caa a22 4500 60553473X CHVBK 20210128100844.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s10517-015-2916-7 doi (NATIONALLICENCE)springer-10.1007/s10517-015-2916-7 Functionally Active Gap Junctions between Connexin 43-Positive Mesenchymal Stem Cells and Glioma Cells [Elektronische Daten] [A. Gabashvili, V. Baklaushev, N. Grinenko, A. Levinskii, P. Mel'nikov, S. Cherepanov, V. Chekhonin] The formation of functional gap junctions between mesenchymal stem cells and cells of low-grade rat glioma C6 cells was studied in in vitro experiments. Immunocytochemical analysis with antibodies to connexin 43 extracellular loop 2 showed that mesenchymal stem cells as well as C6 glioma cells express the main astroglial gap junction protein connexin 43. Analysis of migration activity showed that mesenchymal stem cells actively migrate towards C6 glioma cells. During co-culturing, mesenchymal stem cells and glioma C6 form functionally active gap junctions mediating the transport of cytoplasmic dye from glioma cells to mesenchymal stem cells in the opposite direction. Fluorometry showed that the intensity of transport of low-molecular substances through heterologous gap junctions between mesenchymal stem cells and glioma cells is similar to that through homologous gap junctions between glioma cells. This phenomenon can be used for the development of new methods of cell therapy of high-grade gliomas. Springer Science+Business Media New York, 2015 mesenchymal stem cells nationallicence glioblastoma multiforme nationallicence C6 glioma nationallicence connexin 43 nationallicence gap junctions nationallicence Gabashvili A. Department of Medical Nanobiotechnologies, Medico-Biological Faculty, N. I. Pirogov Russian National Research Medical University, Moscow, Russia aut Baklaushev V. Department of Medical Nanobiotechnologies, Medico-Biological Faculty, N. I. Pirogov Russian National Research Medical University, Moscow, Russia aut Grinenko N. Department of Fundamental and Applied Neurobiology, V. P. Serbsky State Research Center of Forensic and Social Psychiatry, Moscow, Russia aut Levinskii A. Department of Medical Nanobiotechnologies, Medico-Biological Faculty, N. I. Pirogov Russian National Research Medical University, Moscow, Russia aut Mel'nikov P. Department of Medical Nanobiotechnologies, Medico-Biological Faculty, N. I. Pirogov Russian National Research Medical University, Moscow, Russia aut Cherepanov S. Department of Medical Nanobiotechnologies, Medico-Biological Faculty, N. I. Pirogov Russian National Research Medical University, Moscow, Russia aut Chekhonin V. Department of Medical Nanobiotechnologies, Medico-Biological Faculty, N. I. Pirogov Russian National Research Medical University, Moscow, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/1(2015-05-01), 173-179 0007-4888 159:1<173 2015 159 10517 https://doi.org/10.1007/s10517-015-2916-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-2916-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gabashvili A. Department of Medical Nanobiotechnologies, Medico-Biological Faculty, N. I. Pirogov Russian National Research Medical University, Moscow, Russia aut NATIONALLICENCE 700 1- Baklaushev V. Department of Medical Nanobiotechnologies, Medico-Biological Faculty, N. I. Pirogov Russian National Research Medical University, Moscow, Russia aut NATIONALLICENCE 700 1- Grinenko N. Department of Fundamental and Applied Neurobiology, V. P. Serbsky State Research Center of Forensic and Social Psychiatry, Moscow, Russia aut NATIONALLICENCE 700 1- Levinskii A. Department of Medical Nanobiotechnologies, Medico-Biological Faculty, N. I. Pirogov Russian National Research Medical University, Moscow, Russia aut NATIONALLICENCE 700 1- Mel'nikov P. Department of Medical Nanobiotechnologies, Medico-Biological Faculty, N. I. Pirogov Russian National Research Medical University, Moscow, Russia aut NATIONALLICENCE 700 1- Cherepanov S. Department of Medical Nanobiotechnologies, Medico-Biological Faculty, N. I. Pirogov Russian National Research Medical University, Moscow, Russia aut NATIONALLICENCE 700 1- Chekhonin V. Department of Medical Nanobiotechnologies, Medico-Biological Faculty, N. I. Pirogov Russian National Research Medical University, Moscow, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/1(2015-05-01), 173-179 0007-4888 159:1<173 2015 159 10517