caa a22 4500 605521387 CHVBK 20210128100739.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00223-015-9957-9 doi (NATIONALLICENCE)springer-10.1007/s00223-015-9957-9 Cortical Bone Histomorphometry in Male Femoral Neck: The Investigation of Age-Association and Regional Differences [Elektronische Daten] [Xiaoyu Tong, Inari Burton, Hanna Isaksson, Jukka Jurvelin, Heikki Kröger] Low bone volume and changes in bone quality or microarchitecture may predispose individuals to fragility fractures. As the dominant component of the human skeleton, cortical bone plays a key role in protecting bones from fracture. However, histological investigations of the underlying structural changes, which might predispose to fracture, have been largely limited to the cancellous bone. The aim of this study was to investigate the age-association and regional differences of histomorphometric properties in the femoral neck cortical bone. Undecalcified histological sections of the femoral neck (n=20, aged 18-82years, males) were cut (15μm) and stained using modified Masson-Goldner stain. Complete femoral neck images were scanned, and cortical bone boundaries were defined using our previously established method. Cortical bone histomorphometry was performed with low (×50) and high magnification (×100). Most parameters related to cortical width (Mean Ct.Wi, Inferior Ct.Wi, Superior Ct.Wi) were negatively associated with age both before and after adjustment for height. The inferior cortex was the thickest (P<0.001) and the superior cortex was the thinnest (P<0.008) of all cortical regions. Both osteonal size and pores area were negatively associated with age. Osteonal area and number were higher in the antero-inferior area (P<0.002) and infero-posterior area (P=0.002) compared to the postero-superior area. The Haversian canal area was higher in the infero-posterior area compared to the postero-superior area (P=0.002). Moreover, porosity was higher in the antero-superior area (P<0.002), supero-anterior area (P<0.002) and supero-posterior area (P<0.002) compared to the infero-anterior area. Eroded endocortical perimeter (E.Pm/Ec.Pm) correlated positively with superior cortical width. This study describes the changes in cortical bone during ageing in healthy males. Further studies are needed to investigate whether these changes explain the increased susceptibility to femoral neck fractures. Springer Science+Business Media New York, 2015 Bone histomorphometry nationallicence Age-association nationallicence Femoral neck nationallicence Osteoporosis nationallicence Tong Xiaoyu Bone and Cartilage Research Unit (BCRU), Institute of Clinical Medicine, University of Eastern Finland, POB 1627, 70211, Kuopio, Finland aut Burton Inari Bone and Cartilage Research Unit (BCRU), Institute of Clinical Medicine, University of Eastern Finland, POB 1627, 70211, Kuopio, Finland aut Isaksson Hanna Department of Biomedical Engineering, Department of Orthopaedics, Lund University, POB 118, 221 00, Lund, Sweden aut Jurvelin Jukka Department of Applied Physics, University of Eastern Finland, POB 1627, 70211, Kuopio, Finland aut Kröger Heikki Bone and Cartilage Research Unit (BCRU), Institute of Clinical Medicine, University of Eastern Finland, POB 1627, 70211, Kuopio, Finland aut Calcified Tissue International Springer US; http://www.springer-ny.com 96/4(2015-04-01), 295-306 0171-967X 96:4<295 2015 96 223 https://doi.org/10.1007/s00223-015-9957-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00223-015-9957-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tong Xiaoyu Bone and Cartilage Research Unit (BCRU), Institute of Clinical Medicine, University of Eastern Finland, POB 1627, 70211, Kuopio, Finland aut NATIONALLICENCE 700 1- Burton Inari Bone and Cartilage Research Unit (BCRU), Institute of Clinical Medicine, University of Eastern Finland, POB 1627, 70211, Kuopio, Finland aut NATIONALLICENCE 700 1- Isaksson Hanna Department of Biomedical Engineering, Department of Orthopaedics, Lund University, POB 118, 221 00, Lund, Sweden aut NATIONALLICENCE 700 1- Jurvelin Jukka Department of Applied Physics, University of Eastern Finland, POB 1627, 70211, Kuopio, Finland aut NATIONALLICENCE 700 1- Kröger Heikki Bone and Cartilage Research Unit (BCRU), Institute of Clinical Medicine, University of Eastern Finland, POB 1627, 70211, Kuopio, Finland aut NATIONALLICENCE 773 0- Calcified Tissue International Springer US; http://www.springer-ny.com 96/4(2015-04-01), 295-306 0171-967X 96:4<295 2015 96 223