caa a22 4500 605521085 CHVBK 20210128100738.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00223-014-9928-6 doi (NATIONALLICENCE)springer-10.1007/s00223-014-9928-6 Primary Cilia Exist in a Small Fraction of Cells in Trabecular Bone and Marrow [Elektronische Daten] [Thomas Coughlin, Muriel Voisin, Mitchell Schaffler, Glen Niebur, Laoise McNamara] Primary cilia are potent mechanical and chemical sensory organelles in cells of bone lineage in tissue culture. Cell culture experiments suggest that primary cilia sense fluid flow and this stimulus is translated through biochemical signaling into an osteogenic response in bone cells. Moreover, in vivo, primary cilia knockout in bone cells attenuates bone formation in response to loading. However, understanding the role of the primary cilium in bone mechanotransduction requires knowledge of its incidence and location in vivo. We used immunohistochemistry to quantify the number of cells with primary cilia within the trabecular bone tissue and the enclosed marrow of ovine cervical vertebrae. Primary cilia were identified in osteocytes, bone lining cells, and in cells within the marrow, but were present in only a small fraction of cells. Approximately 4% of osteocytes and 4.6% of bone lining cells expressed primary cilia. Within the marrow space, only approximately 1% of cells presented primary cilia. The low incidence of primary cilia may indicate that cilia either function as mechanosensors in a selected number of cells, function in concert with other mechanosensing mechanisms, or that the role of primary cilia in mechanosensing is secondary to its role in chemosensing or cellular attachment. Springer Science+Business Media New York, 2014 Mechanobiology nationallicence Mechanotransduction nationallicence Primary cilia nationallicence Marrow nationallicence Osteocyte nationallicence MSCs nationallicence Coughlin Thomas Tissue Mechanics Laboratory, Bioengineering Graduate Program, University of Notre Dame, Notre Dame, IN, USA aut Voisin Muriel Department of Mechanical and Biomedical Engineering, National University of Ireland, Galway, Galway, Ireland aut Schaffler Mitchell Department of Biomedical Engineering, Grove School of Engineering, City College of New York, New York, USA aut Niebur Glen Tissue Mechanics Laboratory, Bioengineering Graduate Program, University of Notre Dame, Notre Dame, IN, USA aut McNamara Laoise Department of Mechanical and Biomedical Engineering, National University of Ireland, Galway, Galway, Ireland aut Calcified Tissue International Springer US; http://www.springer-ny.com 96/1(2015-01-01), 65-72 0171-967X 96:1<65 2015 96 223 https://doi.org/10.1007/s00223-014-9928-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00223-014-9928-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Coughlin Thomas Tissue Mechanics Laboratory, Bioengineering Graduate Program, University of Notre Dame, Notre Dame, IN, USA aut NATIONALLICENCE 700 1- Voisin Muriel Department of Mechanical and Biomedical Engineering, National University of Ireland, Galway, Galway, Ireland aut NATIONALLICENCE 700 1- Schaffler Mitchell Department of Biomedical Engineering, Grove School of Engineering, City College of New York, New York, USA aut NATIONALLICENCE 700 1- Niebur Glen Tissue Mechanics Laboratory, Bioengineering Graduate Program, University of Notre Dame, Notre Dame, IN, USA aut NATIONALLICENCE 700 1- McNamara Laoise Department of Mechanical and Biomedical Engineering, National University of Ireland, Galway, Galway, Ireland aut NATIONALLICENCE 773 0- Calcified Tissue International Springer US; http://www.springer-ny.com 96/1(2015-01-01), 65-72 0171-967X 96:1<65 2015 96 223