Optimization of the Method of Buccal Epithelial Cell Isolation and Culturing on Collagen Substrate for Ophthalmologic Application

Verfasser / Beitragende:
[E. Chentsova, O. Konyushko, M. Makarov, N. Egorova, M. Zinov'ev, N. Borovkova]
Ort, Verlag, Jahr:
2015
Enthalten in:
Bulletin of Experimental Biology and Medicine, 159/1(2015-05-01), 168-172
Format:
Artikel (online)
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024 7 0 |a 10.1007/s10517-015-2915-8  |2 doi 
035 |a (NATIONALLICENCE)springer-10.1007/s10517-015-2915-8 
245 0 0 |a Optimization of the Method of Buccal Epithelial Cell Isolation and Culturing on Collagen Substrate for Ophthalmologic Application  |h [Elektronische Daten]  |c [E. Chentsova, O. Konyushko, M. Makarov, N. Egorova, M. Zinov'ev, N. Borovkova] 
520 3 |a We performed morphofunctional analysis of cultured rabbit buccal epithelial cells in the primary culture, during passaging, and upon interaction with collagen substrates. Three different morphological types were identified. It was demonstrated that precursors of rabbit buccal epithelium were characterized by high proliferative activity, retain their biological properties throughout long-term culturing, and adhere to various collagen substrates. 
540 |a Springer Science+Business Media New York, 2015 
690 7 |a buccal epithelium  |2 nationallicence 
690 7 |a cells  |2 nationallicence 
690 7 |a nucleus-cytoplasm ratio  |2 nationallicence 
690 7 |a cell membrane integrity  |2 nationallicence 
690 7 |a collagen substrate  |2 nationallicence 
700 1 |a Chentsova  |D E.  |u Helmholtz Moscow Research Institute of Eye Diseases, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
700 1 |a Konyushko  |D O.  |u Helmholtz Moscow Research Institute of Eye Diseases, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
700 1 |a Makarov  |D M.  |u N. V. Sklifosovskii Research Institute of Emergency Medical Care, Moscow, Russia  |4 aut 
700 1 |a Egorova  |D N.  |u Helmholtz Moscow Research Institute of Eye Diseases, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
700 1 |a Zinov'ev  |D M.  |u Helmholtz Moscow Research Institute of Eye Diseases, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
700 1 |a Borovkova  |D N.  |u Helmholtz Moscow Research Institute of Eye Diseases, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
773 0 |t Bulletin of Experimental Biology and Medicine  |d Springer US; http://www.springer-ny.com  |g 159/1(2015-05-01), 168-172  |x 0007-4888  |q 159:1<168  |1 2015  |2 159  |o 10517 
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908 |D 1  |a research-article  |2 jats 
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950 |B NATIONALLICENCE  |P 700  |E 1-  |a Chentsova  |D E.  |u Helmholtz Moscow Research Institute of Eye Diseases, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Konyushko  |D O.  |u Helmholtz Moscow Research Institute of Eye Diseases, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Makarov  |D M.  |u N. V. Sklifosovskii Research Institute of Emergency Medical Care, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Egorova  |D N.  |u Helmholtz Moscow Research Institute of Eye Diseases, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Zinov'ev  |D M.  |u Helmholtz Moscow Research Institute of Eye Diseases, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Borovkova  |D N.  |u Helmholtz Moscow Research Institute of Eye Diseases, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 773  |E 0-  |t Bulletin of Experimental Biology and Medicine  |d Springer US; http://www.springer-ny.com  |g 159/1(2015-05-01), 168-172  |x 0007-4888  |q 159:1<168  |1 2015  |2 159  |o 10517