caa a22 4500 605517827 CHVBK 20210128100722.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00572-014-0588-1 doi (NATIONALLICENCE)springer-10.1007/s00572-014-0588-1 pH measurement of tubular vacuoles of an arbuscular mycorrhizal fungus, Gigaspora margarita [Elektronische Daten] [Rintaro Funamoto, Katsuharu Saito, Hiroshi Oyaizu, Toshihiro Aono, Masanori Saito] Arbuscular mycorrhizal fungi play an important role in phosphate supply to the host plants. The fungal hyphae contain tubular vacuoles where phosphate compounds such as polyphosphate are accumulated. Despite their importance for the phosphate storage, little is known about the physiological properties of the tubular vacuoles in arbuscular mycorrhizal fungi. As an indicator of the physiological state in vacuoles, we measured pH of tubular vacuoles in living hyphae of arbuscular mycorrhizal fungus Gigaspora margarita using ratio image analysis with pH-dependent fluorescent probe, 6-carboxyfluorescein. Fluorescent images of the fine tubular vacuoles were obtained using a laser scanning confocal microscope, which enabled calculation of vacuolar pH with high spatial resolution. The tubular vacuoles showed mean pH of 5.6 and a pH range of 5.1-6.3. These results suggest that the tubular vacuoles of arbuscular mycorrhizal fungi have a mildly acidic pH just like vacuoles of other fungal species including yeast and ectomycorrhizal fungi. Springer-Verlag Berlin Heidelberg, 2014 6-Carboxyfluorescein diacetate nationallicence Arbuscular mycorrhizal symbiosis nationallicence Laser scanning confocal microscopy nationallicence Tubular vacuole nationallicence Vacuolar pH nationallicence Funamoto Rintaro Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, 113-8657, Bunkyo, Tokyo, Japan aut Saito Katsuharu Faculty of Agriculture, Shinshu University, 8304 Minami-minowa, 399-4598, Nagano, Japan aut Oyaizu Hiroshi Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, 113-8657, Bunkyo, Tokyo, Japan aut Aono Toshihiro Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, 113-8657, Bunkyo, Tokyo, Japan aut Saito Masanori Graduate School of Agricultural Science, Tohoku University, 1-1 Amamiya, Tsutsumidori, Aoba, 981-8555, Sendai, Miyagi, Japan aut Mycorrhiza Springer Berlin Heidelberg 25/1(2015-01-01), 55-60 0940-6360 25:1<55 2015 25 572 https://doi.org/10.1007/s00572-014-0588-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00572-014-0588-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Funamoto Rintaro Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, 113-8657, Bunkyo, Tokyo, Japan aut NATIONALLICENCE 700 1- Saito Katsuharu Faculty of Agriculture, Shinshu University, 8304 Minami-minowa, 399-4598, Nagano, Japan aut NATIONALLICENCE 700 1- Oyaizu Hiroshi Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, 113-8657, Bunkyo, Tokyo, Japan aut NATIONALLICENCE 700 1- Aono Toshihiro Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, 113-8657, Bunkyo, Tokyo, Japan aut NATIONALLICENCE 700 1- Saito Masanori Graduate School of Agricultural Science, Tohoku University, 1-1 Amamiya, Tsutsumidori, Aoba, 981-8555, Sendai, Miyagi, Japan aut NATIONALLICENCE 773 0- Mycorrhiza Springer Berlin Heidelberg 25/1(2015-01-01), 55-60 0940-6360 25:1<55 2015 25 572