caa a22 4500 605517940 CHVBK 20210128100722.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00572-015-0626-7 doi (NATIONALLICENCE)springer-10.1007/s00572-015-0626-7 A combination of morphology and 28S rRNA gene sequences provide grouping and ranking criteria to merge eight into three Ambispora species ( Ambisporaceae , Glomeromycota ) [Elektronische Daten] [Robert Bills, Joseph Morton] Ambispora, the only genus in Ambisporaceae and one of three deeply rooted families in Archaeosporales, Glomeromycetes, is amended. Analysis of the morphology of specimens from types and living cultures and 28S ribosomal DNA (rDNA; LSU) sequences resulted in two major changes that redefined Ambispora to include only species with the potential for spore dimorphism (acaulosporoid and glomoid). First, species described as producing only glomoid spores (Ambispora leptoticha, Ambispora fecundispora, and Ambispora callosa), only acaulosporoid spores (Ambispora jimgerdemannii), or both spore morphotypes (Ambispora appendicula) were synonymized with a redefined dimorphicspecies, A. leptoticha. LSU sequences and more conserved SSU gene data indicated little divergence between genotypes formerly classified as separate species. Second, Ambispora fennica was synonymized with Ambispora gerdemannii based on morphological and LSU sequence variation equivalent to that measured in the sister clade A. leptoticha. With this analysis, Ambispora was reduced to three species: A. leptoticha, A. gerdemannii, and Ambispora granatensis. Morphological and molecular characters were given equal treatment in this study, as each data set informed and clarified grouping and ranking decisions. The two inner layers of the acaulosporoid spore wall were the only structural characters uniquely defining each of these three species; all other characters were shared. Phenotypes of glomoid spores were indistinguishable between species, and thus were informative only at the genus level. Distinct subclade structure of the LSU gene tree suggests fixation of discrete variants typical of clonal reproduction and possible retention of polymorphisms in rDNA repeats, so that not all discrete geneticvariants are indicative of speciation. Springer-Verlag Berlin Heidelberg, 2015 Arbuscular mycorrhiza nationallicence Dimorphism nationallicence Evolution nationallicence Phylogeny nationallicence Ribosomal DNA nationallicence Synonymization nationallicence Bills Robert Division of Plant and Soil Sciences, West Virginia University, 26506-6108, Morgantown, WV, USA aut Morton Joseph Division of Plant and Soil Sciences, West Virginia University, 26506-6108, Morgantown, WV, USA aut Mycorrhiza Springer Berlin Heidelberg 25/6(2015-08-01), 485-498 0940-6360 25:6<485 2015 25 572 https://doi.org/10.1007/s00572-015-0626-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00572-015-0626-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bills Robert Division of Plant and Soil Sciences, West Virginia University, 26506-6108, Morgantown, WV, USA aut NATIONALLICENCE 700 1- Morton Joseph Division of Plant and Soil Sciences, West Virginia University, 26506-6108, Morgantown, WV, USA aut NATIONALLICENCE 773 0- Mycorrhiza Springer Berlin Heidelberg 25/6(2015-08-01), 485-498 0940-6360 25:6<485 2015 25 572