caa a22 4500 605518351 CHVBK 20210128100725.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00572-014-0598-z doi (NATIONALLICENCE)springer-10.1007/s00572-014-0598-z Mycorrhiza-induced lower oxidative burst is related with higher antioxidant enzyme activities, net H2O2 effluxes, and Ca2+ influxes in trifoliate orange roots under drought stress [Elektronische Daten] [Ying-Ning Zou, Yong-Ming Huang, Qiang-Sheng Wu, Xin-Hua He] Mechanisms of arbuscular mycorrhiza (AM)-induced lower oxidative burst of host plants under drought stress (DS) are not elucidated. A noninvasive microtest technology (NMT) was used to investigate the effects of Funneliformis mosseae on net fluxes of root hydrogen peroxide (H2O2) and calcium ions (Ca2+) in 5-month-old Poncirus trifoliata, in combination with catalase (CAT) and superoxide dismutase (SOD) activities as well as tissue superoxide radical (O2 •−) and H2O2 concentrations under DS and well-watered (WW) conditions. A 2-month DS (55% maximum water holding capacity of growth substrates) significantly inhibited AM fungal root colonization, while AM symbiosis significantly increased plant biomass production, irrespective of water status. F. mosseae inoculation generally increased SOD and CAT activity but decreased O2 •− and H2O2 concentrations in leaves and roots under WW and DS. Compared with non-AM seedlings, roots of AM seedlings had significantly higher net H2O2 effluxes and net Ca2+ influxes, especially in the meristem zone, but lower net H2O2 efflux in the elongation zone. Net Ca2+ influxes into roots were significantly positively correlated with root net H2O2 effluxes but negatively with root H2O2 concentrations. Results from this study suggest that AM-induced lower oxidative burst is related with higher antioxidant enzyme activities, root net H2O2 effluxes, and Ca2+ influxes under WW and DS. Springer-Verlag Berlin Heidelberg, 2014 Arbuscular mycorrhiza nationallicence Calcium nationallicence Citrus nationallicence Hydrogen peroxide nationallicence Noninvasive microtest technology nationallicence Zou Ying-Ning College of Horticulture and Gardening/Institute of Root Biology, Yangtze University, 88 Jingmi Road, 434025, Jingzhou, Hubei, People's Republic of China aut Huang Yong-Ming College of Horticulture and Gardening/Institute of Root Biology, Yangtze University, 88 Jingmi Road, 434025, Jingzhou, Hubei, People's Republic of China aut Wu Qiang-Sheng College of Horticulture and Gardening/Institute of Root Biology, Yangtze University, 88 Jingmi Road, 434025, Jingzhou, Hubei, People's Republic of China aut He Xin-Hua Department of Environmental Sciences, University of Sydney, 2015, Eveleigh, NSW, Australia aut Mycorrhiza Springer Berlin Heidelberg 25/2(2015-02-01), 143-152 0940-6360 25:2<143 2015 25 572 https://doi.org/10.1007/s00572-014-0598-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00572-014-0598-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zou Ying-Ning College of Horticulture and Gardening/Institute of Root Biology, Yangtze University, 88 Jingmi Road, 434025, Jingzhou, Hubei, People's Republic of China aut NATIONALLICENCE 700 1- Huang Yong-Ming College of Horticulture and Gardening/Institute of Root Biology, Yangtze University, 88 Jingmi Road, 434025, Jingzhou, Hubei, People's Republic of China aut NATIONALLICENCE 700 1- Wu Qiang-Sheng College of Horticulture and Gardening/Institute of Root Biology, Yangtze University, 88 Jingmi Road, 434025, Jingzhou, Hubei, People's Republic of China aut NATIONALLICENCE 700 1- He Xin-Hua Department of Environmental Sciences, University of Sydney, 2015, Eveleigh, NSW, Australia aut NATIONALLICENCE 773 0- Mycorrhiza Springer Berlin Heidelberg 25/2(2015-02-01), 143-152 0940-6360 25:2<143 2015 25 572