caa a22 4500 605518092 CHVBK 20210128100723.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00572-014-0610-7 doi (NATIONALLICENCE)springer-10.1007/s00572-014-0610-7 Do fungicides used to control Rhizoctonia solani impact the non-target arbuscular mycorrhizal fungus Rhizophagus irregularis ? [Elektronische Daten] [Catherine Buysens, Hervé Dupré de Boulois, Stéphane Declerck] There is growing evidence that the application of biocontrol organisms (e.g., Pseudomonas and Bacillus spp., arbuscular mycorrhizal fungi—AMF) is a feasible option to reduce incidence of plant pathogens in an integrated control strategy. However, the utilization of these microorganisms, in particular AMF, may be threatened by the application of fungicides, a widely-used measure to control Rhizoctonia solani in various crops among which potato. Prior to their application, it is thus important to determine the impact of fungicides on AMF. The present study investigated, under in vitro controlled conditions, the impact of azoxystrobin (a systemic broad-spectrum fungicide), flutolanil (a systemic Basidiomycota-specific fungicide), and pencycuron (a contact Rhizoctonia-specific fungicide) and their respective formulations (Amistar, Monarch, and Monceren) on the growth and development of the AMF Rhizophagus irregularis MUCL 41833 (spore germination, root colonization, extraradical mycelium development, and spore production) at doses used to control R. solani. Results demonstrated that azoxystrobin and its formulation Amistar, at threshold values for R. solani control (estimated by the half maximal inhibitory concentration, IC50, on a dry weight basis), did not affect spore germination and potato root colonization by R. irregularis, while the development of extra-radical mycelium and spore production was reduced at 10 times the threshold value. Flutolanil and its formulation Monarch at threshold value did not affect spore germination or extra-radical development but decreased root colonization and arbuscule formation. At threshold value, pencycuron and its formulation Monceren, did not affect spore germination and intra- or extraradical development of R. irregularis. These results suggest that azoxystrobin and pencycuron do not affect the AMF at threshold concentrations to control R. solani in vitro, while flutolanil (as formulation) impacts the intraradical phase of the fungus. These fungicides and R. irregularis thus have the potential to be used in parallel against Rhizoctonia disease in potato. Springer-Verlag Berlin Heidelberg, 2014 Arbuscular mycorrhizal fungi nationallicence Rhizophagus irregularis nationallicence Azoxystrobin nationallicence Flutolanil nationallicence Pencycuron nationallicence Rhizoctonia solani nationallicence Buysens Catherine Earth and Life Institute, Applied Microbiology, Mycology, Université Catholique de Louvain, Croix du Sud, 2 box L7.05.06, 1348, Louvain-la-Neuve, Belgium aut Dupré de Boulois Hervé Earth and Life Institute, Applied Microbiology, Mycology, Université Catholique de Louvain, Croix du Sud, 2 box L7.05.06, 1348, Louvain-la-Neuve, Belgium aut Declerck Stéphane Earth and Life Institute, Applied Microbiology, Mycology, Université Catholique de Louvain, Croix du Sud, 2 box L7.05.06, 1348, Louvain-la-Neuve, Belgium aut Mycorrhiza Springer Berlin Heidelberg 25/4(2015-05-01), 277-288 0940-6360 25:4<277 2015 25 572 https://doi.org/10.1007/s00572-014-0610-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00572-014-0610-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Buysens Catherine Earth and Life Institute, Applied Microbiology, Mycology, Université Catholique de Louvain, Croix du Sud, 2 box L7.05.06, 1348, Louvain-la-Neuve, Belgium aut NATIONALLICENCE 700 1- Dupré de Boulois Hervé Earth and Life Institute, Applied Microbiology, Mycology, Université Catholique de Louvain, Croix du Sud, 2 box L7.05.06, 1348, Louvain-la-Neuve, Belgium aut NATIONALLICENCE 700 1- Declerck Stéphane Earth and Life Institute, Applied Microbiology, Mycology, Université Catholique de Louvain, Croix du Sud, 2 box L7.05.06, 1348, Louvain-la-Neuve, Belgium aut NATIONALLICENCE 773 0- Mycorrhiza Springer Berlin Heidelberg 25/4(2015-05-01), 277-288 0940-6360 25:4<277 2015 25 572