caa a22 4500 605518106 CHVBK 20210128100723.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00572-014-0611-6 doi (NATIONALLICENCE)springer-10.1007/s00572-014-0611-6 Development of growth media for solid substrate propagation of ectomycorrhizal fungi for inoculation of Norway spruce ( Picea abies ) seedlings [Elektronische Daten] [Irmeli Vuorinen, Leena Hamberg, Michael Müller, Pekka Seiskari, Taina Pennanen] A silica-based propagation medium was developed for large-scale production of ectomycorrhizal (ECM) fungal inoculum by solid state fermentation. Development of the medium was started by screening for an optimal growth medium among six different semisynthetic agar media traditionally used in cultivation of ECM fungi. The majority (65%) of the twenty tested ECM fungal strains that typically colonize Norway spruce (Picea abies) seedlings grew best on modified Melin-Norkrans (MMN) medium with reduced sugar content (½MMN). In order to develop a nutritionally similar medium for large-scale cultivation of the ECM fungi, we chose silica to form a solid matrix and light brewery malt extract to provide nutrients. The medium was supplemented with a commercial humic acid product that was shown to boost fungal growth. The optimal concentration of the constituents was screened for in two assays by determining the growth rates of seven potential inoculant ECM fungal strains (Amphinema sp., Cenococcum geophilum, Hebeloma sp., Meliniomyces bicolor, Paxillus involutus, Piloderma byssinum, and Tylospora asterophora). As a result, we composed a silica-based mass propagation medium (pH5.8) containing 2.5% brewery malt extract and 0.5g/l humic acid product Lignohumate AM. This medium is easily produced and supported good growth of even the slowly growing and rarely studied Athelioid ECM strains. Furthermore, root systems of Norway spruce nursery seedlings were colonized by the tested ECM fungi by using solid inoculum formulated from the silica medium. Springer-Verlag Berlin Heidelberg, 2014 Ectomycorrhizal fungi nationallicence Norway spruce nationallicence Picea abies nationallicence Fungal inoculum nationallicence Solid state fermentation nationallicence Malt extract nationallicence Humic acid nationallicence Vuorinen Irmeli Finnish Forest Research Institute—Metla, Jokiniemenkuja 1, Box 18, 01301, Vantaa, Finland aut Hamberg Leena Finnish Forest Research Institute—Metla, Jokiniemenkuja 1, Box 18, 01301, Vantaa, Finland aut Müller Michael Finnish Forest Research Institute—Metla, Jokiniemenkuja 1, Box 18, 01301, Vantaa, Finland aut Seiskari Pekka Verdera Ltd., Kurjenkellontie 5 B, Box 5, 02270, Espoo, Finland aut Pennanen Taina Finnish Forest Research Institute—Metla, Jokiniemenkuja 1, Box 18, 01301, Vantaa, Finland aut Mycorrhiza Springer Berlin Heidelberg 25/4(2015-05-01), 311-324 0940-6360 25:4<311 2015 25 572 https://doi.org/10.1007/s00572-014-0611-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00572-014-0611-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vuorinen Irmeli Finnish Forest Research Institute—Metla, Jokiniemenkuja 1, Box 18, 01301, Vantaa, Finland aut NATIONALLICENCE 700 1- Hamberg Leena Finnish Forest Research Institute—Metla, Jokiniemenkuja 1, Box 18, 01301, Vantaa, Finland aut NATIONALLICENCE 700 1- Müller Michael Finnish Forest Research Institute—Metla, Jokiniemenkuja 1, Box 18, 01301, Vantaa, Finland aut NATIONALLICENCE 700 1- Seiskari Pekka Verdera Ltd., Kurjenkellontie 5 B, Box 5, 02270, Espoo, Finland aut NATIONALLICENCE 700 1- Pennanen Taina Finnish Forest Research Institute—Metla, Jokiniemenkuja 1, Box 18, 01301, Vantaa, Finland aut NATIONALLICENCE 773 0- Mycorrhiza Springer Berlin Heidelberg 25/4(2015-05-01), 311-324 0940-6360 25:4<311 2015 25 572