caa a22 4500 605517932 CHVBK 20210128100722.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00572-014-0616-1 doi (NATIONALLICENCE)springer-10.1007/s00572-014-0616-1 Characterization and colonization of endomycorrhizal Rhizoctonia fungi in the medicinal herb Anoectochilus formosanus (Orchidaceae) [Elektronische Daten] [Jr-Hau Jiang, Yung-I Lee, Marc Cubeta, Lung-Chung Chen] The medicinal effects and techniques for cultivating Anoectochilus formosanus are well-documented, but little is known about the mycorrhizal fungi associated with A. formosanus. Rhizoctonia (Thanatephorus) anastomosis group 6 (AG-6) was the most common species isolated from fungal pelotons in native A. formosanus and represented 67% of the sample. Rhizoctonia (Ceratobasidium) AG-G, P, and R were also isolated and represent the first occurrence in the Orchidaceae. Isolates of AG-6, AG-R, and AG-P in clade I increased seed germination 44-91% and promoted protocorm growth from phases III to VI compared to asymbiotic treatments and isolates of AG-G in clade II and Tulasnella species in clade III. All isolates in clades I to III formed fungal pelotons in tissue-cultured seedlings of A. formosanus, which exhibited significantly greater growth than nonmycorrhizal seedlings. An analysis of the relative effect of treatment ( p ^ i $$ {\widehat{p}}_i $$ ) showed that the low level of colonization ( p ^ i = 0.30 - 0.47 $$ {\widehat{p}}_i = 0.30\hbox{--} 0.47 $$ ) by isolates in clade I resulted in a significant increase in seedling growth compared to isolates in clades II (0.63-0.82) and III (0.63-0.75). There was also a negative correlation (r = −0.8801) with fresh plant weight and fungal colonization. Our results suggest that isolates in clade I may represent an important group associated with native populations of A. formosanus and can vary in their ability to establish a symbiotic association with A. formosanus. The results presented here are potentially useful for advancing research on the medicinal properties, production, and conservation of A. formosanus in diverse ecosystems. The Author(s), 2015 Orchid mycorrhizae nationallicence Rhizoctonia nationallicence Anastomosis group nationallicence Symbiotic germination nationallicence Tissue-cultured seedling nationallicence Jiang Jr-Hau Department of Plant Pathology, National Chung Hsing University, No. 250, Kuo-Kuang Road, Taichung, Taiwan aut Lee Yung-I Botany Department, National Museum of Natural Science, No. 1, Kuan-Chien Road, Taichung, Taiwan aut Cubeta Marc Department of Plant Pathology, Center for Integrated Fungal Research, North Carolina State University, 27695, Raleigh, NC, USA aut Chen Lung-Chung Department of Plant Pathology, National Chung Hsing University, No. 250, Kuo-Kuang Road, Taichung, Taiwan aut Mycorrhiza Springer Berlin Heidelberg 25/6(2015-08-01), 431-445 0940-6360 25:6<431 2015 25 572 https://doi.org/10.1007/s00572-014-0616-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00572-014-0616-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jiang Jr-Hau Department of Plant Pathology, National Chung Hsing University, No. 250, Kuo-Kuang Road, Taichung, Taiwan aut NATIONALLICENCE 700 1- Lee Yung-I Botany Department, National Museum of Natural Science, No. 1, Kuan-Chien Road, Taichung, Taiwan aut NATIONALLICENCE 700 1- Cubeta Marc Department of Plant Pathology, Center for Integrated Fungal Research, North Carolina State University, 27695, Raleigh, NC, USA aut NATIONALLICENCE 700 1- Chen Lung-Chung Department of Plant Pathology, National Chung Hsing University, No. 250, Kuo-Kuang Road, Taichung, Taiwan aut NATIONALLICENCE 773 0- Mycorrhiza Springer Berlin Heidelberg 25/6(2015-08-01), 431-445 0940-6360 25:6<431 2015 25 572