caa a22 4500 605520542 CHVBK 20210128100735.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00223-015-0055-9 doi (NATIONALLICENCE)springer-10.1007/s00223-015-0055-9 Identification and Characterization of a Synthetic Osteogenic Peptide [Elektronische Daten] [David Komatsu, Michael Hadjiargyrou, Sardar Udin, Nicholas Trasolini, Srinivas Pentyala] Osteoporosis is the most common metabolic bone disorder and its management represents a tremendous public health encumbrance. While several classes of therapeutics have been approved to treat this disease, all are associated with significant adverse effects. An algorithm was developed and utilized to discover potential bioactive peptides, which led to the identification of an osteogenic peptide that mapped to the C-terminal region of the calcitonin receptor and has been named calcitonin receptor fragment peptide (CRFP). In vitro treatment of human mesenchymal stem cells with CRFP resulted in dose-specific effects on both proliferation and osteoblastic differentiation. Similarly, in vitro treatment of rat RCJ3.1C5.18 cells led to dose- and species-specific effects on proliferation. A rat ovariectomy (OVX) model was used to assess the potential efficacy of CRFP in treating osteoporosis. MicroCT analysis of distal femoral samples showed that OVX rats treated with CRFP were significantly protected from losses of 55% in trabecular bone volume fraction (BVF), 42% in connectivity density, and 18% in trabecular thickness in comparison to vehicle-treated controls. MicroCT analyses of vertebrae revealed CRFP to significantly prevent a 25% reduction in BVF. MicroCT evaluation of femoral and vertebral cortical bone found a significant reduction of 2% in vertebral bone mineral density. In summary, our in vitro studies indicate that CRFP is both bioactive and osteogenic and our in vivo studies indicate that CRFP is skeletally bioactive. These promising data indicate that further in vitro and in vivo evaluation of CRFP as a new treatment for osteoporosis is warranted. Springer Science+Business Media New York, 2015 Osteoporosis nationallicence MicroCT nationallicence Calcitonin nationallicence Bioinformatics nationallicence Ovariectomy nationallicence Komatsu David Department of Orthopaedics, HSC-T18, Stony Brook University, Room 085, 11794-8181, Stony Brook, NY, USA aut Hadjiargyrou Michael Department of Life Sciences, Theobald Science Center, New York Institute of Technology, Room 420, 11568-8000, Old Westbury, NY, USA aut Udin Sardar Department of Orthopaedics, HSC-T18, Stony Brook University, Room 085, 11794-8181, Stony Brook, NY, USA aut Trasolini Nicholas School of Medicine, HSC-T4, Stony Brook University, Room 147, 11794-8434, Stony Brook, NY, USA aut Pentyala Srinivas Department of Anesthesiology, HSC-L4, Stony Brook University Medical Center, Room 85, 11794-8480, Stony Brook, NY, USA aut Calcified Tissue International Springer US; http://www.springer-ny.com 97/6(2015-12-01), 611-623 0171-967X 97:6<611 2015 97 223 https://doi.org/10.1007/s00223-015-0055-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00223-015-0055-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Komatsu David Department of Orthopaedics, HSC-T18, Stony Brook University, Room 085, 11794-8181, Stony Brook, NY, USA aut NATIONALLICENCE 700 1- Hadjiargyrou Michael Department of Life Sciences, Theobald Science Center, New York Institute of Technology, Room 420, 11568-8000, Old Westbury, NY, USA aut NATIONALLICENCE 700 1- Udin Sardar Department of Orthopaedics, HSC-T18, Stony Brook University, Room 085, 11794-8181, Stony Brook, NY, USA aut NATIONALLICENCE 700 1- Trasolini Nicholas School of Medicine, HSC-T4, Stony Brook University, Room 147, 11794-8434, Stony Brook, NY, USA aut NATIONALLICENCE 700 1- Pentyala Srinivas Department of Anesthesiology, HSC-L4, Stony Brook University Medical Center, Room 85, 11794-8480, Stony Brook, NY, USA aut NATIONALLICENCE 773 0- Calcified Tissue International Springer US; http://www.springer-ny.com 97/6(2015-12-01), 611-623 0171-967X 97:6<611 2015 97 223