caa a22 4500 605520577 CHVBK 20210128100736.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00223-015-0042-1 doi (NATIONALLICENCE)springer-10.1007/s00223-015-0042-1 Age-Related Effects of Advanced Glycation End Products (Ages) in Bone Matrix on Osteoclastic Resorption [Elektronische Daten] [Xiao Yang, Chintan Gandhi, MD. Rahman, Mark Appleford, Lian-Wen Sun, Xiaodu Wang] Advanced glycation end products (AGEs) accumulate in bone extracellular matrix as people age. Previous studies have shown controversial results regarding the role of in situ AGEs accumulation in osteoclastic resorption. To address this issue, this study cultured human osteoclast cells directly on human cadaveric bone slices from different age groups (young and elderly) to warrant its relevance to in vivo conditions. The cell culture was terminated on the 3rd, 7th, and 10th day, respectively, to assess temporal changes in the number of differentiated osteoclasts, the number and size of osteoclastic resorption pits, the amount of bone resorbed, as well as the amount of matrix AGEs released in the medium by resorption. In addition, the in situ concentration of matrix AGEs at each resorption pit was also estimated based on its AGEs autofluorescent intensity. The results indicated that (1) osteoclastic resorption activities were significantly correlated with the donor age, showing larger but shallower resorption pits on the elderly bone substrates than on the younger ones; (2) osteoclast resorption activities were not significantly dependent on the in situ AGEs concentration in bone matrix, and (3) a correlation was observed between osteoclast activities and the concentration of AGEs released by the resorption. These results suggest that osteoclasts tend to migrate away from initial anchoring sites on elderly bone substrate during resorption compared to younger bone substrates. However, such behavior is not directly related to the in situ concentration of AGEs in bone matrix at the resorption sites. Springer Science+Business Media New York, 2015 Bone nationallicence Osteoclast nationallicence Advanced glycation end products (AGEs) nationallicence Bone resorption nationallicence Yang Xiao School of Biological Sciences and Medical Engineering, Beihang University, Beijing, China aut Gandhi Chintan Departments of Biomedical Engineering, The University of Texas at San Antonio (UTSA), One UTSA Circle, 78249, San Antonio, TX, USA aut Rahman MD Department of Medicine, University of Texas Health Science Center at San Antonio (UTHSCSA), San Antonio, USA aut Appleford Mark Departments of Biomedical Engineering, The University of Texas at San Antonio (UTSA), One UTSA Circle, 78249, San Antonio, TX, USA aut Sun Lian-Wen School of Biological Sciences and Medical Engineering, Beihang University, Beijing, China aut Wang Xiaodu Departments of Biomedical Engineering, The University of Texas at San Antonio (UTSA), One UTSA Circle, 78249, San Antonio, TX, USA aut Calcified Tissue International Springer US; http://www.springer-ny.com 97/6(2015-12-01), 592-601 0171-967X 97:6<592 2015 97 223 https://doi.org/10.1007/s00223-015-0042-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00223-015-0042-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Xiao School of Biological Sciences and Medical Engineering, Beihang University, Beijing, China aut NATIONALLICENCE 700 1- Gandhi Chintan Departments of Biomedical Engineering, The University of Texas at San Antonio (UTSA), One UTSA Circle, 78249, San Antonio, TX, USA aut NATIONALLICENCE 700 1- Rahman MD Department of Medicine, University of Texas Health Science Center at San Antonio (UTHSCSA), San Antonio, USA aut NATIONALLICENCE 700 1- Appleford Mark Departments of Biomedical Engineering, The University of Texas at San Antonio (UTSA), One UTSA Circle, 78249, San Antonio, TX, USA aut NATIONALLICENCE 700 1- Sun Lian-Wen School of Biological Sciences and Medical Engineering, Beihang University, Beijing, China aut NATIONALLICENCE 700 1- Wang Xiaodu Departments of Biomedical Engineering, The University of Texas at San Antonio (UTSA), One UTSA Circle, 78249, San Antonio, TX, USA aut NATIONALLICENCE 773 0- Calcified Tissue International Springer US; http://www.springer-ny.com 97/6(2015-12-01), 592-601 0171-967X 97:6<592 2015 97 223