caa a22 4500 605520461 CHVBK 20210128100735.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00223-015-9989-1 doi (NATIONALLICENCE)springer-10.1007/s00223-015-9989-1 Ectopic Hard Tissue Formation by Odonto/Osteogenically In Vitro Differentiated Human Deciduous Teeth Pulp Stem Cells [Elektronische Daten] [Seunghye Kim, Je Song, Mijeong Jeon, Dong Shin, Seong-Oh Kim, Jae Lee] There have been many attempts to use the pulp tissue from human deciduous teeth for dentin or bone regeneration. The objective of this study was to determine the effects of odonto/osteogenic in vitro differentiation of deciduous teeth pulp stem cells (DTSCs) on their in vivo hard tissue-forming potential. DTSCs were isolated from extracted deciduous teeth using the outgrowth method. These cells were exposed to odonto/osteogenic stimuli for 4 and 8days (Day 4 and Day 8 groups, respectively), while cells in the control group were cultured in normal medium. The in vitro differentiated DTSCs and the control DTSCs were transplanted subcutaneously into immunocompromised mice with macroporous biphasic calcium phosphate and sacrificed at 8weeks post-implantation. The effect of odonto/osteogenic in vitro differentiation was evaluated using alkaline phosphatase (ALP) staining and quantitative reverse transcription polymerase chain reaction (RT-PCR). The in vivo effect was evaluated by qualitative RT-PCR, assessment of ALP activity, histologic analysis, and immunohistochemical staining. The amount of hard tissue was greater in Day 4 group than Day 8 group (p=0.014). However, Day 8 group generated lamellar bone-like structure, which was immunonegative to anti-human dentin sialoprotein with significantly low expression level of DSPP compared with the control group (p=0.008). This study demonstrates that odonto/osteogenic in vitro differentiation of DTSCs enhances the formation of bone-like tissue, instead of dentin-like tissue, when transplanted subcutaneously using MBCP as a carrier. The odonto/osteogenic in vitro differentiation of DTSCs may be an effective modification that enhances in vivo bone formation by DTSCs. Springer Science+Business Media New York, 2015 Deciduous teeth pulp stem cell nationallicence Odonto/osteogenic in vitro differentiation nationallicence Hard tissue nationallicence Kim Seunghye Department of Pediatric Dentistry, College of Dentistry, Yonsei University, 250 Seongsanno, Seodaemun-gu, 120-752, Seoul, Korea aut Song Je Department of Pediatric Dentistry, College of Dentistry, Yonsei University, 250 Seongsanno, Seodaemun-gu, 120-752, Seoul, Korea aut Jeon Mijeong Oral Science Research Center, College of Dentistry, Yonsei University, Seoul, Korea aut Shin Dong Department of Oral Biology, College of Dentistry, Yonsei University, Seoul, Korea aut Kim Seong-Oh Department of Pediatric Dentistry, College of Dentistry, Yonsei University, 250 Seongsanno, Seodaemun-gu, 120-752, Seoul, Korea aut Lee Jae Department of Pediatric Dentistry, College of Dentistry, Yonsei University, 250 Seongsanno, Seodaemun-gu, 120-752, Seoul, Korea aut Calcified Tissue International Springer US; http://www.springer-ny.com 97/1(2015-07-01), 80-89 0171-967X 97:1<80 2015 97 223 https://doi.org/10.1007/s00223-015-9989-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00223-015-9989-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kim Seunghye Department of Pediatric Dentistry, College of Dentistry, Yonsei University, 250 Seongsanno, Seodaemun-gu, 120-752, Seoul, Korea aut NATIONALLICENCE 700 1- Song Je Department of Pediatric Dentistry, College of Dentistry, Yonsei University, 250 Seongsanno, Seodaemun-gu, 120-752, Seoul, Korea aut NATIONALLICENCE 700 1- Jeon Mijeong Oral Science Research Center, College of Dentistry, Yonsei University, Seoul, Korea aut NATIONALLICENCE 700 1- Shin Dong Department of Oral Biology, College of Dentistry, Yonsei University, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Seong-Oh Department of Pediatric Dentistry, College of Dentistry, Yonsei University, 250 Seongsanno, Seodaemun-gu, 120-752, Seoul, Korea aut NATIONALLICENCE 700 1- Lee Jae Department of Pediatric Dentistry, College of Dentistry, Yonsei University, 250 Seongsanno, Seodaemun-gu, 120-752, Seoul, Korea aut NATIONALLICENCE 773 0- Calcified Tissue International Springer US; http://www.springer-ny.com 97/1(2015-07-01), 80-89 0171-967X 97:1<80 2015 97 223