caa a22 4500 605521565 CHVBK 20210128100741.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00223-014-9945-5 doi (NATIONALLICENCE)springer-10.1007/s00223-014-9945-5 Osterix-Cre Transgene Causes Craniofacial Bone Development Defect [Elektronische Daten] [Li Wang, Yuji Mishina, Fei Liu] The Cre/loxP system has been widely used to generate tissue-specific gene knockout mice. Inducible (Tet-off) Osx-GFP::Cre (Osx-Cre) mouse line that targets osteoblasts is widely used in the bone research field. In this study, we investigated the effect of Osx-Cre on craniofacial bone development. We found that newborn Osx-Cre mice showed severe hypomineralization in parietal, frontal, and nasal bones as well as the coronal sutural area when compared to control mice. As the mice matured, the intramembranous bone hypomineralization phenotype became less severe. The major hypomineralization defect in parietal, frontal, and nasal bones had mostly disappeared by postnatal day 21, but the defect in sutural areas persisted. Importantly, Doxycycline treatment eliminated cranial bone defects at birth which indicates that Cre expression may be responsible for the phenotype. In addition, we showed that the primary calvarial osteoblasts isolated from neonatal Osx-Cre mice had comparable differentiation ability compared to their littermate controls. This study reinforces the idea that Cre-positive litter mates are indispensable controls in studies using conditional gene deletion. Springer Science+Business Media New York, 2014 Osx -Cre nationallicence Intramembranous bone nationallicence Craniofacial nationallicence Suture nationallicence Hypomineralization nationallicence Wang Li Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, 48109, Ann Arbor, MI, USA aut Mishina Yuji Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, 48109, Ann Arbor, MI, USA aut Liu Fei Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, 48109, Ann Arbor, MI, USA aut Calcified Tissue International Springer US; http://www.springer-ny.com 96/2(2015-02-01), 129-137 0171-967X 96:2<129 2015 96 223 https://doi.org/10.1007/s00223-014-9945-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00223-014-9945-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Li Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, 48109, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Mishina Yuji Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, 48109, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Liu Fei Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, 48109, Ann Arbor, MI, USA aut NATIONALLICENCE 773 0- Calcified Tissue International Springer US; http://www.springer-ny.com 96/2(2015-02-01), 129-137 0171-967X 96:2<129 2015 96 223