caa a22 4500 605521476 CHVBK 20210128100740.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00223-015-9970-z doi (NATIONALLICENCE)springer-10.1007/s00223-015-9970-z Development of a Cyclosporin-A-Induced Immune Tolerant Rat Model to Test Marrow Allograft Cell Type Effects on Bone Repair [Elektronische Daten] [Florent Espitalier, Nicolas Durand, Séverine Rémy, Pierre Corre, Sophie Sourice, Paul Pilet, Pierre Weiss, Jérôme Guicheux, Olivier Malard] Bone repair is an important concept in tissue engineering, and the ability to repair bone in hypotrophic conditions such as that of irradiated bone, represents a challenge for this field. Previous studies have shown that a combination of bone marrow and (BCP) was effective to repair irradiated bone. However, the origin and role played by each cell type in bone healing still remains unclear. In order to track the grafted cells, the development of an animal model that is immunotolerant to an allograft of bone marrow would be useful. Furthermore, because the immune system interacts with bone turnover, it is of critical importance to demonstrate that immunosuppressive drugs do not interfere with bone repair. After a preliminary study of immunotolerance, cyclosporin-A was chosen to be used in immunosuppressive therapy. Ten rats were included to observe qualitative and quantitative bone repair 8days and 6weeks after the creation of bone defects. The defects were filled with an allograft of bone marrow alone or in association with BCP under immunosuppressive treatment (cyclosporin-A). The results showed that there was no significant interaction of cyclosporin-A with osseous regeneration. The use of this new immunotolerant rat model of bone marrow allograft in future studies will provide insight on how the cells within the bone marrow graft contribute to bone healing, especially in irradiated conditions. Springer Science+Business Media New York, 2015 Bone repair nationallicence Bone marrow allograft nationallicence Cyclosporin-A nationallicence Immunotolerance nationallicence Cell tracking nationallicence Espitalier Florent INSERM, UMRS 791, Laboratoire d'ingénierie ostéo-articulaire et dentaire, LIOAD, 1 place Alexis Ricordeau, 44042, Nantes Cedex 1, France aut Durand Nicolas INSERM, UMRS 791, Laboratoire d'ingénierie ostéo-articulaire et dentaire, LIOAD, 1 place Alexis Ricordeau, 44042, Nantes Cedex 1, France aut Rémy Séverine INSERM, U643, 30 Boulevard Jean Monnot, 44093, Nantes Cedex 1, France aut Corre Pierre INSERM, UMRS 791, Laboratoire d'ingénierie ostéo-articulaire et dentaire, LIOAD, 1 place Alexis Ricordeau, 44042, Nantes Cedex 1, France aut Sourice Sophie INSERM, UMRS 791, Laboratoire d'ingénierie ostéo-articulaire et dentaire, LIOAD, 1 place Alexis Ricordeau, 44042, Nantes Cedex 1, France aut Pilet Paul INSERM, UMRS 791, Laboratoire d'ingénierie ostéo-articulaire et dentaire, LIOAD, 1 place Alexis Ricordeau, 44042, Nantes Cedex 1, France aut Weiss Pierre INSERM, UMRS 791, Laboratoire d'ingénierie ostéo-articulaire et dentaire, LIOAD, 1 place Alexis Ricordeau, 44042, Nantes Cedex 1, France aut Guicheux Jérôme INSERM, UMRS 791, Laboratoire d'ingénierie ostéo-articulaire et dentaire, LIOAD, 1 place Alexis Ricordeau, 44042, Nantes Cedex 1, France aut Malard Olivier INSERM, UMRS 791, Laboratoire d'ingénierie ostéo-articulaire et dentaire, LIOAD, 1 place Alexis Ricordeau, 44042, Nantes Cedex 1, France aut Calcified Tissue International Springer US; http://www.springer-ny.com 96/5(2015-05-01), 430-437 0171-967X 96:5<430 2015 96 223 https://doi.org/10.1007/s00223-015-9970-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00223-015-9970-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Espitalier Florent INSERM, UMRS 791, Laboratoire d'ingénierie ostéo-articulaire et dentaire, LIOAD, 1 place Alexis Ricordeau, 44042, Nantes Cedex 1, France aut NATIONALLICENCE 700 1- Durand Nicolas INSERM, UMRS 791, Laboratoire d'ingénierie ostéo-articulaire et dentaire, LIOAD, 1 place Alexis Ricordeau, 44042, Nantes Cedex 1, France aut NATIONALLICENCE 700 1- Rémy Séverine INSERM, U643, 30 Boulevard Jean Monnot, 44093, Nantes Cedex 1, France aut NATIONALLICENCE 700 1- Corre Pierre INSERM, UMRS 791, Laboratoire d'ingénierie ostéo-articulaire et dentaire, LIOAD, 1 place Alexis Ricordeau, 44042, Nantes Cedex 1, France aut NATIONALLICENCE 700 1- Sourice Sophie INSERM, UMRS 791, Laboratoire d'ingénierie ostéo-articulaire et dentaire, LIOAD, 1 place Alexis Ricordeau, 44042, Nantes Cedex 1, France aut NATIONALLICENCE 700 1- Pilet Paul INSERM, UMRS 791, Laboratoire d'ingénierie ostéo-articulaire et dentaire, LIOAD, 1 place Alexis Ricordeau, 44042, Nantes Cedex 1, France aut NATIONALLICENCE 700 1- Weiss Pierre INSERM, UMRS 791, Laboratoire d'ingénierie ostéo-articulaire et dentaire, LIOAD, 1 place Alexis Ricordeau, 44042, Nantes Cedex 1, France aut NATIONALLICENCE 700 1- Guicheux Jérôme INSERM, UMRS 791, Laboratoire d'ingénierie ostéo-articulaire et dentaire, LIOAD, 1 place Alexis Ricordeau, 44042, Nantes Cedex 1, France aut NATIONALLICENCE 700 1- Malard Olivier INSERM, UMRS 791, Laboratoire d'ingénierie ostéo-articulaire et dentaire, LIOAD, 1 place Alexis Ricordeau, 44042, Nantes Cedex 1, France aut NATIONALLICENCE 773 0- Calcified Tissue International Springer US; http://www.springer-ny.com 96/5(2015-05-01), 430-437 0171-967X 96:5<430 2015 96 223