caa a22 4500 605518173 CHVBK 20210128100724.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00572-015-0640-9 doi (NATIONALLICENCE)springer-10.1007/s00572-015-0640-9 Fertility-dependent effects of ectomycorrhizal fungal communities on white spruce seedling nutrition [Elektronische Daten] [Alistair Smith II, Lynette Potvin, Erik Lilleskov] Ectomycorrhizal fungi (EcMF) typically colonize nursery seedlings, but nutritional and growth effects of these communities are only partly understood. To examine these effects, Picea glauca seedlings collected from a tree nursery naturally colonized by three dominant EcMF were divided between fertilized and unfertilized treatments. After one growing season seedlings were harvested, ectomycorrhizas identified using DNA sequencing, and seedlings analyzed for leaf nutrient concentration and content, and biomass parameters. EcMF community structure-nutrient interactions were tested using nonmetric multidimensional scaling (NMDS) combined with vector analysis of foliar nutrients and biomass. We identified three dominant species: Amphinema sp., Atheliaceae sp., and Thelephora terrestris. NMDS + envfit revealed significant community effects on seedling nutrition that differed with fertilization treatment. PERMANOVA and regression analyses uncovered significant species effects on host nutrient concentration, content, and stoichiometry. Amphinema sp. had a significant positive effect on phosphorus (P), calcium and zinc concentration, and P content; in contrast, T. terrestris had a negative effect on P concentration. In the unfertilized treatment, percent abundance of the Amphinema sp. negatively affected foliar nitrogen (N) concentration but not content, and reduced foliar N/P. In fertilized seedlings, Amphinema sp. was positively related to foliar concentrations of N, magnesium, and boron, and both concentration and content of manganese, and Atheliaceae sp. had a negative relationship with P content. Findings shed light on the community and species effects on seedling condition, revealing clear functional differences among dominants. The approach used should be scalable to explore function in more complex communities composed of unculturable EcMF. Springer-Verlag Berlin Heidelberg (outside the USA), 2015 Stoichiometry nationallicence Ectomycorrhizal fungal community effects nationallicence Nitrogen nationallicence Phosphorus nationallicence Micronutrients nationallicence Amphinema nationallicence Atheliaceae nationallicence Thelephora terrestris nationallicence Greenhouse nationallicence Smith II Alistair Michigan Technological University, 1400 Townsend Dr, 49931, Houghton, MI, USA aut Potvin Lynette Northern Research Station, US Forest Service, 410 MacInnes Drive, 49931-1199, Houghton, MI, USA aut Lilleskov Erik Northern Research Station, US Forest Service, 410 MacInnes Drive, 49931-1199, Houghton, MI, USA aut Mycorrhiza Springer Berlin Heidelberg 25/8(2015-11-01), 649-662 0940-6360 25:8<649 2015 25 572 https://doi.org/10.1007/s00572-015-0640-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00572-015-0640-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Smith II Alistair Michigan Technological University, 1400 Townsend Dr, 49931, Houghton, MI, USA aut NATIONALLICENCE 700 1- Potvin Lynette Northern Research Station, US Forest Service, 410 MacInnes Drive, 49931-1199, Houghton, MI, USA aut NATIONALLICENCE 700 1- Lilleskov Erik Northern Research Station, US Forest Service, 410 MacInnes Drive, 49931-1199, Houghton, MI, USA aut NATIONALLICENCE 773 0- Mycorrhiza Springer Berlin Heidelberg 25/8(2015-11-01), 649-662 0940-6360 25:8<649 2015 25 572