caa a22 4500 605534659 CHVBK 20210128100843.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s10517-015-2911-z doi (NATIONALLICENCE)springer-10.1007/s10517-015-2911-z Effect of Monocyte-Like THP-1 Cells on the Formation of Vascular Tubes by EA.Hy926s Endothelial Cells in the Presence of Cytokines [Elektronische Daten] [T. L'vova, O. Stepanova, L. Okorokova, V. Semenov, E. Rebezova, S. Sel'kov, D. Sokolov] The interaction of endothelial cells with cells of the microenvironment, including monocytes/ macrophages, and extracellular matrix during angiogenesis is controlled by cytokines. The stimulating effect bFGF, IL-8, and VEGF on the formation of capillary-like structures by endothelial cells was demonstrated in both monoculture and in co-culture with THP-1 cells; in the latter case, the effects of bFGF and VEGF were more pronounced. IL-8 reduced branching of vascular tubes in co-culture in comparison with monoculture of endothelial cells. Placental growth factor PlGF had no effect of tube formation by endothelial cells in monoculture, but in co-culture with THP-1 cells this cytokine in high concentrations exhibited proangiogenic activity. TGFb inhibited the formation of vascular tubes by endothelial cells and its antiangiogenic potential was more pronounced in co-culture with THP-1 cells. Springer Science+Business Media New York, 2015 angiogenesis nationallicence endothelial cells nationallicence cytokines nationallicence monocytes nationallicence macrophages nationallicence L'vova T. D. O. Ott Research Institute of Obstetrics and Gynecology, North-Western Division of the Russian Academy of Medical Sciences, St. Petersburg, Russia aut Stepanova O. D. O. Ott Research Institute of Obstetrics and Gynecology, North-Western Division of the Russian Academy of Medical Sciences, St. Petersburg, Russia aut Okorokova L. D. O. Ott Research Institute of Obstetrics and Gynecology, North-Western Division of the Russian Academy of Medical Sciences, St. Petersburg, Russia aut Semenov V. D. O. Ott Research Institute of Obstetrics and Gynecology, North-Western Division of the Russian Academy of Medical Sciences, St. Petersburg, Russia aut Rebezova E. D. O. Ott Research Institute of Obstetrics and Gynecology, North-Western Division of the Russian Academy of Medical Sciences, St. Petersburg, Russia aut Sel'kov S. D. O. Ott Research Institute of Obstetrics and Gynecology, North-Western Division of the Russian Academy of Medical Sciences, St. Petersburg, Russia aut Sokolov D. D. O. Ott Research Institute of Obstetrics and Gynecology, North-Western Division of the Russian Academy of Medical Sciences, St. Petersburg, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/1(2015-05-01), 146-151 0007-4888 159:1<146 2015 159 10517 https://doi.org/10.1007/s10517-015-2911-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-2911-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- L'vova T. D. O. Ott Research Institute of Obstetrics and Gynecology, North-Western Division of the Russian Academy of Medical Sciences, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Stepanova O. D. O. Ott Research Institute of Obstetrics and Gynecology, North-Western Division of the Russian Academy of Medical Sciences, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Okorokova L. D. O. Ott Research Institute of Obstetrics and Gynecology, North-Western Division of the Russian Academy of Medical Sciences, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Semenov V. D. O. Ott Research Institute of Obstetrics and Gynecology, North-Western Division of the Russian Academy of Medical Sciences, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Rebezova E. D. O. Ott Research Institute of Obstetrics and Gynecology, North-Western Division of the Russian Academy of Medical Sciences, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Sel'kov S. D. O. Ott Research Institute of Obstetrics and Gynecology, North-Western Division of the Russian Academy of Medical Sciences, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Sokolov D. D. O. Ott Research Institute of Obstetrics and Gynecology, North-Western Division of the Russian Academy of Medical Sciences, St. Petersburg, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/1(2015-05-01), 146-151 0007-4888 159:1<146 2015 159 10517