The peril of PCR inhibitors in environmental samples: the case of Didymosphenia geminata
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| 024 | 7 | 0 | |a 10.1007/s10531-014-0849-5 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10531-014-0849-5 | ||
| 245 | 0 | 4 | |a The peril of PCR inhibitors in environmental samples: the case of Didymosphenia geminata |h [Elektronische Daten] |c [Leandro Jones, Noelia Uyua, Julieta Manrique] |
| 520 | 3 | |a Since the introduction of polymerase chain reaction (PCR) biodiversity study has been significantly influenced by the chance of generating unprecedented amounts of molecular data. Although it is a robust technique, those applications requiring high sensitivity and reproducibility, that is PCR detection and quantitative PCR, can be negatively affected by PCR inhibition. This is particularly challenging for diverse kinds of samples included the environmental ones, which usually contain complex mixtures of a variety of inhibitory substances. The problem of PCR inhibition can be overcome, or ameliorated, by implementing adequate protocols of nucleic acids purification, internal controls and modern analytical approaches focused on PCR kinetics. Herein, we remark these procedures and describe the general techniques that can be used to optimize DNA extraction protocols for PCR applications. In addition, we show that PCR inhibition might have negative consequences in molecular studies of Didymosphenia geminata, an invasive microalga that have recently developed massive blooms in temperate regions worldwide, and provide general guidelines for dealing with this problem. | |
| 540 | |a Springer Science+Business Media Dordrecht, 2014 | ||
| 690 | 7 | |a Didymosphenia |2 nationallicence | |
| 690 | 7 | |a Rock snot |2 nationallicence | |
| 690 | 7 | |a Molecular detection |2 nationallicence | |
| 690 | 7 | |a PCR |2 nationallicence | |
| 700 | 1 | |a Jones |D Leandro |u Laboratorio de Virología y Genética Molecular, Facultad de Ciencias Naturales sede Trelew, Universidad Nacional de la Patagonia San Juan Bosco, Trelew, Chubut, Argentina |4 aut | |
| 700 | 1 | |a Uyua |D Noelia |u Laboratorio de Virología y Genética Molecular, Facultad de Ciencias Naturales sede Trelew, Universidad Nacional de la Patagonia San Juan Bosco, Trelew, Chubut, Argentina |4 aut | |
| 700 | 1 | |a Manrique |D Julieta |u Laboratorio de Virología y Genética Molecular, Facultad de Ciencias Naturales sede Trelew, Universidad Nacional de la Patagonia San Juan Bosco, Trelew, Chubut, Argentina |4 aut | |
| 773 | 0 | |t Biodiversity and Conservation |d Springer Netherlands |g 24/6(2015-06-01), 1541-1548 |x 0960-3115 |q 24:6<1541 |1 2015 |2 24 |o 10531 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10531-014-0849-5 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10531-014-0849-5 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Jones |D Leandro |u Laboratorio de Virología y Genética Molecular, Facultad de Ciencias Naturales sede Trelew, Universidad Nacional de la Patagonia San Juan Bosco, Trelew, Chubut, Argentina |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Uyua |D Noelia |u Laboratorio de Virología y Genética Molecular, Facultad de Ciencias Naturales sede Trelew, Universidad Nacional de la Patagonia San Juan Bosco, Trelew, Chubut, Argentina |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Manrique |D Julieta |u Laboratorio de Virología y Genética Molecular, Facultad de Ciencias Naturales sede Trelew, Universidad Nacional de la Patagonia San Juan Bosco, Trelew, Chubut, Argentina |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biodiversity and Conservation |d Springer Netherlands |g 24/6(2015-06-01), 1541-1548 |x 0960-3115 |q 24:6<1541 |1 2015 |2 24 |o 10531 | ||