caa a22 4500 605521611 CHVBK 20210128100741.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00223-014-9942-8 doi (NATIONALLICENCE)springer-10.1007/s00223-014-9942-8 Effects of Adding Chymosin to Milk on Calcium Homeostasis: A Randomized, Double-Blind, Cross-Over Study [Elektronische Daten] [Ulla Møller, Lars Jensen, Leif Mosekilde, Lars Rejnmark] Calcium intake and absorption is important for bone health. In a randomized double-blind cross-over trial, we investigated effects of adding chymosin to milk on the intestinal calcium absorption as measured by renal calcium excretion and indices of calcium homeostasis. The primary outcome of the study was 24-h renal calcium excretion that is considered a proxy measure of the amount of calcium absorbed from the intestine. We studied 125 healthy men and women, aged 34 (25-45)years on two separate days. On each day, a light breakfast was served together with 500ml of semi-skimmed milk to which either chymosin or similar placebo was added. Compared with placebo, chymosin did not affect 24-h urinary calcium, calcium/creatinine ratio, plasma parathyroid hormone, calcitonin or ionized calcium levels. However, during the first 4h after intake of milk with chymosin, urinary calcium-creatinine ratio was significantly increased (17%) compared with placebo. Stratification by daily calcium intake showed effect of chymosin in participant with a habitual intake above the median (>1,050mg/day) in whom both urinary calcium and calcium/creatinine ratio were significantly increased compared with placebo. Effects did not depend on plasma 25-hydroxyvitamin D levels. Chymosin added to milk increases renal calcium excretion in the hours following intake without affecting plasma levels of calcium or calciotropic hormones. The effect most likely represents enhanced intestinal calcium absorption shortly after intake. Further studies are warranted on whether intake of milk-added chymosin may cause beneficial effects on bone. www.ClinicalTrials.gov no. NCT01370941. Springer Science+Business Media New York, 2014 Chymosin nationallicence Calcium nationallicence Intestinal absorption nationallicence Renal excretion nationallicence Møller Ulla Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Tage-Hansens Gade 2, 8000, Aarhus C, Denmark aut Jensen Lars Department of Clinical Physiology, Glostrup University Hospital, Copenhagen, Denmark aut Mosekilde Leif Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Tage-Hansens Gade 2, 8000, Aarhus C, Denmark aut Rejnmark Lars Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Tage-Hansens Gade 2, 8000, Aarhus C, Denmark aut Calcified Tissue International Springer US; http://www.springer-ny.com 96/2(2015-02-01), 105-112 0171-967X 96:2<105 2015 96 223 https://doi.org/10.1007/s00223-014-9942-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00223-014-9942-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Møller Ulla Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Tage-Hansens Gade 2, 8000, Aarhus C, Denmark aut NATIONALLICENCE 700 1- Jensen Lars Department of Clinical Physiology, Glostrup University Hospital, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Mosekilde Leif Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Tage-Hansens Gade 2, 8000, Aarhus C, Denmark aut NATIONALLICENCE 700 1- Rejnmark Lars Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Tage-Hansens Gade 2, 8000, Aarhus C, Denmark aut NATIONALLICENCE 773 0- Calcified Tissue International Springer US; http://www.springer-ny.com 96/2(2015-02-01), 105-112 0171-967X 96:2<105 2015 96 223