Development of growth media for solid substrate propagation of ectomycorrhizal fungi for inoculation of Norway spruce ( Picea abies ) seedlings
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| 024 | 7 | 0 | |a 10.1007/s00572-014-0611-6 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00572-014-0611-6 | ||
| 245 | 0 | 0 | |a Development of growth media for solid substrate propagation of ectomycorrhizal fungi for inoculation of Norway spruce ( Picea abies ) seedlings |h [Elektronische Daten] |c [Irmeli Vuorinen, Leena Hamberg, Michael Müller, Pekka Seiskari, Taina Pennanen] |
| 520 | 3 | |a A silica-based propagation medium was developed for large-scale production of ectomycorrhizal (ECM) fungal inoculum by solid state fermentation. Development of the medium was started by screening for an optimal growth medium among six different semisynthetic agar media traditionally used in cultivation of ECM fungi. The majority (65%) of the twenty tested ECM fungal strains that typically colonize Norway spruce (Picea abies) seedlings grew best on modified Melin-Norkrans (MMN) medium with reduced sugar content (½MMN). In order to develop a nutritionally similar medium for large-scale cultivation of the ECM fungi, we chose silica to form a solid matrix and light brewery malt extract to provide nutrients. The medium was supplemented with a commercial humic acid product that was shown to boost fungal growth. The optimal concentration of the constituents was screened for in two assays by determining the growth rates of seven potential inoculant ECM fungal strains (Amphinema sp., Cenococcum geophilum, Hebeloma sp., Meliniomyces bicolor, Paxillus involutus, Piloderma byssinum, and Tylospora asterophora). As a result, we composed a silica-based mass propagation medium (pH5.8) containing 2.5% brewery malt extract and 0.5g/l humic acid product Lignohumate AM. This medium is easily produced and supported good growth of even the slowly growing and rarely studied Athelioid ECM strains. Furthermore, root systems of Norway spruce nursery seedlings were colonized by the tested ECM fungi by using solid inoculum formulated from the silica medium. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Ectomycorrhizal fungi |2 nationallicence | |
| 690 | 7 | |a Norway spruce |2 nationallicence | |
| 690 | 7 | |a Picea abies |2 nationallicence | |
| 690 | 7 | |a Fungal inoculum |2 nationallicence | |
| 690 | 7 | |a Solid state fermentation |2 nationallicence | |
| 690 | 7 | |a Malt extract |2 nationallicence | |
| 690 | 7 | |a Humic acid |2 nationallicence | |
| 700 | 1 | |a Vuorinen |D Irmeli |u Finnish Forest Research Institute—Metla, Jokiniemenkuja 1, Box 18, 01301, Vantaa, Finland |4 aut | |
| 700 | 1 | |a Hamberg |D Leena |u Finnish Forest Research Institute—Metla, Jokiniemenkuja 1, Box 18, 01301, Vantaa, Finland |4 aut | |
| 700 | 1 | |a Müller |D Michael |u Finnish Forest Research Institute—Metla, Jokiniemenkuja 1, Box 18, 01301, Vantaa, Finland |4 aut | |
| 700 | 1 | |a Seiskari |D Pekka |u Verdera Ltd., Kurjenkellontie 5 B, Box 5, 02270, Espoo, Finland |4 aut | |
| 700 | 1 | |a Pennanen |D Taina |u Finnish Forest Research Institute—Metla, Jokiniemenkuja 1, Box 18, 01301, Vantaa, Finland |4 aut | |
| 773 | 0 | |t Mycorrhiza |d Springer Berlin Heidelberg |g 25/4(2015-05-01), 311-324 |x 0940-6360 |q 25:4<311 |1 2015 |2 25 |o 572 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00572-014-0611-6 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00572-014-0611-6 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Vuorinen |D Irmeli |u Finnish Forest Research Institute—Metla, Jokiniemenkuja 1, Box 18, 01301, Vantaa, Finland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Hamberg |D Leena |u Finnish Forest Research Institute—Metla, Jokiniemenkuja 1, Box 18, 01301, Vantaa, Finland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Müller |D Michael |u Finnish Forest Research Institute—Metla, Jokiniemenkuja 1, Box 18, 01301, Vantaa, Finland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Seiskari |D Pekka |u Verdera Ltd., Kurjenkellontie 5 B, Box 5, 02270, Espoo, Finland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Pennanen |D Taina |u Finnish Forest Research Institute—Metla, Jokiniemenkuja 1, Box 18, 01301, Vantaa, Finland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Mycorrhiza |d Springer Berlin Heidelberg |g 25/4(2015-05-01), 311-324 |x 0940-6360 |q 25:4<311 |1 2015 |2 25 |o 572 | ||