caa a22 4500 605518033 CHVBK 20210128100723.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00572-014-0602-7 doi (NATIONALLICENCE)springer-10.1007/s00572-014-0602-7 Quantification of arbuscular mycorrhizal fungal DNA in roots: how important is material preservation? [Elektronische Daten] [Martina Janoušková, David Püschel, Martina Hujslová, Renata Slavíková, Jan Jansa] Monitoring populations of arbuscular mycorrhizal fungi (AMF) in roots is a pre-requisite for improving our understanding of AMF ecology and functioning of the symbiosis in natural conditions. Among other approaches, quantification of fungal DNA in plant tissues by quantitative real-time PCR is one of the advanced techniques with a great potential to process large numbers of samples and to deliver truly quantitative information. Its application potential would greatly increase if the samples could be preserved by drying, but little is currently known about the feasibility and reliability of fungal DNA quantification from dry plant material. We addressed this question by comparing quantification results based on dry root material to those obtained from deep-frozen roots of Medicago truncatula colonized with Rhizophagus sp. The fungal DNA was well conserved in the dry root samples with overall fungal DNA levels in the extracts comparable with those determined in extracts of frozen roots. There was, however, no correlation between the quantitative data sets obtained from the two types of material, and data from dry roots were more variable. Based on these results, we recommend dry material for qualitative screenings but advocate using frozen root materials if precise quantification of fungal DNA is required. Springer-Verlag Berlin Heidelberg, 2014 Arbuscular mycorrhizal fungi nationallicence Intraradical colonization nationallicence PCR inhibition nationallicence Quantitative real-time PCR (qPCR) nationallicence Sample preservation nationallicence Janoušková Martina Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Praha 4 - Krč, Czech Republic aut Püschel David Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Praha 4 - Krč, Czech Republic aut Hujslová Martina Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Praha 4 - Krč, Czech Republic aut Slavíková Renata Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Praha 4 - Krč, Czech Republic aut Jansa Jan Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Praha 4 - Krč, Czech Republic aut Mycorrhiza Springer Berlin Heidelberg 25/3(2015-04-01), 205-214 0940-6360 25:3<205 2015 25 572 https://doi.org/10.1007/s00572-014-0602-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00572-014-0602-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Janoušková Martina Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Praha 4 - Krč, Czech Republic aut NATIONALLICENCE 700 1- Püschel David Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Praha 4 - Krč, Czech Republic aut NATIONALLICENCE 700 1- Hujslová Martina Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Praha 4 - Krč, Czech Republic aut NATIONALLICENCE 700 1- Slavíková Renata Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Praha 4 - Krč, Czech Republic aut NATIONALLICENCE 700 1- Jansa Jan Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Praha 4 - Krč, Czech Republic aut NATIONALLICENCE 773 0- Mycorrhiza Springer Berlin Heidelberg 25/3(2015-04-01), 205-214 0940-6360 25:3<205 2015 25 572