caa a22 4500 60552095X CHVBK 20210128100737.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00223-015-9998-0 doi (NATIONALLICENCE)springer-10.1007/s00223-015-9998-0 Three-Times-Weekly Administration of Teriparatide Improves Vertebral and Peripheral Bone Density, Microarchitecture, and Mechanical Properties Without Accelerating Bone Resorption in Ovariectomized Rats [Elektronische Daten] [Ryoko Takao-Kawabata, Yukihiro Isogai, Aya Takakura, Yukari Shimazu, Emika Sugimoto, Osamu Nakazono, Ichiro Ikegaki, Hiroshi Kuriyama, Shinya Tanaka, Hiromi Oda, Toshinori Ishizuya] Daily and weekly administration of teriparatide (PTH1-34) reduces the risk of osteoporotic bone fractures. However, their effects on markers of bone formation and bone resorption differ. These results indicate that the dosing frequency of teriparatide may affect bone metabolism and bone structure, with different effects on bone strength. In the present study, to evaluate the dose-related effects of a low administration frequency of teriparatide on bone status, we investigated the effects of three-times-weekly administration of teriparatide (1.1, 5.6, or 28.2µg/kg) for 12months on bone parameters, including bone metabolism markers, bone mineral density (BMD), micro-computed tomography, and bone strength, using 6-month-old ovariectomized (OVX) rats. Three-times-weekly administration of teriparatide dose-dependently increased the BMD of the lumbar vertebra and femur in OVX rats, and increased serum osteocalcin (a marker of bone formation), but not type I collagen C-telopeptide (a marker of bone resorption). The trabecular number and thickness increased in the vertebrae and femur, as in prior reports of daily teriparatide administration in OVX rats. Cortical thickness increased only toward the endocortical side of the femur, unlike with daily administration. Bone strength of the vertebrae and proximal and shaft of the femur was correlated with the changes in BMD and bone structure. These results demonstrate the effects of low frequency, intermittent administration of teriparatide on the biomechanical, and microstructural properties of bone in OVX rats. The Author(s), 2015 Teriparatide nationallicence Three-times-weekly nationallicence Bone metabolism nationallicence Mechanical strength nationallicence Micro-computed tomography nationallicence Takao-Kawabata Ryoko Laboratory for Pharmacology, Pharmaceuticals Research Center, Asahi Kasei Pharma Corporation, 632-1 Mifuku, Izunokuni, 410-2321, Shizuoka, Japan aut Isogai Yukihiro Laboratory for Pharmacology, Pharmaceuticals Research Center, Asahi Kasei Pharma Corporation, 632-1 Mifuku, Izunokuni, 410-2321, Shizuoka, Japan aut Takakura Aya Laboratory for Pharmacology, Pharmaceuticals Research Center, Asahi Kasei Pharma Corporation, 632-1 Mifuku, Izunokuni, 410-2321, Shizuoka, Japan aut Shimazu Yukari Laboratory for Pharmacology, Pharmaceuticals Research Center, Asahi Kasei Pharma Corporation, 632-1 Mifuku, Izunokuni, 410-2321, Shizuoka, Japan aut Sugimoto Emika Laboratory for Pharmacology, Pharmaceuticals Research Center, Asahi Kasei Pharma Corporation, 632-1 Mifuku, Izunokuni, 410-2321, Shizuoka, Japan aut Nakazono Osamu Laboratory for Pharmacology, Pharmaceuticals Research Center, Asahi Kasei Pharma Corporation, 632-1 Mifuku, Izunokuni, 410-2321, Shizuoka, Japan aut Ikegaki Ichiro Laboratory for Pharmacology, Pharmaceuticals Research Center, Asahi Kasei Pharma Corporation, 632-1 Mifuku, Izunokuni, 410-2321, Shizuoka, Japan aut Kuriyama Hiroshi Laboratory for Pharmacology, Pharmaceuticals Research Center, Asahi Kasei Pharma Corporation, 632-1 Mifuku, Izunokuni, 410-2321, Shizuoka, Japan aut Tanaka Shinya Department of Orthopaedic Surgery, Saitama Medical University, 38 Morohongo, Moroyama-cho, Iruma-gun, 350-0495, Saitama, Japan aut Oda Hiromi Department of Orthopaedic Surgery, Saitama Medical University, 38 Morohongo, Moroyama-cho, Iruma-gun, 350-0495, Saitama, Japan aut Ishizuya Toshinori Laboratory for Pharmacology, Pharmaceuticals Research Center, Asahi Kasei Pharma Corporation, 632-1 Mifuku, Izunokuni, 410-2321, Shizuoka, Japan aut Calcified Tissue International Springer US; http://www.springer-ny.com 97/2(2015-08-01), 156-168 0171-967X 97:2<156 2015 97 223 https://doi.org/10.1007/s00223-015-9998-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00223-015-9998-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takao-Kawabata Ryoko Laboratory for Pharmacology, Pharmaceuticals Research Center, Asahi Kasei Pharma Corporation, 632-1 Mifuku, Izunokuni, 410-2321, Shizuoka, Japan aut NATIONALLICENCE 700 1- Isogai Yukihiro Laboratory for Pharmacology, Pharmaceuticals Research Center, Asahi Kasei Pharma Corporation, 632-1 Mifuku, Izunokuni, 410-2321, Shizuoka, Japan aut NATIONALLICENCE 700 1- Takakura Aya Laboratory for Pharmacology, Pharmaceuticals Research Center, Asahi Kasei Pharma Corporation, 632-1 Mifuku, Izunokuni, 410-2321, Shizuoka, Japan aut NATIONALLICENCE 700 1- Shimazu Yukari Laboratory for Pharmacology, Pharmaceuticals Research Center, Asahi Kasei Pharma Corporation, 632-1 Mifuku, Izunokuni, 410-2321, Shizuoka, Japan aut NATIONALLICENCE 700 1- Sugimoto Emika Laboratory for Pharmacology, Pharmaceuticals Research Center, Asahi Kasei Pharma Corporation, 632-1 Mifuku, Izunokuni, 410-2321, Shizuoka, Japan aut NATIONALLICENCE 700 1- Nakazono Osamu Laboratory for Pharmacology, Pharmaceuticals Research Center, Asahi Kasei Pharma Corporation, 632-1 Mifuku, Izunokuni, 410-2321, Shizuoka, Japan aut NATIONALLICENCE 700 1- Ikegaki Ichiro Laboratory for Pharmacology, Pharmaceuticals Research Center, Asahi Kasei Pharma Corporation, 632-1 Mifuku, Izunokuni, 410-2321, Shizuoka, Japan aut NATIONALLICENCE 700 1- Kuriyama Hiroshi Laboratory for Pharmacology, Pharmaceuticals Research Center, Asahi Kasei Pharma Corporation, 632-1 Mifuku, Izunokuni, 410-2321, Shizuoka, Japan aut NATIONALLICENCE 700 1- Tanaka Shinya Department of Orthopaedic Surgery, Saitama Medical University, 38 Morohongo, Moroyama-cho, Iruma-gun, 350-0495, Saitama, Japan aut NATIONALLICENCE 700 1- Oda Hiromi Department of Orthopaedic Surgery, Saitama Medical University, 38 Morohongo, Moroyama-cho, Iruma-gun, 350-0495, Saitama, Japan aut NATIONALLICENCE 700 1- Ishizuya Toshinori Laboratory for Pharmacology, Pharmaceuticals Research Center, Asahi Kasei Pharma Corporation, 632-1 Mifuku, Izunokuni, 410-2321, Shizuoka, Japan aut NATIONALLICENCE 773 0- Calcified Tissue International Springer US; http://www.springer-ny.com 97/2(2015-08-01), 156-168 0171-967X 97:2<156 2015 97 223