caa a22 4500 605520763 CHVBK 20210128100737.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00223-015-0019-0 doi (NATIONALLICENCE)springer-10.1007/s00223-015-0019-0 The Impact of Strontium Ranelate on Metaphyseal Bone Healing in Ovariectomized Rats [Elektronische Daten] [Marina Komrakova, Anna Weidemann, Christian Dullin, Joachim Ebert, Mohammad Tezval, Klaus Stuermer, Stephan Sehmisch] The following questions were addressed: whether therapy with strontium ranelate (SR) should be continued or interrupted if the fractures occur during SR treatment and whether SR could be applied directly after fracture to improve bone healing. Sprague-Dawley rats (3month old) were ovariectomized (Ovx, n=48) or left intact (n=12). After 8weeks, a bilateral transverse osteotomy of the tibia metaphysis was created in all rats. Ovx rats were divided into four groups: Ovx; SR applied directly after Ovx until osteotomy (prophylaxis, SR pr, 8weeks); SR applied after osteotomy (therapy, SR th, 5weeks); SR applied during the whole experiment (pr+th, 13weeks). SR dosage was 625mg/kg body weight/day, administered in the feed. Five weeks later, tibiae were analyzed by biomechanical, histological, micro-CT, and gene expression analyses. The SR pr+th treatment increased total bone mineral density (BMD), bone volume fraction, cortical BMD and volume, callus area and density, serum alkaline phosphatase, tartrate-resistant acid phosphatase mRNA, accelerated osteotomy bridging, and callus formation at weeks 2 and 3 of healing and decreased the osteoprotegerin/receptor activator of nuclear factor kB ligand mRNA ratio. SR th enlarged callus area and improved callus formation during the 5th week of healing. SR pr improved cortical BMD preserving bone after SR discontinuation (5-week rest); the bone healing was not affected. SR content in the tibia metaphysis was the highest in SR pr+th group and was not different between SR pr and SR th. SR has a positive effect on osteoporotic bone healing in rat and SR treatment can be continued after the fracture occurs or applied directly after the fracture. Springer Science+Business Media New York, 2015 Strontium ranelate nationallicence Bone healing nationallicence Osteoporosis nationallicence Rat model nationallicence Komrakova Marina Department of Trauma Surgery and Reconstructive Surgery, University Medical Center Göttingen, Robert-Koch St. 40, 37075, Göttingen, Germany aut Weidemann Anna Department of Trauma Surgery and Reconstructive Surgery, University Medical Center Göttingen, Robert-Koch St. 40, 37075, Göttingen, Germany aut Dullin Christian Department of Radiology, University of Göttingen, Robert-Koch 40, 37075, Göttingen, Germany aut Ebert Joachim Department of Medical Microbiology, Subdivision of General Hygiene and Environmental Health, University of Göttingen, Humboldallee 34a, 37073, Göttingen, Germany aut Tezval Mohammad Department of Trauma Surgery and Reconstructive Surgery, University Medical Center Göttingen, Robert-Koch St. 40, 37075, Göttingen, Germany aut Stuermer Klaus Department of Trauma Surgery and Reconstructive Surgery, University Medical Center Göttingen, Robert-Koch St. 40, 37075, Göttingen, Germany aut Sehmisch Stephan Department of Trauma Surgery and Reconstructive Surgery, University Medical Center Göttingen, Robert-Koch St. 40, 37075, Göttingen, Germany aut Calcified Tissue International Springer US; http://www.springer-ny.com 97/4(2015-10-01), 391-401 0171-967X 97:4<391 2015 97 223 https://doi.org/10.1007/s00223-015-0019-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00223-015-0019-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Komrakova Marina Department of Trauma Surgery and Reconstructive Surgery, University Medical Center Göttingen, Robert-Koch St. 40, 37075, Göttingen, Germany aut NATIONALLICENCE 700 1- Weidemann Anna Department of Trauma Surgery and Reconstructive Surgery, University Medical Center Göttingen, Robert-Koch St. 40, 37075, Göttingen, Germany aut NATIONALLICENCE 700 1- Dullin Christian Department of Radiology, University of Göttingen, Robert-Koch 40, 37075, Göttingen, Germany aut NATIONALLICENCE 700 1- Ebert Joachim Department of Medical Microbiology, Subdivision of General Hygiene and Environmental Health, University of Göttingen, Humboldallee 34a, 37073, Göttingen, Germany aut NATIONALLICENCE 700 1- Tezval Mohammad Department of Trauma Surgery and Reconstructive Surgery, University Medical Center Göttingen, Robert-Koch St. 40, 37075, Göttingen, Germany aut NATIONALLICENCE 700 1- Stuermer Klaus Department of Trauma Surgery and Reconstructive Surgery, University Medical Center Göttingen, Robert-Koch St. 40, 37075, Göttingen, Germany aut NATIONALLICENCE 700 1- Sehmisch Stephan Department of Trauma Surgery and Reconstructive Surgery, University Medical Center Göttingen, Robert-Koch St. 40, 37075, Göttingen, Germany aut NATIONALLICENCE 773 0- Calcified Tissue International Springer US; http://www.springer-ny.com 97/4(2015-10-01), 391-401 0171-967X 97:4<391 2015 97 223