caa a22 4500 605520860 CHVBK 20210128100737.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00223-015-0036-z doi (NATIONALLICENCE)springer-10.1007/s00223-015-0036-z Serum Circulating MicroRNAs as Biomarkers of Osteoporotic Fracture [Elektronische Daten] [Layla Panach, Damián Mifsut, Juan Tarín, Antonio Cano, Miguel García-Pérez] Osteoporosis is a common skeletal disorder characterized by increased risk of bone fracture (BF) due to fragility. BFs, particularly hip fracture, are a major concern in health care because of the associated morbidity and mortality, mainly in the elderly. Lately the involvement of epigenetic mechanisms in the pathophysiology of many diseases has been recognized. In this context, the identification of microRNAs (miRNAs) specific to BF should represent a substantial step forward in diagnostics and therapeutics. The present study aimed to identify specific miRNAs in osteoporotic BF patients compared to those in osteoarthritic controls. In the profiling stage, total RNA was extracted from serum, two pools were prepared, and then retro-transcribed in triplicate. Levels of 179 serum miRNAs were analyzed by real-time PCR, and 42 of them showed significance (P<0.05), and 12 passed the false discovery rate test for multiple comparisons. Six miRNAs were selected for the replication stage and individually analyzed in sera from 15 BF patients and 12 controls. Results showed that 3 miRNAs (miR-122-5p, miR-125b-5p, and miR-21-5p) were valuable upregulated biomarkers in BF with respect to controls and, significantly, their levels were not affected by hemolysis. For miR-21-5p, the difference detected between groups was independent of age (P=0.005) and its levels correlated to those of CTx (r=0.76; P<0.00001), a marker of bone resorption. In conclusion, several miRNAs may be biomarkers of BF, particularly miR-21-5p. Further studies are needed in order to better characterize the levels of these miRNAs in other bone diseases and to elucidate the mechanism involved in the association of these three miRNAs with osteoporotic BF. Springer Science+Business Media New York, 2015 miRNA nationallicence Bone fracture nationallicence Osteoporosis nationallicence Biomarker nationallicence Panach Layla Research Foundation, Institute of Health Research INCLIVA, Av. Menéndez Pelayo, 4 Accesorio, 46010, Valencia, Spain aut Mifsut Damián Orthopedic Surgery and Traumatology, Clinic Hospital, Institute of Health Research INCLIVA, 46010, Valencia, Spain aut Tarín Juan Department of Functional Biology and Physical Anthropology, University of Valencia, 46100, Burjassot, Spain aut Cano Antonio Department of Pediatrics, Obstetrics and Gynecology, University of Valencia, 46010, Valencia, Spain aut García-Pérez Miguel Research Foundation, Institute of Health Research INCLIVA, Av. Menéndez Pelayo, 4 Accesorio, 46010, Valencia, Spain aut Calcified Tissue International Springer US; http://www.springer-ny.com 97/5(2015-11-01), 495-505 0171-967X 97:5<495 2015 97 223 https://doi.org/10.1007/s00223-015-0036-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00223-015-0036-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Panach Layla Research Foundation, Institute of Health Research INCLIVA, Av. Menéndez Pelayo, 4 Accesorio, 46010, Valencia, Spain aut NATIONALLICENCE 700 1- Mifsut Damián Orthopedic Surgery and Traumatology, Clinic Hospital, Institute of Health Research INCLIVA, 46010, Valencia, Spain aut NATIONALLICENCE 700 1- Tarín Juan Department of Functional Biology and Physical Anthropology, University of Valencia, 46100, Burjassot, Spain aut NATIONALLICENCE 700 1- Cano Antonio Department of Pediatrics, Obstetrics and Gynecology, University of Valencia, 46010, Valencia, Spain aut NATIONALLICENCE 700 1- García-Pérez Miguel Research Foundation, Institute of Health Research INCLIVA, Av. Menéndez Pelayo, 4 Accesorio, 46010, Valencia, Spain aut NATIONALLICENCE 773 0- Calcified Tissue International Springer US; http://www.springer-ny.com 97/5(2015-11-01), 495-505 0171-967X 97:5<495 2015 97 223