caa a22 4500 605521255 CHVBK 20210128100739.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00223-014-9932-x doi (NATIONALLICENCE)springer-10.1007/s00223-014-9932-x Effects of 1,25-Dihydroxyvitamin D3 and Vitamin D3 on the Expression of the Vitamin D Receptor in Human Skeletal Muscle Cells [Elektronische Daten] [Rachele Pojednic, Lisa Ceglia, Karl Olsson, Thomas Gustafsson, Alice Lichtenstein, Bess Dawson-Hughes, Roger Fielding] Vitamin D receptor (VDR) expression and action in non-human skeletal muscle have recently been reported in several studies, yet data on the activity and expression of VDR in human muscle cells are scarce. We conducted a series of studies to examine the (1) effect of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) on VDR gene expression in human primary myoblasts, (2) effect of 16-week supplementation with vitamin D3 on intramuscular VDR gene expression in older women, and (3) association between serum 25-hydroxyvitamin D (25OHD) and intramuscular VDR protein concentration in older adults. Human primary myoblasts were treated with increasing concentrations of 1,25(OH)2D3 for 18h. A dose-dependent treatment effect was noted with 1nmol/L of 1,25OH2D3 increasing intramuscular VDR mRNA expression (mean fold change±SD 1.36±0.33; P=0.05). Muscle biopsies were obtained at baseline and 16weeks after vitamin D3 supplementation (4,000IU/day) in older adults. Intramuscular VDR mRNA was significantly different from placebo after 16weeks of vitamin D3 (1.2±0.99; −3.2±1.7, respectively; P=0.04). Serum 25OHD and intramuscular VDR protein expression were examined by immunoblot. 25OHD was associated with intramuscular VDR protein concentration (R=0.67; P=0.0028). In summary, our study found VDR gene expression increases following treatment with 1,25OH2D3 in human myoblasts. 25OHD is associated with VDR protein and 16weeks of supplementation with vitamin D3 resulted in a persistent increase in VDR gene expression of vitamin D3 in muscle tissue biopsies. These findings suggest treatment with vitamin D compounds results in sustained increases in VDR in human skeletal muscle. Springer Science+Business Media New York, 2014 Vitamin D nationallicence Skeletal muscle nationallicence Vitamin D receptor nationallicence Human nationallicence Primary myoblasts nationallicence Aging nationallicence Pojednic Rachele Nutrition, Exercise Physiology and Sarcopenia Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, 711 Washington St., 02111, Boston, MA, USA aut Ceglia Lisa Division of Endocrinology, Diabetes and Metabolism, Tufts Medical Center, Boston, MA, USA aut Olsson Karl Division of Clinical Physiology, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden aut Gustafsson Thomas Division of Clinical Physiology, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden aut Lichtenstein Alice Cardiovascular Nutrition Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston, MA, USA aut Dawson-Hughes Bess Bone Metabolism Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston, MA, USA aut Fielding Roger Nutrition, Exercise Physiology and Sarcopenia Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, 711 Washington St., 02111, Boston, MA, USA aut Calcified Tissue International Springer US; http://www.springer-ny.com 96/3(2015-03-01), 256-263 0171-967X 96:3<256 2015 96 223 https://doi.org/10.1007/s00223-014-9932-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00223-014-9932-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pojednic Rachele Nutrition, Exercise Physiology and Sarcopenia Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, 711 Washington St., 02111, Boston, MA, USA aut NATIONALLICENCE 700 1- Ceglia Lisa Division of Endocrinology, Diabetes and Metabolism, Tufts Medical Center, Boston, MA, USA aut NATIONALLICENCE 700 1- Olsson Karl Division of Clinical Physiology, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden aut NATIONALLICENCE 700 1- Gustafsson Thomas Division of Clinical Physiology, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden aut NATIONALLICENCE 700 1- Lichtenstein Alice Cardiovascular Nutrition Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston, MA, USA aut NATIONALLICENCE 700 1- Dawson-Hughes Bess Bone Metabolism Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston, MA, USA aut NATIONALLICENCE 700 1- Fielding Roger Nutrition, Exercise Physiology and Sarcopenia Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, 711 Washington St., 02111, Boston, MA, USA aut NATIONALLICENCE 773 0- Calcified Tissue International Springer US; http://www.springer-ny.com 96/3(2015-03-01), 256-263 0171-967X 96:3<256 2015 96 223