D Katsuharu |u Faculty of Agriculture, Shinshu University, 8304 Minami-minowa, 399-4598, Nagano, Japan |4 aut | |
| 700 | 1 | |a Oyaizu |D Hiroshi |u Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, 113-8657, Bunkyo, Tokyo, Japan |4 aut | |
| 700 | 1 | |a Aono |D Toshihiro |u Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, 113-8657, Bunkyo, Tokyo, Japan |4 aut | |
| 700 | 1 | |a Saito |D Masanori |u Graduate School of Agricultural Science, Tohoku University, 1-1 Amamiya, Tsutsumidori, Aoba, 981-8555, Sendai, Miyagi, Japan |4 aut | |
| 773 | 0 | |t Mycorrhiza |d Springer Berlin Heidelberg |g 25/1(2015-01-01), 55-60 |x 0940-6360 |q 25:1<55 |1 2015 |2 25 |o 572 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00572-014-0588-1 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00572-014-0588-1 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Funamoto |D Rintaro |u Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, 113-8657, Bunkyo, Tokyo, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Saito |D Katsuharu |u Faculty of Agriculture, Shinshu University, 8304 Minami-minowa, 399-4598, Nagano, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Oyaizu |D Hiroshi |u Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, 113-8657, Bunkyo, Tokyo, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Aono |D Toshihiro |u Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, 113-8657, Bunkyo, Tokyo, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Saito |D Masanori |u Graduate School of Agricultural Science, Tohoku University, 1-1 Amamiya, Tsutsumidori, Aoba, 981-8555, Sendai, Miyagi, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Mycorrhiza |d Springer Berlin Heidelberg |g 25/1(2015-01-01), 55-60 |x 0940-6360 |q 25:1<55 |1 2015 |2 25 |o 572 | ||