caa a22 4500 605520984 CHVBK 20210128100738.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00223-015-0023-4 doi (NATIONALLICENCE)springer-10.1007/s00223-015-0023-4 Characterisation of Osteoprotegerin Autoantibodies in Coeliac Disease [Elektronische Daten] [Ana Real, Nick Gilbert, Barbara Hauser, Nick Kennedy, Alan Shand, Helen Gillett, Peter Gillett, Clive Goddard, Ángel Cebolla, Carolina Sousa, William Fraser, Jack Satsangi, Stuart Ralston, Philip Riches] Autoantibodies neutralising the effect of the bone regulatory cytokine osteoprotegerin (OPG) have been described in a patient with severe osteoporosis and coeliac disease. This study aimed to determine the prevalence and epitope specificity of autoantibodies to OPG in patients with coeliac disease, and correlate their presence with bone mineral density. A direct enzyme-linked immunosorbent assay was developed and used to screen patients with coeliac disease for autoantibodies to OPG. Recombinant fragments of OPG were made to evaluate the epitope specificity and affinity of these antibodies. Phenotype information of the patients was obtained by case note review. Raised titres of antibodies to OPG were found in 7/71 (9.8%) patients with coeliac disease, compared with 1/72 (1.4%) non-coeliac osteoporosis clinic control patients (p<0.05). Our results suggest that a polyclonal antibody response to OPG is raised in these patients capable of recognising different epitopes of OPG with varying affinity. The titre of OPG antibodies was associated with lower bone mineral density Z-score of the hip in coeliac patients on univariate (p<0.05) and multivariate analysis including age, sex height and weight as covariates (p<0.01). Polyclonal antibodies to OPG are more common in patients with coeliac disease and are independently associated with lower bone mineral density Z-scores of the hip. Further work is required to establish the clinical utility of testing for OPG antibodies. Springer Science+Business Media New York, 2015 Osteoporosis nationallicence Coeliac disease nationallicence Osteoprotegerin nationallicence Autoantibodies nationallicence Real Ana Centre for Genomics and Experimental Medicine, Institute of Genetics and Molecular Medicine (IGMM), Edinburgh, EH4 2XU, UK aut Gilbert Nick Human Genetics Unit, IGMM, Edinburgh, UK aut Hauser Barbara Centre for Genomics and Experimental Medicine, Institute of Genetics and Molecular Medicine (IGMM), Edinburgh, EH4 2XU, UK aut Kennedy Nick Centre for Genomics and Experimental Medicine, Institute of Genetics and Molecular Medicine (IGMM), Edinburgh, EH4 2XU, UK aut Shand Alan Gastroenterology Department, Lothian University Hospitals NHS Trust, Edinburgh, UK aut Gillett Helen Gastroenterology Department, Lothian University Hospitals NHS Trust, Edinburgh, UK aut Gillett Peter Gastroenterology Department, Lothian University Hospitals NHS Trust, Edinburgh, UK aut Goddard Clive Gastroenterology Department, Lothian University Hospitals NHS Trust, Edinburgh, UK aut Cebolla Ángel Biomedal SL, Seville, Spain aut Sousa Carolina Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, Seville, Spain aut Fraser William University of East Anglia, Norwich, UK aut Satsangi Jack Centre for Genomics and Experimental Medicine, Institute of Genetics and Molecular Medicine (IGMM), Edinburgh, EH4 2XU, UK aut Ralston Stuart Centre for Genomics and Experimental Medicine, Institute of Genetics and Molecular Medicine (IGMM), Edinburgh, EH4 2XU, UK aut Riches Philip Centre for Genomics and Experimental Medicine, Institute of Genetics and Molecular Medicine (IGMM), Edinburgh, EH4 2XU, UK aut Calcified Tissue International Springer US; http://www.springer-ny.com 97/2(2015-08-01), 125-133 0171-967X 97:2<125 2015 97 223 https://doi.org/10.1007/s00223-015-0023-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00223-015-0023-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Real Ana Centre for Genomics and Experimental Medicine, Institute of Genetics and Molecular Medicine (IGMM), Edinburgh, EH4 2XU, UK aut NATIONALLICENCE 700 1- Gilbert Nick Human Genetics Unit, IGMM, Edinburgh, UK aut NATIONALLICENCE 700 1- Hauser Barbara Centre for Genomics and Experimental Medicine, Institute of Genetics and Molecular Medicine (IGMM), Edinburgh, EH4 2XU, UK aut NATIONALLICENCE 700 1- Kennedy Nick Centre for Genomics and Experimental Medicine, Institute of Genetics and Molecular Medicine (IGMM), Edinburgh, EH4 2XU, UK aut NATIONALLICENCE 700 1- Shand Alan Gastroenterology Department, Lothian University Hospitals NHS Trust, Edinburgh, UK aut NATIONALLICENCE 700 1- Gillett Helen Gastroenterology Department, Lothian University Hospitals NHS Trust, Edinburgh, UK aut NATIONALLICENCE 700 1- Gillett Peter Gastroenterology Department, Lothian University Hospitals NHS Trust, Edinburgh, UK aut NATIONALLICENCE 700 1- Goddard Clive Gastroenterology Department, Lothian University Hospitals NHS Trust, Edinburgh, UK aut NATIONALLICENCE 700 1- Cebolla Ángel Biomedal SL, Seville, Spain aut NATIONALLICENCE 700 1- Sousa Carolina Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, Seville, Spain aut NATIONALLICENCE 700 1- Fraser William University of East Anglia, Norwich, UK aut NATIONALLICENCE 700 1- Satsangi Jack Centre for Genomics and Experimental Medicine, Institute of Genetics and Molecular Medicine (IGMM), Edinburgh, EH4 2XU, UK aut NATIONALLICENCE 700 1- Ralston Stuart Centre for Genomics and Experimental Medicine, Institute of Genetics and Molecular Medicine (IGMM), Edinburgh, EH4 2XU, UK aut NATIONALLICENCE 700 1- Riches Philip Centre for Genomics and Experimental Medicine, Institute of Genetics and Molecular Medicine (IGMM), Edinburgh, EH4 2XU, UK aut NATIONALLICENCE 773 0- Calcified Tissue International Springer US; http://www.springer-ny.com 97/2(2015-08-01), 125-133 0171-967X 97:2<125 2015 97 223