caa a22 4500 60552114X CHVBK 20210128100738.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00223-015-9981-9 doi (NATIONALLICENCE)springer-10.1007/s00223-015-9981-9 Three-Dimensional Porous Gelapin-Simvastatin Scaffolds Promoted Bone Defect Healing in Rabbits [Elektronische Daten] [Ali Moshiri, Mostafa Shahrezaee, Babak Shekarchi, Ahmad Oryan, Kamran Azma] Treatment of large bone defects (LBDs) is technically demanding. Tissue engineering is an option. A bioactive graft may be produced by combining tissue scaffolds and healing promotive factors in order to accelerate bone repair. We investigated the role of Simvastatin (Sim)-embedded porous Gelapin (Gel) scaffold on experimental bone healing. At first, the effectiveness of different concentrations of Gel and Sim powders was investigated in an experimentally induced femoral hole model in rabbits (n=6) for 30days. Then bone bioactive grafts were produced by combination of the effective concentrations of Gel, Sim, and Genipin. The bioimplants were subcutaneously tested in a rabbit model (n=9) to determine their biocompatibility and biodegradability for 10-30days. Finally, a large radial bone defect model was produced in rabbits (n=20), and the bioimplants were inserted in the defects. The untreated and autograft-treated bone defects were served as controls. The animals were euthanized after 30 and 60days of bone injury. The bone samples were evaluated by radiography, three-dimensional CT scan, bone densitometry, histopathology, and nano-indentation. At a concentration of 5mg/hole, Sim closed the femoral bone holes after 30days, while in the defect, autograft, and Gel groups, the holes were open. Both the Gel and Gel-Sim scaffolds were biocompatible and biodegradable. Subcutaneously, the Gel-Sim scaffold was replaced with the newly regenerated ectopic bone after 30days. After implantation of the Gel-Sim scaffold in the radial bone defects, the scaffold was completely replaced with new woven bone after 30days which was then matured and remodeled into a cortical bone after 60days. Sixty days after bone injury, the Gel-Sim-treated defects had significantly higher bone volume, matrix mineralization, elastic modulus, and contact hardness when compared to the controls. The Gel-Sim scaffold may be a suitable option in managing LBDs. Springer Science+Business Media New York, 2015 Gelapin nationallicence Simvastatin nationallicence Bone nationallicence Healing nationallicence Tissue engineering nationallicence Regenerative medicine nationallicence Moshiri Ali Department of Orthopedic Surgery, School of Medicine, AJA University of Medical Science, Tehran, Iran aut Shahrezaee Mostafa Department of Orthopedic Surgery, School of Medicine, AJA University of Medical Science, Tehran, Iran aut Shekarchi Babak Department of Radiology, School of Medicine, AJA University of Medical Science, Tehran, Iran aut Oryan Ahmad Department of Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran aut Azma Kamran Physical Medicine and Rehabilitation Department, School of Medicine, AJA University of Medical Science, Tehran, Iran aut Calcified Tissue International Springer US; http://www.springer-ny.com 96/6(2015-06-01), 552-564 0171-967X 96:6<552 2015 96 223 https://doi.org/10.1007/s00223-015-9981-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00223-015-9981-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moshiri Ali Department of Orthopedic Surgery, School of Medicine, AJA University of Medical Science, Tehran, Iran aut NATIONALLICENCE 700 1- Shahrezaee Mostafa Department of Orthopedic Surgery, School of Medicine, AJA University of Medical Science, Tehran, Iran aut NATIONALLICENCE 700 1- Shekarchi Babak Department of Radiology, School of Medicine, AJA University of Medical Science, Tehran, Iran aut NATIONALLICENCE 700 1- Oryan Ahmad Department of Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran aut NATIONALLICENCE 700 1- Azma Kamran Physical Medicine and Rehabilitation Department, School of Medicine, AJA University of Medical Science, Tehran, Iran aut NATIONALLICENCE 773 0- Calcified Tissue International Springer US; http://www.springer-ny.com 96/6(2015-06-01), 552-564 0171-967X 96:6<552 2015 96 223