Optimization of the Method of Buccal Epithelial Cell Isolation and Culturing on Collagen Substrate for Ophthalmologic Application
Gespeichert in:
Verfasser / Beitragende:
[E. Chentsova, O. Konyushko, M. Makarov, N. Egorova, M. Zinov'ev, N. Borovkova]
Ort, Verlag, Jahr:
2015
Enthalten in:
Bulletin of Experimental Biology and Medicine, 159/1(2015-05-01), 168-172
Format:
Artikel (online)
Online Zugang:
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| 024 | 7 | 0 | |a 10.1007/s10517-015-2915-8 |2 doi |
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| 245 | 0 | 0 | |a Optimization of the Method of Buccal Epithelial Cell Isolation and Culturing on Collagen Substrate for Ophthalmologic Application |h [Elektronische Daten] |c [E. Chentsova, O. Konyushko, M. Makarov, N. Egorova, M. Zinov'ev, N. Borovkova] |
| 520 | 3 | |a We performed morphofunctional analysis of cultured rabbit buccal epithelial cells in the primary culture, during passaging, and upon interaction with collagen substrates. Three different morphological types were identified. It was demonstrated that precursors of rabbit buccal epithelium were characterized by high proliferative activity, retain their biological properties throughout long-term culturing, and adhere to various collagen substrates. | |
| 540 | |a Springer Science+Business Media New York, 2015 | ||
| 690 | 7 | |a buccal epithelium |2 nationallicence | |
| 690 | 7 | |a cells |2 nationallicence | |
| 690 | 7 | |a nucleus-cytoplasm ratio |2 nationallicence | |
| 690 | 7 | |a cell membrane integrity |2 nationallicence | |
| 690 | 7 | |a collagen substrate |2 nationallicence | |
| 700 | 1 | |a Chentsova |D E. |u Helmholtz Moscow Research Institute of Eye Diseases, Ministry of Health of the Russian Federation, Moscow, Russia |4 aut | |
| 700 | 1 | |a Konyushko |D O. |u Helmholtz Moscow Research Institute of Eye Diseases, Ministry of Health of the Russian Federation, Moscow, Russia |4 aut | |
| 700 | 1 | |a Makarov |D M. |u N. V. Sklifosovskii Research Institute of Emergency Medical Care, Moscow, Russia |4 aut | |
| 700 | 1 | |a Egorova |D N. |u Helmholtz Moscow Research Institute of Eye Diseases, Ministry of Health of the Russian Federation, Moscow, Russia |4 aut | |
| 700 | 1 | |a Zinov'ev |D M. |u Helmholtz Moscow Research Institute of Eye Diseases, Ministry of Health of the Russian Federation, Moscow, Russia |4 aut | |
| 700 | 1 | |a Borovkova |D N. |u Helmholtz Moscow Research Institute of Eye Diseases, Ministry of Health of the Russian Federation, Moscow, Russia |4 aut | |
| 773 | 0 | |t Bulletin of Experimental Biology and Medicine |d Springer US; http://www.springer-ny.com |g 159/1(2015-05-01), 168-172 |x 0007-4888 |q 159:1<168 |1 2015 |2 159 |o 10517 | |
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| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Chentsova |D E. |u Helmholtz Moscow Research Institute of Eye Diseases, Ministry of Health of the Russian Federation, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Konyushko |D O. |u Helmholtz Moscow Research Institute of Eye Diseases, Ministry of Health of the Russian Federation, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Makarov |D M. |u N. V. Sklifosovskii Research Institute of Emergency Medical Care, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Egorova |D N. |u Helmholtz Moscow Research Institute of Eye Diseases, Ministry of Health of the Russian Federation, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zinov'ev |D M. |u Helmholtz Moscow Research Institute of Eye Diseases, Ministry of Health of the Russian Federation, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Borovkova |D N. |u Helmholtz Moscow Research Institute of Eye Diseases, Ministry of Health of the Russian Federation, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Bulletin of Experimental Biology and Medicine |d Springer US; http://www.springer-ny.com |g 159/1(2015-05-01), 168-172 |x 0007-4888 |q 159:1<168 |1 2015 |2 159 |o 10517 | ||