caa a22 4500 605521530 CHVBK 20210128100740.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00223-015-9969-5 doi (NATIONALLICENCE)springer-10.1007/s00223-015-9969-5 Dickkopf-1 and Sclerostin Serum Levels in Patients with Systemic Mastocytosis [Elektronische Daten] [Maurizio Rossini, Ombretta Viapiana, Roberta Zanotti, Gaia Tripi, Omar Perbellini, Luca Idolazzi, Massimiliano Bonifacio, Silvano Adami, Davide Gatti] Bone involvement, mainly osteoporosis but also osteosclerosis, is frequent in patients with indolent systemic mastocytosis (ISM). The recent characterization of the canonical Wnt/β-catenin pathway in the regulation of bone remodeling provided important insights for our understanding of the pathophysiology of a number of conditions. The regulation of Wnt pathway in bone is predominantly driven by the production of receptor inhibitors such as Dickkopf-1 (DKK1) and sclerostin (SOST). This study aimed to explore if the various bone involvements in patients with ISM might be explained by variations in serum levels of DKK1 and SOST. This is a cross-sectional study in an adult ISM cohort (13 men and 13 women with diagnosed ISM) and fifty-two healthy sex and age-matched controls. Early morning, fasting and venous sampling was obtained in all subjects. The main outcome measures were serum bone-specific alkaline phosphatase (bALP), C-terminal telopeptides of type I collagene (CTX), DKK1, SOST, parathyroid hormone (PTH), bone mineral density, and prevalent vertebral fractures. Mean DKK1 serum levels were about two-folds higher in patients, than in controls (65,0±43.3 vs. 33.1±19.4pmol/L, respectively; p<0.001), irrespective of the presence of osteoporotic or diffuse osteosclerotic bone involvement. DKK1 serum levels were positively correlated with PTH and both CTX and bALP. Mean SOST serum levels were not significantly different in patients versus controls, and we did not observe any significant correlation between SOST and any available clinical or laboratory parameters, with the only exception of a positive correlation with age. In conclusion, in our study, we observed that DKK1, but not SOST, serum levels significantly increased in ISM patients with various bone involvements, and correlated with PTH and bone turnover markers. Our results suggest that the Wnt/β-catenin pathway is not primarily involved in the pathophysiology of the array of bone involvement in ISM. Springer Science+Business Media New York, 2015 Mastocytosis nationallicence DKK1 nationallicence Sclerostin nationallicence Wnt nationallicence Osteoporosis nationallicence Osteosclerosis nationallicence Rossini Maurizio Rheumatology Section, Department of Medicine, University of Verona, Policlinico Borgo Roma, Piazzale Scuro, 10, 37134, Verona, Italy aut Viapiana Ombretta Rheumatology Section, Department of Medicine, University of Verona, Policlinico Borgo Roma, Piazzale Scuro, 10, 37134, Verona, Italy aut Zanotti Roberta Hematology Section, Department of Medicine, University of Verona, Verona, Italy aut Tripi Gaia Rheumatology Section, Department of Medicine, University of Verona, Policlinico Borgo Roma, Piazzale Scuro, 10, 37134, Verona, Italy aut Perbellini Omar Hematology Section, Department of Medicine, University of Verona, Verona, Italy aut Idolazzi Luca Rheumatology Section, Department of Medicine, University of Verona, Policlinico Borgo Roma, Piazzale Scuro, 10, 37134, Verona, Italy aut Bonifacio Massimiliano Hematology Section, Department of Medicine, University of Verona, Verona, Italy aut Adami Silvano Rheumatology Section, Department of Medicine, University of Verona, Policlinico Borgo Roma, Piazzale Scuro, 10, 37134, Verona, Italy aut Gatti Davide Rheumatology Section, Department of Medicine, University of Verona, Policlinico Borgo Roma, Piazzale Scuro, 10, 37134, Verona, Italy aut Calcified Tissue International Springer US; http://www.springer-ny.com 96/5(2015-05-01), 410-416 0171-967X 96:5<410 2015 96 223 https://doi.org/10.1007/s00223-015-9969-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00223-015-9969-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rossini Maurizio Rheumatology Section, Department of Medicine, University of Verona, Policlinico Borgo Roma, Piazzale Scuro, 10, 37134, Verona, Italy aut NATIONALLICENCE 700 1- Viapiana Ombretta Rheumatology Section, Department of Medicine, University of Verona, Policlinico Borgo Roma, Piazzale Scuro, 10, 37134, Verona, Italy aut NATIONALLICENCE 700 1- Zanotti Roberta Hematology Section, Department of Medicine, University of Verona, Verona, Italy aut NATIONALLICENCE 700 1- Tripi Gaia Rheumatology Section, Department of Medicine, University of Verona, Policlinico Borgo Roma, Piazzale Scuro, 10, 37134, Verona, Italy aut NATIONALLICENCE 700 1- Perbellini Omar Hematology Section, Department of Medicine, University of Verona, Verona, Italy aut NATIONALLICENCE 700 1- Idolazzi Luca Rheumatology Section, Department of Medicine, University of Verona, Policlinico Borgo Roma, Piazzale Scuro, 10, 37134, Verona, Italy aut NATIONALLICENCE 700 1- Bonifacio Massimiliano Hematology Section, Department of Medicine, University of Verona, Verona, Italy aut NATIONALLICENCE 700 1- Adami Silvano Rheumatology Section, Department of Medicine, University of Verona, Policlinico Borgo Roma, Piazzale Scuro, 10, 37134, Verona, Italy aut NATIONALLICENCE 700 1- Gatti Davide Rheumatology Section, Department of Medicine, University of Verona, Policlinico Borgo Roma, Piazzale Scuro, 10, 37134, Verona, Italy aut NATIONALLICENCE 773 0- Calcified Tissue International Springer US; http://www.springer-ny.com 96/5(2015-05-01), 410-416 0171-967X 96:5<410 2015 96 223