caa a22 4500 605521190 CHVBK 20210128100739.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00223-015-9982-8 doi (NATIONALLICENCE)springer-10.1007/s00223-015-9982-8 Caffeic Acid Phenethyl Ester Abrogates Bone Resorption in a Murine Calvarial Model of Polyethylene Particle-Induced Osteolysis [Elektronische Daten] [M. Zawawi, E. Perilli, R. Stansborough, V. Marino, M. Cantley, J. Xu, A. Dharmapatni, D. Haynes, R. Gibson, T. Crotti] Particle-induced bone loss by osteoclasts is a common cause of aseptic loosening around implants. This study investigates whether caffeic acid phenethyl ester (CAPE), a potent and specific inhibitor of nuclear factor of activated T cells, cytoplasmic, calcineurin-dependent 1 and nuclear factor kappa B, at a low dose reduces bone resorption in a murine calvarial model of polyethylene (PE) particle-induced osteolysis. The effects of particles and CAPE treatment on gastrointestinal tract (GIT) histopathology were also evaluated. Mice were scanned using in vivo animal micro-computed tomography (μCT) as a baseline measurement. PE particles (2.82×109particles/mL) were implanted over the calvariae on day 0. CAPE was administered subcutaneously (1mg/kg/day) at days 0, 4, 7 and 10. Mice were killed at day 14 and serum was analysed for Type-1 carboxyterminal collagen crosslinks (CTX)-1 and osteoclast-associated receptor (OSCAR) levels. Ex vivo μCT scans were conducted to assess bone volume (BV) change and percentage area of calvarial surface resorbed. Calvarial and GIT tissue was processed for histopathology. By day 14, PE particles significantly induced calvarial bone loss compared with control animals as evidenced by resorption areas adjacent to the implanted PE in three-dimensional μCT images, an increase in percentage of resorbed area (p=0.0022), reduction in BV (p=0.0012) and increased Tartrate-resistant acid phosphatase positive cells. Serum CTX-1 (p=0.0495) and OSCAR levels (p=0.0006) significantly increased in the PE implant group. CAPE significantly inhibited PE particle-induced calvarial osteolysis, as evidenced by a significant reduction in surface bone resorption (p=0.0012) and volumetric change (p=0.0154) compared with PE only, but had no effect on systemic CTX-1. Neither particles nor CAPE had an effect on GIT histopathology. Springer Science+Business Media New York, 2015 Osteolysis nationallicence CAPE nationallicence NF kappa B inhibitor nationallicence Micro-CT nationallicence CTX-1 nationallicence OSCAR nationallicence Zawawi M. Discipline of Anatomy and Pathology, School of Medical Sciences, The University of Adelaide, 5000, Adelaide, SA, Australia aut Perilli E. Medical Device Research Institute, School of Computer Science, Engineering and Mathematics, Flinders University, Clovelly Park, SA, Australia aut Stansborough R. Discipline of Anatomy and Pathology, School of Medical Sciences, The University of Adelaide, 5000, Adelaide, SA, Australia aut Marino V. School of Dentistry, The University of Adelaide, Adelaide, SA, Australia aut Cantley M. Discipline of Anatomy and Pathology, School of Medical Sciences, The University of Adelaide, 5000, Adelaide, SA, Australia aut Xu J. School of Pathology and Laboratory Medicine, The University of Western Australia, Crawley, WA, Australia aut Dharmapatni A. Discipline of Anatomy and Pathology, School of Medical Sciences, The University of Adelaide, 5000, Adelaide, SA, Australia aut Haynes D. Discipline of Anatomy and Pathology, School of Medical Sciences, The University of Adelaide, 5000, Adelaide, SA, Australia aut Gibson R. Discipline of Anatomy and Pathology, School of Medical Sciences, The University of Adelaide, 5000, Adelaide, SA, Australia aut Crotti T. Discipline of Anatomy and Pathology, School of Medical Sciences, The University of Adelaide, 5000, Adelaide, SA, Australia aut Calcified Tissue International Springer US; http://www.springer-ny.com 96/6(2015-06-01), 565-574 0171-967X 96:6<565 2015 96 223 https://doi.org/10.1007/s00223-015-9982-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00223-015-9982-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zawawi M. Discipline of Anatomy and Pathology, School of Medical Sciences, The University of Adelaide, 5000, Adelaide, SA, Australia aut NATIONALLICENCE 700 1- Perilli E. Medical Device Research Institute, School of Computer Science, Engineering and Mathematics, Flinders University, Clovelly Park, SA, Australia aut NATIONALLICENCE 700 1- Stansborough R. Discipline of Anatomy and Pathology, School of Medical Sciences, The University of Adelaide, 5000, Adelaide, SA, Australia aut NATIONALLICENCE 700 1- Marino V. School of Dentistry, The University of Adelaide, Adelaide, SA, Australia aut NATIONALLICENCE 700 1- Cantley M. Discipline of Anatomy and Pathology, School of Medical Sciences, The University of Adelaide, 5000, Adelaide, SA, Australia aut NATIONALLICENCE 700 1- Xu J. School of Pathology and Laboratory Medicine, The University of Western Australia, Crawley, WA, Australia aut NATIONALLICENCE 700 1- Dharmapatni A. Discipline of Anatomy and Pathology, School of Medical Sciences, The University of Adelaide, 5000, Adelaide, SA, Australia aut NATIONALLICENCE 700 1- Haynes D. Discipline of Anatomy and Pathology, School of Medical Sciences, The University of Adelaide, 5000, Adelaide, SA, Australia aut NATIONALLICENCE 700 1- Gibson R. Discipline of Anatomy and Pathology, School of Medical Sciences, The University of Adelaide, 5000, Adelaide, SA, Australia aut NATIONALLICENCE 700 1- Crotti T. Discipline of Anatomy and Pathology, School of Medical Sciences, The University of Adelaide, 5000, Adelaide, SA, Australia aut NATIONALLICENCE 773 0- Calcified Tissue International Springer US; http://www.springer-ny.com 96/6(2015-06-01), 565-574 0171-967X 96:6<565 2015 96 223