caa a22 4500 605518297 CHVBK 20210128100724.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00572-014-0623-2 doi (NATIONALLICENCE)springer-10.1007/s00572-014-0623-2 Up-regulation of genes involved in N -acetylglucosamine uptake and metabolism suggests a recycling mode of chitin in intraradical mycelium of arbuscular mycorrhizal fungi [Elektronische Daten] [Yoshihiro Kobae, Miki Kawachi, Katsuharu Saito, Yusuke Kikuchi, Tatsuhiro Ezawa, Masayoshi Maeshima, Shingo Hata, Toru Fujiwara] Arbuscular mycorrhizal (AM) fungi colonize roots and form two kinds of mycelium, intraradical mycelium (IRM) and extraradical mycelium (ERM). Arbuscules are characteristic IRM structures that highly branch within host cells in order to mediate resource exchange between the symbionts. They are ephemeral structures and at the end of their life span, arbuscular branches collapse from the tip, fungal cytoplasm withdraws, and the whole arbuscule shrinks into fungal clumps. The exoskeleton of an arbuscule contains structured chitin, which is a polymer of N-acetylglucosamine (GlcNAc), whereas a collapsed arbuscule does not. The molecular mechanisms underlying the turnover of chitin in AM fungi remain unknown. Here, a GlcNAc transporter, RiNGT, was identified from the AM fungus Rhizophagus irregularis. Yeast mutants defective in endogenous GlcNAc uptake and expressing RiNGT took up 14C-GlcNAc, and the optimum uptake was at acidic pH values (pH 4.0-4.5). The transcript levels of RiNGT in IRM in mycorrhizal Lotus japonicus roots were over 1000 times higher than those in ERM. GlcNAc-6-phosphate deacetylase (DAC1) and glucosamine-6-phosphate isomerase (NAG1) genes, which are related to the GlcNAc catabolism pathway, were also induced in IRM. Altogether, data suggest the existence of an enhanced recycling mode of GlcNAc in IRM of AM fungi. Springer-Verlag Berlin Heidelberg, 2015 Arbuscular mycorrhizal fungi nationallicence Chitin recycling nationallicence N -acetylglucosamine (GlcNAc) transporter nationallicence Rhizophagus irregularis nationallicence Kobae Yoshihiro Graduate School of Agricultural and Life Sciences, University of Tokyo, 113-8657, Tokyo, Japan aut Kawachi Miki Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Japan aut Saito Katsuharu Faculty of Agriculture, Shinshu University, 8304 Minami-minowa, 399-4598, Nagano, Japan aut Kikuchi Yusuke Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Hokkaido, Japan aut Ezawa Tatsuhiro Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Hokkaido, Japan aut Maeshima Masayoshi Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Japan aut Hata Shingo Institute of Agriculture, Ryukoku University, 67 Tsukamoto-cho, Fukakusa, 612-8577, Fushimi-ku, Kyoto, Japan aut Fujiwara Toru Graduate School of Agricultural and Life Sciences, University of Tokyo, 113-8657, Tokyo, Japan aut Mycorrhiza Springer Berlin Heidelberg 25/5(2015-07-01), 411-417 0940-6360 25:5<411 2015 25 572 https://doi.org/10.1007/s00572-014-0623-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00572-014-0623-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kobae Yoshihiro Graduate School of Agricultural and Life Sciences, University of Tokyo, 113-8657, Tokyo, Japan aut NATIONALLICENCE 700 1- Kawachi Miki Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Japan aut NATIONALLICENCE 700 1- Saito Katsuharu Faculty of Agriculture, Shinshu University, 8304 Minami-minowa, 399-4598, Nagano, Japan aut NATIONALLICENCE 700 1- Kikuchi Yusuke Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Hokkaido, Japan aut NATIONALLICENCE 700 1- Ezawa Tatsuhiro Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Hokkaido, Japan aut NATIONALLICENCE 700 1- Maeshima Masayoshi Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Japan aut NATIONALLICENCE 700 1- Hata Shingo Institute of Agriculture, Ryukoku University, 67 Tsukamoto-cho, Fukakusa, 612-8577, Fushimi-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Fujiwara Toru Graduate School of Agricultural and Life Sciences, University of Tokyo, 113-8657, Tokyo, Japan aut NATIONALLICENCE 773 0- Mycorrhiza Springer Berlin Heidelberg 25/5(2015-07-01), 411-417 0940-6360 25:5<411 2015 25 572