caa a22 4500 605518246 CHVBK 20210128100724.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00572-014-0614-3 doi (NATIONALLICENCE)springer-10.1007/s00572-014-0614-3 Arbuscular mycorrhiza increase artemisinin accumulation in Artemisia annua by higher expression of key biosynthesis genes via enhanced jasmonic acid levels [Elektronische Daten] [Shantanu Mandal, Shivangi Upadhyay, Saima Wajid, Mauji Ram, Dharam Jain, Ved Singh, Malik Abdin, Rupam Kapoor] It is becoming increasingly evident that the formation of arbuscular mycorrhiza (AM) enhances secondary metabolite production in shoots. Despite mounting evidence, relatively little is known about the underlying mechanisms. This study suggests that increase in artemisinin concentration in Artemisia annua colonized by Rhizophagus intraradices is due to altered trichome density as well as transcriptional patterns that are mediated via enhanced jasmonic acid (JA) levels. Mycorrhizal (M) plants had higher JA levels in leaf tissue that may be due to induction of an allene oxidase synthase gene (AOS), encoding one of the key enzymes for JA production. Non-mycorrhizal (NM) plants were exogenously supplied with a range of methyl jasmonic acid concentrations. When leaves of NM and M plants with similar levels of endogenous JA were compared, these matched closely in terms of shoot trichome density, artemisinin concentration, and transcript profile of artemisinin biosynthesis genes. Mycorrhization increased artemisinin levels by increasing glandular trichome density and transcriptional activation of artemisinin biosynthesis genes. Transcriptional analysis of some rate-limiting enzymes of mevalonate and methyl erythritol phosphate (MEP) pathways revealed that AM increases isoprenoids by induction of the MEP pathway. A decline in artemisinin concentration in shoots of NM and M plants treated with ibuprofen (an inhibitor of JA biosynthesis) further confirmed the implication of JA in the mechanism of artemisinin production. Springer-Verlag Berlin Heidelberg, 2014 Arbuscular mycorrhizal fungi nationallicence Jasmonic acid nationallicence Trichome density nationallicence Artemisinin biosynthesis genes nationallicence Methyl erythritol phosphate pathway nationallicence Mevalonate pathway nationallicence Mandal Shantanu Department of Botany, University of Delhi, 110007, Delhi, India aut Upadhyay Shivangi Department of Botany, University of Delhi, 110007, Delhi, India aut Wajid Saima Department of Biotechnology, Centre for Transgenic Plant Development, 110062, Jamia Hamdard, New Delhi, India aut Ram Mauji Department of Green Technology, Ipca Laboratories Ltd., 457002, Ratlam, Madhya Pradesh, India aut Jain Dharam Department of Green Technology, Ipca Laboratories Ltd., 457002, Ratlam, Madhya Pradesh, India aut Singh Ved Department of Botany, University of Delhi, 110007, Delhi, India aut Abdin Malik Department of Biotechnology, Centre for Transgenic Plant Development, 110062, Jamia Hamdard, New Delhi, India aut Kapoor Rupam Department of Botany, University of Delhi, 110007, Delhi, India aut Mycorrhiza Springer Berlin Heidelberg 25/5(2015-07-01), 345-357 0940-6360 25:5<345 2015 25 572 https://doi.org/10.1007/s00572-014-0614-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00572-014-0614-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mandal Shantanu Department of Botany, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Upadhyay Shivangi Department of Botany, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Wajid Saima Department of Biotechnology, Centre for Transgenic Plant Development, 110062, Jamia Hamdard, New Delhi, India aut NATIONALLICENCE 700 1- Ram Mauji Department of Green Technology, Ipca Laboratories Ltd., 457002, Ratlam, Madhya Pradesh, India aut NATIONALLICENCE 700 1- Jain Dharam Department of Green Technology, Ipca Laboratories Ltd., 457002, Ratlam, Madhya Pradesh, India aut NATIONALLICENCE 700 1- Singh Ved Department of Botany, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Abdin Malik Department of Biotechnology, Centre for Transgenic Plant Development, 110062, Jamia Hamdard, New Delhi, India aut NATIONALLICENCE 700 1- Kapoor Rupam Department of Botany, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 773 0- Mycorrhiza Springer Berlin Heidelberg 25/5(2015-07-01), 345-357 0940-6360 25:5<345 2015 25 572