caa a22 4500 605521484 CHVBK 20210128100740.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00223-015-9973-9 doi (NATIONALLICENCE)springer-10.1007/s00223-015-9973-9 The Generation of C-3α Epimer of 25-Hydroxyvitamin D and Its Biological Effects on Bone Mineral Density in Adult Rodents [Elektronische Daten] [Christina Bianchini, Paula Lavery, Sherry Agellon, Hope Weiler] The source and function of C-3α epimer of 25(OH)D (C-3 epimer) is unknown. The objectives were to (1) establish if increasing doses of vitamin D (VD) results in a proportionate dose-response in C-3 epimer; and (2) determine the biological response of bone to C-3 epimer treatment. Sprague Dawley rats (12weeks, n=36 female, n=36 male) were randomized to control AIN93-M diet (1IU VD3/g diet) or an experimental diet for 8weeks containing VD3 at 2 or 4IU/g diet, C-3 epimer at 0.5 or 1IU/g diet or 25(OH)D (0.5IU/g diet). BW and food consumption were measured weekly. Blood was sampled at week 0, 4, and 8 for assessment of VD metabolites and bone metabolism biomarkers. DXA (week 0, 4, and 8) and in vivo micro CT (μCT) (week 0 and 8) were performed in vivo plus ex vivo μCT imaging and bone biomechanics. Dietary intake and anthropometry did not differ among diet groups. The dose-response of VD generated significantly elevated C-3 epimer only in females with concentrations of 4IU VD diet group [mean 84.6 (62.5)nmol/L] exceeding control [mean 21.4 (18.5)nmol/L, p=0.005]. Both sexes in the 25(OH)D group did not show significant increases in C-3 epimer, whereas 0.5 and 1IU epimer groups exceeded 100nmol/L of C-3 epimer by 8weeks. These data suggest C-3 epimer is endogenously generated with higher intakes of VD. Endogenous and exogenous C-3 epimer accumulates in serum without impact upon bone health outcomes in a healthy young adult model over 8weeks. Springer Science+Business Media New York, 2015 Vitamin D nationallicence 3-epi-25(OH)D nationallicence Cholecalciferol nationallicence Bone nationallicence Rodents nationallicence Bianchini Christina Dietetics and Human Nutrition, McGill University, Macdonald-Stewart Building, Macdonald Campus, 21111 Lakeshore Road, Ste-Anne-de-Bellevue, H9X 3V9, Montréal, QC, Canada aut Lavery Paula Dietetics and Human Nutrition, McGill University, Macdonald-Stewart Building, Macdonald Campus, 21111 Lakeshore Road, Ste-Anne-de-Bellevue, H9X 3V9, Montréal, QC, Canada aut Agellon Sherry Dietetics and Human Nutrition, McGill University, Macdonald-Stewart Building, Macdonald Campus, 21111 Lakeshore Road, Ste-Anne-de-Bellevue, H9X 3V9, Montréal, QC, Canada aut Weiler Hope Dietetics and Human Nutrition, McGill University, Macdonald-Stewart Building, Macdonald Campus, 21111 Lakeshore Road, Ste-Anne-de-Bellevue, H9X 3V9, Montréal, QC, Canada aut Calcified Tissue International Springer US; http://www.springer-ny.com 96/5(2015-05-01), 453-464 0171-967X 96:5<453 2015 96 223 https://doi.org/10.1007/s00223-015-9973-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00223-015-9973-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bianchini Christina Dietetics and Human Nutrition, McGill University, Macdonald-Stewart Building, Macdonald Campus, 21111 Lakeshore Road, Ste-Anne-de-Bellevue, H9X 3V9, Montréal, QC, Canada aut NATIONALLICENCE 700 1- Lavery Paula Dietetics and Human Nutrition, McGill University, Macdonald-Stewart Building, Macdonald Campus, 21111 Lakeshore Road, Ste-Anne-de-Bellevue, H9X 3V9, Montréal, QC, Canada aut NATIONALLICENCE 700 1- Agellon Sherry Dietetics and Human Nutrition, McGill University, Macdonald-Stewart Building, Macdonald Campus, 21111 Lakeshore Road, Ste-Anne-de-Bellevue, H9X 3V9, Montréal, QC, Canada aut NATIONALLICENCE 700 1- Weiler Hope Dietetics and Human Nutrition, McGill University, Macdonald-Stewart Building, Macdonald Campus, 21111 Lakeshore Road, Ste-Anne-de-Bellevue, H9X 3V9, Montréal, QC, Canada aut NATIONALLICENCE 773 0- Calcified Tissue International Springer US; http://www.springer-ny.com 96/5(2015-05-01), 453-464 0171-967X 96:5<453 2015 96 223