caa a22 4500 605526478 CHVBK 20210128100803.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10531-014-0849-5 doi (NATIONALLICENCE)springer-10.1007/s10531-014-0849-5 The peril of PCR inhibitors in environmental samples: the case of Didymosphenia geminata [Elektronische Daten] [Leandro Jones, Noelia Uyua, Julieta Manrique] Since the introduction of polymerase chain reaction (PCR) biodiversity study has been significantly influenced by the chance of generating unprecedented amounts of molecular data. Although it is a robust technique, those applications requiring high sensitivity and reproducibility, that is PCR detection and quantitative PCR, can be negatively affected by PCR inhibition. This is particularly challenging for diverse kinds of samples included the environmental ones, which usually contain complex mixtures of a variety of inhibitory substances. The problem of PCR inhibition can be overcome, or ameliorated, by implementing adequate protocols of nucleic acids purification, internal controls and modern analytical approaches focused on PCR kinetics. Herein, we remark these procedures and describe the general techniques that can be used to optimize DNA extraction protocols for PCR applications. In addition, we show that PCR inhibition might have negative consequences in molecular studies of Didymosphenia geminata, an invasive microalga that have recently developed massive blooms in temperate regions worldwide, and provide general guidelines for dealing with this problem. Springer Science+Business Media Dordrecht, 2014 Didymosphenia nationallicence Rock snot nationallicence Molecular detection nationallicence PCR nationallicence Jones Leandro Laboratorio de Virología y Genética Molecular, Facultad de Ciencias Naturales sede Trelew, Universidad Nacional de la Patagonia San Juan Bosco, Trelew, Chubut, Argentina aut Uyua Noelia Laboratorio de Virología y Genética Molecular, Facultad de Ciencias Naturales sede Trelew, Universidad Nacional de la Patagonia San Juan Bosco, Trelew, Chubut, Argentina aut Manrique Julieta Laboratorio de Virología y Genética Molecular, Facultad de Ciencias Naturales sede Trelew, Universidad Nacional de la Patagonia San Juan Bosco, Trelew, Chubut, Argentina aut Biodiversity and Conservation Springer Netherlands 24/6(2015-06-01), 1541-1548 0960-3115 24:6<1541 2015 24 10531 https://doi.org/10.1007/s10531-014-0849-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10531-014-0849-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jones Leandro Laboratorio de Virología y Genética Molecular, Facultad de Ciencias Naturales sede Trelew, Universidad Nacional de la Patagonia San Juan Bosco, Trelew, Chubut, Argentina aut NATIONALLICENCE 700 1- Uyua Noelia Laboratorio de Virología y Genética Molecular, Facultad de Ciencias Naturales sede Trelew, Universidad Nacional de la Patagonia San Juan Bosco, Trelew, Chubut, Argentina aut NATIONALLICENCE 700 1- Manrique Julieta Laboratorio de Virología y Genética Molecular, Facultad de Ciencias Naturales sede Trelew, Universidad Nacional de la Patagonia San Juan Bosco, Trelew, Chubut, Argentina aut NATIONALLICENCE 773 0- Biodiversity and Conservation Springer Netherlands 24/6(2015-06-01), 1541-1548 0960-3115 24:6<1541 2015 24 10531