Arbuscular mycorrhiza increase artemisinin accumulation in Artemisia annua by higher expression of key biosynthesis genes via enhanced jasmonic acid levels
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| 024 | 7 | 0 | |a 10.1007/s00572-014-0614-3 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00572-014-0614-3 | ||
| 245 | 0 | 0 | |a Arbuscular mycorrhiza increase artemisinin accumulation in Artemisia annua by higher expression of key biosynthesis genes via enhanced jasmonic acid levels |h [Elektronische Daten] |c [Shantanu Mandal, Shivangi Upadhyay, Saima Wajid, Mauji Ram, Dharam Jain, Ved Singh, Malik Abdin, Rupam Kapoor] |
| 520 | 3 | |a It is becoming increasingly evident that the formation of arbuscular mycorrhiza (AM) enhances secondary metabolite production in shoots. Despite mounting evidence, relatively little is known about the underlying mechanisms. This study suggests that increase in artemisinin concentration in Artemisia annua colonized by Rhizophagus intraradices is due to altered trichome density as well as transcriptional patterns that are mediated via enhanced jasmonic acid (JA) levels. Mycorrhizal (M) plants had higher JA levels in leaf tissue that may be due to induction of an allene oxidase synthase gene (AOS), encoding one of the key enzymes for JA production. Non-mycorrhizal (NM) plants were exogenously supplied with a range of methyl jasmonic acid concentrations. When leaves of NM and M plants with similar levels of endogenous JA were compared, these matched closely in terms of shoot trichome density, artemisinin concentration, and transcript profile of artemisinin biosynthesis genes. Mycorrhization increased artemisinin levels by increasing glandular trichome density and transcriptional activation of artemisinin biosynthesis genes. Transcriptional analysis of some rate-limiting enzymes of mevalonate and methyl erythritol phosphate (MEP) pathways revealed that AM increases isoprenoids by induction of the MEP pathway. A decline in artemisinin concentration in shoots of NM and M plants treated with ibuprofen (an inhibitor of JA biosynthesis) further confirmed the implication of JA in the mechanism of artemisinin production. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Arbuscular mycorrhizal fungi |2 nationallicence | |
| 690 | 7 | |a Jasmonic acid |2 nationallicence | |
| 690 | 7 | |a Trichome density |2 nationallicence | |
| 690 | 7 | |a Artemisinin biosynthesis genes |2 nationallicence | |
| 690 | 7 | |a Methyl erythritol phosphate pathway |2 nationallicence | |
| 690 | 7 | |a Mevalonate pathway |2 nationallicence | |
| 700 | 1 | |a Mandal |D Shantanu |u Department of Botany, University of Delhi, 110007, Delhi, India |4 aut | |
| 700 | 1 | |a Upadhyay |D Shivangi |u Department of Botany, University of Delhi, 110007, Delhi, India |4 aut | |
| 700 | 1 | |a Wajid |D Saima |u Department of Biotechnology, Centre for Transgenic Plant Development, 110062, Jamia Hamdard, New Delhi, India |4 aut | |
| 700 | 1 | |a Ram |D Mauji |u Department of Green Technology, Ipca Laboratories Ltd., 457002, Ratlam, Madhya Pradesh, India |4 aut | |
| 700 | 1 | |a Jain |D Dharam |u Department of Green Technology, Ipca Laboratories Ltd., 457002, Ratlam, Madhya Pradesh, India |4 aut | |
| 700 | 1 | |a Singh |D Ved |u Department of Botany, University of Delhi, 110007, Delhi, India |4 aut | |
| 700 | 1 | |a Abdin |D Malik |u Department of Biotechnology, Centre for Transgenic Plant Development, 110062, Jamia Hamdard, New Delhi, India |4 aut | |
| 700 | 1 | |a Kapoor |D Rupam |u Department of Botany, University of Delhi, 110007, Delhi, India |4 aut | |
| 773 | 0 | |t Mycorrhiza |d Springer Berlin Heidelberg |g 25/5(2015-07-01), 345-357 |x 0940-6360 |q 25:5<345 |1 2015 |2 25 |o 572 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00572-014-0614-3 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00572-014-0614-3 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Mandal |D Shantanu |u Department of Botany, University of Delhi, 110007, Delhi, India |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Upadhyay |D Shivangi |u Department of Botany, University of Delhi, 110007, Delhi, India |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wajid |D Saima |u Department of Biotechnology, Centre for Transgenic Plant Development, 110062, Jamia Hamdard, New Delhi, India |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ram |D Mauji |u Department of Green Technology, Ipca Laboratories Ltd., 457002, Ratlam, Madhya Pradesh, India |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Jain |D Dharam |u Department of Green Technology, Ipca Laboratories Ltd., 457002, Ratlam, Madhya Pradesh, India |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Singh |D Ved |u Department of Botany, University of Delhi, 110007, Delhi, India |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Abdin |D Malik |u Department of Biotechnology, Centre for Transgenic Plant Development, 110062, Jamia Hamdard, New Delhi, India |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kapoor |D Rupam |u Department of Botany, University of Delhi, 110007, Delhi, India |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Mycorrhiza |d Springer Berlin Heidelberg |g 25/5(2015-07-01), 345-357 |x 0940-6360 |q 25:5<345 |1 2015 |2 25 |o 572 | ||