Deteriorating Effect on Bone Metabolism and Microstructure by Passive Cigarette Smoking Through Dual Actions on Osteoblast and Osteoclast
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| 024 | 7 | 0 | |a 10.1007/s00223-015-9966-8 |2 doi |
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| 245 | 0 | 0 | |a Deteriorating Effect on Bone Metabolism and Microstructure by Passive Cigarette Smoking Through Dual Actions on Osteoblast and Osteoclast |h [Elektronische Daten] |c [Chun Ko, Ruby Chan, Wing Siu, Wai Shum, Ping Leung, Lin Zhang, Chi Cho] |
| 520 | 3 | |a There is no clear evidence to show the direct causal relationship between passive cigarette smoking and osteoporosis. Furthermore, the underlying mechanism is unknown. The objective of this study is to demonstrate the effects of long-term passive cigarette smoking on bone metabolism and microstructure by a mouse model and cell culture systems. BALB/c mice were exposed to 2 or 4% cigarette smoke for 14weeks. The bone turnover biochemical markers in urine and serum and also the bone micro-architecture by micro-CT were compared with the control group exposed to normal ambient air. In the cell culture experiments, mouse MC3T3-E1 and RAW264.7 cell lines to be employed as osteoblast and osteoclast, respectively, were treated with the sera obtained from 4% smoking or control mice. Their actions on cell viability, differentiation, and function on these bone cells were assessed. The urinary mineral and deoxypyridinoline(DPD) levels, and also the serum alkaline phosphatase activity, were significantly higher in the 4% smoking group when compared with the control group, indicating an elevated bone metabolism after cigarette smoking. In addition, femoral osteopenic condition was observed in the 4% smoking group, as shown by the decrease of relative bone volume and trabecular thickness. In isolated cell studies, osteoblast differentiation and bone formation were inhibited while osteoclast differentiation was increased. The current mouse smoking model and the isolated cell studies demonstrate that passive cigarette smoke could induce osteopenia by exerting a direct detrimental effect on bone cells differentiation and further on bone remodeling process. | |
| 540 | |a Springer Science+Business Media New York, 2015 | ||
| 690 | 7 | |a Cigarette smoking |2 nationallicence | |
| 690 | 7 | |a Osteopenia |2 nationallicence | |
| 690 | 7 | |a Mouse passive smoking model |2 nationallicence | |
| 690 | 7 | |a Osteoblast differentiation |2 nationallicence | |
| 690 | 7 | |a Osteoclast differentiation |2 nationallicence | |
| 690 | 7 | |a ALP : Alkaline phosphatase |2 nationallicence | |
| 690 | 7 | |a ALT : Alanine transaminase |2 nationallicence | |
| 690 | 7 | |a AST : Aspartate aminotransferase |2 nationallicence | |
| 690 | 7 | |a BMD : Bone mineral density |2 nationallicence | |
| 690 | 7 | |a BV/TV : Bone volume per tissue volume |2 nationallicence | |
| 690 | 7 | |a Ca : Calcium |2 nationallicence | |
| 690 | 7 | |a Cr : Creatinine |2 nationallicence | |
| 690 | 7 | |a DPD : Deoxypyridinoline |2 nationallicence | |
| 690 | 7 | |a μCT : Micro-computed tomography |2 nationallicence | |
| 690 | 7 | |a MTT : 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide |2 nationallicence | |
| 690 | 7 | |a P : Phosphorus |2 nationallicence | |
| 690 | 7 | |a SMI : Structural model index |2 nationallicence | |
| 690 | 7 | |a Tb. N. : Trabecular number |2 nationallicence | |
| 690 | 7 | |a Tb. Sp. : Trabecular spacing |2 nationallicence | |
| 690 | 7 | |a Tb. Th. : Trabecular thickness |2 nationallicence | |
| 690 | 7 | |a TRAP : Tartrate-resistant acid phosphatase |2 nationallicence | |
| 700 | 1 | |a Ko |D Chun |u Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China |4 aut | |
| 700 | 1 | |a Chan |D Ruby |u School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China |4 aut | |
| 700 | 1 | |a Siu |D Wing |u Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China |4 aut | |
| 700 | 1 | |a Shum |D Wai |u Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China |4 aut | |
| 700 | 1 | |a Leung |D Ping |u Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China |4 aut | |
| 700 | 1 | |a Zhang |D Lin |u School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China |4 aut | |
| 700 | 1 | |a Cho |D Chi |u School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China |4 aut | |
| 773 | 0 | |t Calcified Tissue International |d Springer US; http://www.springer-ny.com |g 96/5(2015-05-01), 389-400 |x 0171-967X |q 96:5<389 |1 2015 |2 96 |o 223 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00223-015-9966-8 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00223-015-9966-8 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ko |D Chun |u Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Chan |D Ruby |u School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Siu |D Wing |u Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Shum |D Wai |u Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Leung |D Ping |u Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhang |D Lin |u School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Cho |D Chi |u School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Calcified Tissue International |d Springer US; http://www.springer-ny.com |g 96/5(2015-05-01), 389-400 |x 0171-967X |q 96:5<389 |1 2015 |2 96 |o 223 | ||